Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2618 and PP_2619 overlap by 4 nucleotides PP_2619 and PP_2620 overlap by 4 nucleotides PP_2620 and PP_2621 overlap by 37 nucleotides
PP_2618: PP_2618 - conserved exported protein of unknown function, at 2,994,467 to 2,995,264
_2618
PP_2619: PP_2619 - FHA domain protein, at 2,995,261 to 2,995,830
_2619
PP_2620: PP_2620 - conserved protein of unknown function, at 2,995,827 to 2,996,843
_2620
PP_2621: PP_2621 - conserved protein of unknown function, at 2,996,807 to 2,998,573
_2621
Position (kb)
2995
2996
2997 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2994.858 kb on + strand, within PP_2618 at 2994.956 kb on + strand, within PP_2618 at 2994.961 kb on - strand, within PP_2618 at 2994.996 kb on + strand, within PP_2618 at 2995.269 kb on - strand at 2995.520 kb on + strand, within PP_2619 at 2995.520 kb on + strand, within PP_2619 at 2995.521 kb on - strand, within PP_2619 at 2995.539 kb on - strand, within PP_2619 at 2995.543 kb on - strand at 2995.543 kb on - strand, within PP_2619 at 2995.624 kb on - strand, within PP_2619 at 2995.624 kb on - strand, within PP_2619 at 2995.776 kb on + strand at 2995.904 kb on - strand at 2995.972 kb on + strand, within PP_2620 at 2995.973 kb on - strand, within PP_2620 at 2995.973 kb on - strand, within PP_2620 at 2996.260 kb on - strand, within PP_2620 at 2996.422 kb on + strand, within PP_2620 at 2996.422 kb on + strand, within PP_2620 at 2996.422 kb on + strand, within PP_2620 at 2996.422 kb on + strand, within PP_2620 at 2996.422 kb on + strand, within PP_2620 at 2996.425 kb on + strand, within PP_2620 at 2996.425 kb on + strand, within PP_2620 at 2996.431 kb on + strand, within PP_2620 at 2996.431 kb on + strand at 2996.444 kb on + strand, within PP_2620 at 2996.468 kb on - strand, within PP_2620 at 2996.468 kb on - strand, within PP_2620 at 2996.543 kb on - strand, within PP_2620 at 2996.545 kb on + strand, within PP_2620 at 2996.546 kb on - strand, within PP_2620 at 2996.638 kb on + strand, within PP_2620 at 2996.639 kb on - strand, within PP_2620 at 2996.777 kb on - strand at 2996.808 kb on + strand at 2996.834 kb on + strand at 2996.835 kb on - strand at 2996.848 kb on - strand at 2996.848 kb on - strand at 2996.907 kb on + strand at 2996.946 kb on + strand at 2996.946 kb on + strand at 2996.947 kb on - strand at 2997.046 kb on + strand, within PP_2621 at 2997.047 kb on - strand, within PP_2621 at 2997.047 kb on - strand, within PP_2621 at 2997.061 kb on + strand, within PP_2621 at 2997.171 kb on + strand, within PP_2621 at 2997.171 kb on + strand, within PP_2621 at 2997.172 kb on - strand, within PP_2621 at 2997.172 kb on - strand, within PP_2621 at 2997.172 kb on - strand, within PP_2621 at 2997.172 kb on - strand, within PP_2621 at 2997.172 kb on - strand, within PP_2621 at 2997.247 kb on + strand, within PP_2621 at 2997.261 kb on + strand, within PP_2621 at 2997.261 kb on + strand, within PP_2621 at 2997.261 kb on + strand at 2997.281 kb on - strand, within PP_2621 at 2997.377 kb on + strand at 2997.378 kb on - strand, within PP_2621 at 2997.381 kb on + strand, within PP_2621 at 2997.382 kb on - strand, within PP_2621 at 2997.465 kb on + strand, within PP_2621 at 2997.550 kb on + strand, within PP_2621 at 2997.550 kb on + strand, within PP_2621 at 2997.782 kb on + strand, within PP_2621
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,994,858 + PP_2618 0.49 +1.7 2,994,956 + PP_2618 0.61 -0.6 2,994,961 - PP_2618 0.62 +0.6 2,994,996 + PP_2618 0.66 -0.3 2,995,269 - -1.3 2,995,520 + PP_2619 0.45 -0.4 2,995,520 + PP_2619 0.45 -0.9 2,995,521 - PP_2619 0.46 -0.1 2,995,539 - PP_2619 0.49 -1.2 2,995,543 - +1.0 2,995,543 - PP_2619 0.49 -0.1 2,995,624 - PP_2619 0.64 -1.5 2,995,624 - PP_2619 0.64 -0.5 2,995,776 + +0.8 2,995,904 - -0.6 2,995,972 + PP_2620 0.14 +3.4 2,995,973 - PP_2620 0.14 -1.0 2,995,973 - PP_2620 0.14 +0.4 2,996,260 - PP_2620 0.43 +0.1 2,996,422 + PP_2620 0.59 +1.2 2,996,422 + PP_2620 0.59 +1.0 2,996,422 + PP_2620 0.59 -1.4 2,996,422 + PP_2620 0.59 +1.5 2,996,422 + PP_2620 0.59 -0.8 2,996,425 + PP_2620 0.59 -0.0 2,996,425 + PP_2620 0.59 -1.3 2,996,431 + PP_2620 0.59 +3.3 2,996,431 + -0.1 2,996,444 + PP_2620 0.61 +0.2 2,996,468 - PP_2620 0.63 +1.6 2,996,468 - PP_2620 0.63 -0.8 2,996,543 - PP_2620 0.70 +0.1 2,996,545 + PP_2620 0.71 +0.5 2,996,546 - PP_2620 0.71 +0.5 2,996,638 + PP_2620 0.80 +1.3 2,996,639 - PP_2620 0.80 -0.1 2,996,777 - -1.5 2,996,808 + +1.2 2,996,834 + -1.9 2,996,835 - +2.6 2,996,848 - -0.8 2,996,848 - -1.4 2,996,907 + +0.4 2,996,946 + -1.1 2,996,946 + -0.0 2,996,947 - +0.7 2,997,046 + PP_2621 0.14 +2.3 2,997,047 - PP_2621 0.14 -0.1 2,997,047 - PP_2621 0.14 -0.8 2,997,061 + PP_2621 0.14 -0.8 2,997,171 + PP_2621 0.21 +0.8 2,997,171 + PP_2621 0.21 -1.4 2,997,172 - PP_2621 0.21 +1.0 2,997,172 - PP_2621 0.21 -0.8 2,997,172 - PP_2621 0.21 -1.0 2,997,172 - PP_2621 0.21 -1.6 2,997,172 - PP_2621 0.21 -1.2 2,997,247 + PP_2621 0.25 +0.2 2,997,261 + PP_2621 0.26 -0.9 2,997,261 + PP_2621 0.26 +0.4 2,997,261 + +0.0 2,997,281 - PP_2621 0.27 -1.5 2,997,377 + +1.0 2,997,378 - PP_2621 0.32 -0.6 2,997,381 + PP_2621 0.32 -0.6 2,997,382 - PP_2621 0.33 -0.8 2,997,465 + PP_2621 0.37 +0.2 2,997,550 + PP_2621 0.42 -2.9 2,997,550 + PP_2621 0.42 +0.6 2,997,782 + PP_2621 0.55 -0.3
Or see this region's nucleotide sequence