Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5643 and PP_5644 are separated by 2 nucleotides PP_5644 and PP_3899 are separated by 167 nucleotides PP_3899 and PP_3900 are separated by 8 nucleotides PP_3900 and PP_3901 are separated by 149 nucleotides PP_3901 and PP_3902 are separated by 118 nucleotides
PP_5643: PP_5643 - conserved protein of unknown function, at 4,413,330 to 4,414,322
_5643
PP_5644: PP_5644 - conserved membrane protein of unknown function, at 4,414,325 to 4,414,891
_5644
PP_3899: PP_3899 - conserved protein of unknown function, hicB family, at 4,415,059 to 4,415,391
_3899
PP_3900: PP_3900 - hypothetical protein, at 4,415,400 to 4,415,648
_3900
PP_3901: PP_3901 - conserved protein of unknown function, at 4,415,798 to 4,416,058
_3901
PP_3902: PP_3902 - conserved protein of unknown function, at 4,416,177 to 4,416,485
_3902
Position (kb)
4415
4416 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4414.060 kb on + strand, within PP_5643 at 4414.061 kb on - strand, within PP_5643 at 4414.063 kb on + strand, within PP_5643 at 4414.064 kb on - strand, within PP_5643 at 4414.064 kb on - strand at 4414.125 kb on + strand, within PP_5643 at 4414.126 kb on - strand, within PP_5643 at 4414.143 kb on - strand, within PP_5643 at 4414.148 kb on + strand, within PP_5643 at 4414.148 kb on + strand, within PP_5643 at 4414.182 kb on - strand, within PP_5643 at 4414.190 kb on + strand, within PP_5643 at 4414.217 kb on + strand, within PP_5643 at 4414.218 kb on - strand, within PP_5643 at 4414.218 kb on - strand, within PP_5643 at 4414.218 kb on - strand, within PP_5643 at 4414.218 kb on - strand, within PP_5643 at 4414.218 kb on - strand, within PP_5643 at 4414.241 kb on - strand at 4414.241 kb on - strand at 4414.241 kb on - strand at 4414.241 kb on - strand at 4414.342 kb on - strand at 4414.432 kb on - strand, within PP_5644 at 4414.437 kb on + strand, within PP_5644 at 4414.489 kb on - strand, within PP_5644 at 4414.814 kb on + strand, within PP_5644 at 4414.863 kb on - strand at 4414.863 kb on - strand at 4414.866 kb on - strand at 4414.869 kb on + strand at 4414.870 kb on - strand at 4414.874 kb on + strand at 4415.018 kb on + strand at 4415.018 kb on + strand at 4415.019 kb on - strand at 4415.078 kb on + strand at 4415.079 kb on - strand at 4415.079 kb on - strand at 4415.150 kb on - strand, within PP_3899 at 4415.150 kb on - strand, within PP_3899 at 4415.430 kb on + strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.431 kb on - strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.433 kb on + strand, within PP_3900 at 4415.434 kb on - strand, within PP_3900 at 4415.445 kb on - strand, within PP_3900 at 4415.450 kb on - strand, within PP_3900 at 4415.642 kb on + strand at 4415.643 kb on - strand at 4415.643 kb on - strand at 4415.736 kb on + strand at 4415.736 kb on + strand at 4415.737 kb on - strand at 4415.746 kb on + strand at 4415.746 kb on + strand at 4415.747 kb on - strand at 4415.775 kb on - strand at 4415.792 kb on - strand at 4415.792 kb on - strand at 4415.803 kb on + strand at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.924 kb on + strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.925 kb on - strand, within PP_3901 at 4415.994 kb on + strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4415.995 kb on - strand, within PP_3901 at 4416.049 kb on - strand at 4416.107 kb on + strand at 4416.126 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 4,414,060 + PP_5643 0.74 +0.5 4,414,061 - PP_5643 0.74 +0.8 4,414,063 + PP_5643 0.74 -1.3 4,414,064 - PP_5643 0.74 +3.1 4,414,064 - +2.3 4,414,125 + PP_5643 0.80 -0.1 4,414,126 - PP_5643 0.80 -2.3 4,414,143 - PP_5643 0.82 -1.9 4,414,148 + PP_5643 0.82 -0.4 4,414,148 + PP_5643 0.82 -0.6 4,414,182 - PP_5643 0.86 +1.0 4,414,190 + PP_5643 0.87 +0.4 4,414,217 + PP_5643 0.89 +1.1 4,414,218 - PP_5643 0.89 +0.3 4,414,218 - PP_5643 0.89 -0.6 4,414,218 - PP_5643 0.89 -2.2 4,414,218 - PP_5643 0.89 -0.3 4,414,218 - PP_5643 0.89 -0.5 4,414,241 - +0.0 4,414,241 - -1.0 4,414,241 - +0.1 4,414,241 - +0.6 4,414,342 - +0.4 4,414,432 - PP_5644 0.19 +0.9 4,414,437 + PP_5644 0.20 -0.0 4,414,489 - PP_5644 0.29 -0.4 4,414,814 + PP_5644 0.86 +1.1 4,414,863 - -1.1 4,414,863 - -1.2 4,414,866 - -1.3 4,414,869 + -0.1 4,414,870 - +1.8 4,414,874 + -1.5 4,415,018 + -0.5 4,415,018 + +0.7 4,415,019 - +1.3 4,415,078 + +0.1 4,415,079 - +0.5 4,415,079 - -0.6 4,415,150 - PP_3899 0.27 +2.5 4,415,150 - PP_3899 0.27 +0.3 4,415,430 + PP_3900 0.12 +0.8 4,415,431 - PP_3900 0.12 -0.4 4,415,431 - PP_3900 0.12 -0.1 4,415,431 - PP_3900 0.12 +0.0 4,415,433 + PP_3900 0.13 -0.1 4,415,433 + PP_3900 0.13 -0.6 4,415,434 - PP_3900 0.14 -0.7 4,415,445 - PP_3900 0.18 -1.0 4,415,450 - PP_3900 0.20 +1.3 4,415,642 + -2.1 4,415,643 - +0.1 4,415,643 - +0.0 4,415,736 + +0.8 4,415,736 + +1.5 4,415,737 - -0.5 4,415,746 + +0.5 4,415,746 + +1.0 4,415,747 - +0.2 4,415,775 - -1.6 4,415,792 - -0.2 4,415,792 - -1.0 4,415,803 + +0.3 4,415,924 + PP_3901 0.48 -0.5 4,415,924 + PP_3901 0.48 -0.0 4,415,924 + PP_3901 0.48 -0.9 4,415,925 - PP_3901 0.49 +0.0 4,415,925 - PP_3901 0.49 +1.0 4,415,994 + PP_3901 0.75 -2.0 4,415,995 - PP_3901 0.75 +2.0 4,415,995 - PP_3901 0.75 +0.1 4,415,995 - PP_3901 0.75 +0.8 4,415,995 - PP_3901 0.75 -0.0 4,416,049 - +0.7 4,416,107 + +0.9 4,416,126 + -0.7
Or see this region's nucleotide sequence