Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2645 and PP_2646 are separated by 3 nucleotides PP_2646 and PP_2647 are separated by 106 nucleotides PP_2647 and PP_2648 are separated by 292 nucleotides
PP_2645: PP_2645 - magnesium transporter, ATP-dependent, at 3,030,694 to 3,033,456
_2645
PP_2646: PP_2646 - conserved protein of unknown function, at 3,033,460 to 3,033,855
_2646
PP_2647: PP_2647 - Major facilitator family transporter, at 3,033,962 to 3,035,188
_2647
PP_2648: PP_2648 - Universal stress protein family, at 3,035,481 to 3,036,410
_2648
Position (kb)
3033
3034
3035
3036 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3032.965 kb on + strand, within PP_2645 at 3032.965 kb on + strand, within PP_2645 at 3032.965 kb on + strand, within PP_2645 at 3032.966 kb on - strand, within PP_2645 at 3033.071 kb on + strand, within PP_2645 at 3033.079 kb on - strand, within PP_2645 at 3033.122 kb on - strand, within PP_2645 at 3033.144 kb on - strand, within PP_2645 at 3033.144 kb on - strand, within PP_2645 at 3033.207 kb on - strand at 3033.207 kb on - strand at 3033.270 kb on - strand at 3033.300 kb on + strand at 3033.304 kb on + strand at 3033.304 kb on + strand at 3033.304 kb on + strand at 3033.305 kb on - strand at 3033.361 kb on + strand at 3033.362 kb on - strand at 3033.394 kb on + strand at 3033.395 kb on - strand at 3033.395 kb on - strand at 3033.427 kb on + strand at 3033.427 kb on + strand at 3033.427 kb on + strand at 3033.428 kb on - strand at 3033.428 kb on - strand at 3033.428 kb on - strand at 3033.429 kb on + strand at 3033.491 kb on + strand at 3033.491 kb on + strand at 3033.492 kb on - strand at 3033.508 kb on + strand, within PP_2646 at 3033.509 kb on - strand, within PP_2646 at 3033.811 kb on + strand, within PP_2646 at 3033.812 kb on - strand, within PP_2646 at 3033.854 kb on - strand at 3033.854 kb on - strand at 3033.874 kb on + strand at 3033.879 kb on + strand at 3033.880 kb on - strand at 3033.880 kb on - strand at 3033.886 kb on - strand at 3034.075 kb on + strand at 3034.075 kb on + strand at 3034.075 kb on + strand at 3034.076 kb on - strand at 3034.076 kb on - strand at 3034.232 kb on + strand at 3034.232 kb on + strand, within PP_2647 at 3034.392 kb on + strand, within PP_2647 at 3034.392 kb on + strand, within PP_2647 at 3034.418 kb on - strand, within PP_2647 at 3034.461 kb on + strand, within PP_2647 at 3034.461 kb on + strand, within PP_2647 at 3034.461 kb on + strand, within PP_2647 at 3034.461 kb on + strand, within PP_2647 at 3034.462 kb on - strand, within PP_2647 at 3034.467 kb on + strand, within PP_2647 at 3034.468 kb on - strand, within PP_2647 at 3034.619 kb on + strand, within PP_2647 at 3034.625 kb on + strand, within PP_2647 at 3034.625 kb on + strand, within PP_2647 at 3034.626 kb on - strand, within PP_2647 at 3034.813 kb on + strand, within PP_2647 at 3034.814 kb on - strand, within PP_2647 at 3034.920 kb on + strand, within PP_2647 at 3034.921 kb on - strand, within PP_2647 at 3035.052 kb on - strand, within PP_2647 at 3035.219 kb on - strand at 3035.255 kb on + strand at 3035.297 kb on + strand at 3035.297 kb on + strand at 3035.330 kb on + strand at 3035.482 kb on + strand at 3035.490 kb on + strand at 3035.490 kb on + strand at 3035.521 kb on - strand at 3035.704 kb on - strand, within PP_2648 at 3036.108 kb on - strand, within PP_2648 at 3036.112 kb on + strand, within PP_2648
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,032,965 + PP_2645 0.82 -0.8 3,032,965 + PP_2645 0.82 -1.6 3,032,965 + PP_2645 0.82 +0.7 3,032,966 - PP_2645 0.82 -1.1 3,033,071 + PP_2645 0.86 +0.5 3,033,079 - PP_2645 0.86 -1.0 3,033,122 - PP_2645 0.88 +0.5 3,033,144 - PP_2645 0.89 -1.3 3,033,144 - PP_2645 0.89 +1.0 3,033,207 - +1.7 3,033,207 - -0.8 3,033,270 - +1.0 3,033,300 + +0.6 3,033,304 + -0.0 3,033,304 + +0.2 3,033,304 + +0.1 3,033,305 - -0.6 3,033,361 + +0.4 3,033,362 - +0.6 3,033,394 + +0.1 3,033,395 - +0.3 3,033,395 - -0.2 3,033,427 + +1.6 3,033,427 + +0.6 3,033,427 + +0.5 3,033,428 - +0.3 3,033,428 - +0.2 3,033,428 - +3.2 3,033,429 + +0.2 3,033,491 + -0.6 3,033,491 + -0.1 3,033,492 - -0.4 3,033,508 + PP_2646 0.12 -0.2 3,033,509 - PP_2646 0.12 +0.0 3,033,811 + PP_2646 0.89 +0.8 3,033,812 - PP_2646 0.89 -1.0 3,033,854 - +0.2 3,033,854 - -0.1 3,033,874 + -0.2 3,033,879 + -0.1 3,033,880 - +1.4 3,033,880 - -0.3 3,033,886 - -0.6 3,034,075 + +1.8 3,034,075 + +3.2 3,034,075 + +0.8 3,034,076 - -0.9 3,034,076 - +0.4 3,034,232 + -0.1 3,034,232 + PP_2647 0.22 -0.6 3,034,392 + PP_2647 0.35 -1.1 3,034,392 + PP_2647 0.35 +0.7 3,034,418 - PP_2647 0.37 -0.2 3,034,461 + PP_2647 0.41 -0.4 3,034,461 + PP_2647 0.41 -1.0 3,034,461 + PP_2647 0.41 -0.1 3,034,461 + PP_2647 0.41 +0.0 3,034,462 - PP_2647 0.41 -1.6 3,034,467 + PP_2647 0.41 -0.3 3,034,468 - PP_2647 0.41 -1.0 3,034,619 + PP_2647 0.54 -1.3 3,034,625 + PP_2647 0.54 -1.5 3,034,625 + PP_2647 0.54 -0.3 3,034,626 - PP_2647 0.54 -0.1 3,034,813 + PP_2647 0.69 -1.2 3,034,814 - PP_2647 0.69 -1.6 3,034,920 + PP_2647 0.78 -0.7 3,034,921 - PP_2647 0.78 -1.1 3,035,052 - PP_2647 0.89 -0.9 3,035,219 - -0.0 3,035,255 + +0.2 3,035,297 + +0.7 3,035,297 + -0.4 3,035,330 + +0.3 3,035,482 + -0.8 3,035,490 + +0.7 3,035,490 + -0.4 3,035,521 - -0.5 3,035,704 - PP_2648 0.24 -0.8 3,036,108 - PP_2648 0.67 -0.4 3,036,112 + PP_2648 0.68 +3.0
Or see this region's nucleotide sequence