Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3886 and PP_3887 overlap by 17 nucleotides PP_3887 and PP_3888 are separated by 12 nucleotides PP_3888 and PP_3889 overlap by 4 nucleotides
PP_3886: PP_3886 - conserved protein of unknown function, at 4,405,300 to 4,405,560
_3886
PP_3887: PP_3887 - conserved protein of unknown function, at 4,405,544 to 4,406,215
_3887
PP_3888: PP_3888 - conserved protein of unknown function, at 4,406,228 to 4,406,548
_3888
PP_3889: PP_3889 - putative Phage integrase, at 4,406,545 to 4,407,843
_3889
Position (kb)
4406
4407 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4405.267 kb on + strand at 4405.267 kb on + strand at 4405.268 kb on - strand at 4405.268 kb on - strand at 4405.268 kb on - strand at 4405.268 kb on - strand at 4405.281 kb on + strand at 4405.282 kb on - strand at 4405.404 kb on + strand, within PP_3886 at 4405.625 kb on - strand, within PP_3887 at 4405.762 kb on + strand, within PP_3887 at 4405.794 kb on + strand, within PP_3887 at 4405.794 kb on + strand, within PP_3887 at 4405.896 kb on + strand, within PP_3887 at 4405.896 kb on + strand, within PP_3887 at 4405.897 kb on - strand, within PP_3887 at 4405.907 kb on - strand, within PP_3887 at 4405.951 kb on + strand, within PP_3887 at 4405.952 kb on - strand, within PP_3887 at 4405.955 kb on + strand, within PP_3887 at 4405.955 kb on + strand, within PP_3887 at 4405.993 kb on + strand, within PP_3887 at 4405.993 kb on + strand, within PP_3887 at 4405.993 kb on + strand, within PP_3887 at 4405.994 kb on - strand, within PP_3887 at 4405.994 kb on - strand, within PP_3887 at 4406.043 kb on + strand, within PP_3887 at 4406.044 kb on - strand, within PP_3887 at 4406.044 kb on - strand, within PP_3887 at 4406.207 kb on + strand at 4406.280 kb on + strand, within PP_3888 at 4406.280 kb on + strand, within PP_3888 at 4406.282 kb on + strand, within PP_3888 at 4406.282 kb on + strand, within PP_3888 at 4406.282 kb on + strand, within PP_3888 at 4406.386 kb on + strand, within PP_3888 at 4406.386 kb on + strand, within PP_3888 at 4406.635 kb on + strand at 4406.635 kb on + strand at 4406.741 kb on + strand, within PP_3889 at 4406.741 kb on + strand, within PP_3889 at 4406.741 kb on + strand, within PP_3889 at 4406.741 kb on + strand, within PP_3889 at 4406.936 kb on + strand, within PP_3889 at 4406.937 kb on - strand, within PP_3889 at 4406.948 kb on + strand, within PP_3889 at 4406.948 kb on + strand, within PP_3889 at 4407.359 kb on + strand, within PP_3889 at 4407.402 kb on + strand, within PP_3889 at 4407.402 kb on + strand, within PP_3889 at 4407.402 kb on + strand, within PP_3889 at 4407.425 kb on + strand, within PP_3889 at 4407.425 kb on + strand, within PP_3889 at 4407.425 kb on + strand, within PP_3889 at 4407.426 kb on - strand, within PP_3889 at 4407.426 kb on - strand, within PP_3889 at 4407.445 kb on + strand, within PP_3889 at 4407.446 kb on - strand, within PP_3889 at 4407.492 kb on + strand, within PP_3889 at 4407.492 kb on + strand, within PP_3889 at 4407.492 kb on + strand, within PP_3889 at 4407.493 kb on - strand, within PP_3889 at 4407.493 kb on - strand, within PP_3889 at 4407.493 kb on - strand, within PP_3889 at 4407.536 kb on + strand, within PP_3889 at 4407.536 kb on + strand, within PP_3889 at 4407.536 kb on + strand at 4407.537 kb on - strand, within PP_3889
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 4,405,267 + +1.0 4,405,267 + +0.0 4,405,268 - -0.3 4,405,268 - +0.7 4,405,268 - -0.1 4,405,268 - -0.6 4,405,281 + +0.8 4,405,282 - +2.3 4,405,404 + PP_3886 0.40 -0.6 4,405,625 - PP_3887 0.12 -0.4 4,405,762 + PP_3887 0.32 -0.1 4,405,794 + PP_3887 0.37 -0.7 4,405,794 + PP_3887 0.37 -0.4 4,405,896 + PP_3887 0.52 +0.2 4,405,896 + PP_3887 0.52 -1.9 4,405,897 - PP_3887 0.53 +1.3 4,405,907 - PP_3887 0.54 +0.5 4,405,951 + PP_3887 0.61 +0.1 4,405,952 - PP_3887 0.61 -1.5 4,405,955 + PP_3887 0.61 +1.1 4,405,955 + PP_3887 0.61 +0.3 4,405,993 + PP_3887 0.67 -1.1 4,405,993 + PP_3887 0.67 -0.7 4,405,993 + PP_3887 0.67 -2.2 4,405,994 - PP_3887 0.67 -0.7 4,405,994 - PP_3887 0.67 -0.4 4,406,043 + PP_3887 0.74 +0.3 4,406,044 - PP_3887 0.74 -0.2 4,406,044 - PP_3887 0.74 +0.8 4,406,207 + +0.8 4,406,280 + PP_3888 0.16 +0.2 4,406,280 + PP_3888 0.16 -1.3 4,406,282 + PP_3888 0.17 -1.7 4,406,282 + PP_3888 0.17 -1.2 4,406,282 + PP_3888 0.17 -1.7 4,406,386 + PP_3888 0.49 -1.2 4,406,386 + PP_3888 0.49 -0.7 4,406,635 + +0.6 4,406,635 + -2.0 4,406,741 + PP_3889 0.15 +0.5 4,406,741 + PP_3889 0.15 -0.1 4,406,741 + PP_3889 0.15 +0.4 4,406,741 + PP_3889 0.15 +0.2 4,406,936 + PP_3889 0.30 +1.7 4,406,937 - PP_3889 0.30 -1.9 4,406,948 + PP_3889 0.31 +0.3 4,406,948 + PP_3889 0.31 +1.3 4,407,359 + PP_3889 0.63 +4.0 4,407,402 + PP_3889 0.66 -0.5 4,407,402 + PP_3889 0.66 -1.1 4,407,402 + PP_3889 0.66 -0.8 4,407,425 + PP_3889 0.68 -0.1 4,407,425 + PP_3889 0.68 -0.9 4,407,425 + PP_3889 0.68 +0.8 4,407,426 - PP_3889 0.68 -0.3 4,407,426 - PP_3889 0.68 -0.7 4,407,445 + PP_3889 0.69 -1.9 4,407,446 - PP_3889 0.69 -0.8 4,407,492 + PP_3889 0.73 -0.8 4,407,492 + PP_3889 0.73 -0.1 4,407,492 + PP_3889 0.73 +2.6 4,407,493 - PP_3889 0.73 -0.7 4,407,493 - PP_3889 0.73 +0.4 4,407,493 - PP_3889 0.73 +0.7 4,407,536 + PP_3889 0.76 +0.3 4,407,536 + PP_3889 0.76 -0.4 4,407,536 + -0.2 4,407,537 - PP_3889 0.76 +0.2
Or see this region's nucleotide sequence