Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5533 and PP_2508 are separated by 398 nucleotides PP_2508 and PP_5534 overlap by 4 nucleotides PP_5534 and PP_2509 overlap by 4 nucleotides
PP_5533: PP_5533 - conserved protein of unknown function, at 2,855,535 to 2,855,777
_5533
PP_2508: PP_2508 - conserved protein of unknown function, at 2,856,176 to 2,856,343
_2508
PP_5534: PP_5534 - protein of unknown function, at 2,856,340 to 2,856,570
_5534
PP_2509: PP_2509 - conserved protein of unknown function, at 2,856,567 to 2,856,731
_2509
Position (kb)
2856
2857 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2855.578 kb on - strand, within PP_5533 at 2855.783 kb on - strand at 2855.807 kb on + strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.808 kb on - strand at 2855.845 kb on + strand at 2855.903 kb on - strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.173 kb on + strand at 2856.174 kb on - strand at 2856.174 kb on - strand at 2856.422 kb on + strand, within PP_5534 at 2856.422 kb on + strand, within PP_5534 at 2856.422 kb on + strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.423 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.470 kb on - strand, within PP_5534 at 2856.569 kb on - strand at 2856.576 kb on + strand at 2856.577 kb on - strand at 2856.577 kb on - strand at 2856.653 kb on + strand, within PP_2509 at 2856.654 kb on - strand, within PP_2509 at 2856.654 kb on - strand, within PP_2509 at 2856.658 kb on - strand, within PP_2509 at 2856.742 kb on - strand at 2856.742 kb on - strand at 2856.742 kb on - strand at 2856.770 kb on + strand at 2856.771 kb on - strand at 2856.771 kb on - strand at 2856.775 kb on - strand at 2856.798 kb on + strand at 2856.798 kb on + strand at 2856.798 kb on + strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.799 kb on - strand at 2856.811 kb on + strand at 2856.812 kb on - strand at 2856.812 kb on - strand at 2856.827 kb on - strand at 2856.912 kb on - strand at 2856.923 kb on + strand at 2856.923 kb on + strand at 2856.923 kb on + strand at 2856.924 kb on - strand at 2856.966 kb on + strand at 2856.967 kb on - strand at 2856.967 kb on - strand at 2857.099 kb on - strand at 2857.131 kb on + strand at 2857.167 kb on - strand at 2857.170 kb on + strand at 2857.171 kb on - strand at 2857.190 kb on - strand at 2857.229 kb on + strand at 2857.355 kb on - strand at 2857.388 kb on + strand at 2857.389 kb on - strand at 2857.389 kb on - strand at 2857.483 kb on + strand at 2857.483 kb on + strand at 2857.484 kb on - strand at 2857.524 kb on + strand at 2857.552 kb on + strand at 2857.564 kb on + strand at 2857.662 kb on + strand at 2857.686 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,855,578 - PP_5533 0.18 -0.4 2,855,783 - -0.9 2,855,807 + -0.1 2,855,808 - -0.5 2,855,808 - +1.5 2,855,808 - -0.1 2,855,845 + +0.5 2,855,903 - -0.4 2,856,173 + -1.1 2,856,173 + -0.5 2,856,173 + -1.0 2,856,174 - +0.5 2,856,174 - -0.3 2,856,422 + PP_5534 0.35 -0.1 2,856,422 + PP_5534 0.35 -0.3 2,856,422 + PP_5534 0.35 -1.3 2,856,423 - PP_5534 0.36 +1.3 2,856,423 - PP_5534 0.36 +1.2 2,856,423 - PP_5534 0.36 +0.6 2,856,423 - PP_5534 0.36 -0.9 2,856,423 - PP_5534 0.36 -0.1 2,856,423 - PP_5534 0.36 +0.4 2,856,470 - PP_5534 0.56 -1.8 2,856,470 - PP_5534 0.56 +0.2 2,856,470 - PP_5534 0.56 +0.4 2,856,569 - -0.6 2,856,576 + +0.9 2,856,577 - -1.6 2,856,577 - -1.6 2,856,653 + PP_2509 0.52 +0.7 2,856,654 - PP_2509 0.53 +1.4 2,856,654 - PP_2509 0.53 -0.3 2,856,658 - PP_2509 0.55 +0.6 2,856,742 - +1.6 2,856,742 - -1.1 2,856,742 - +0.7 2,856,770 + +0.9 2,856,771 - -0.2 2,856,771 - -0.2 2,856,775 - +0.3 2,856,798 + +1.0 2,856,798 + -0.7 2,856,798 + +1.6 2,856,799 - +1.0 2,856,799 - -1.5 2,856,799 - -0.1 2,856,799 - +0.3 2,856,799 - -0.1 2,856,799 - +2.3 2,856,799 - +0.4 2,856,811 + -0.2 2,856,812 - -0.0 2,856,812 - -0.1 2,856,827 - +0.5 2,856,912 - -0.2 2,856,923 + +0.7 2,856,923 + +2.7 2,856,923 + -0.5 2,856,924 - +0.0 2,856,966 + -1.2 2,856,967 - -1.2 2,856,967 - +0.7 2,857,099 - +0.6 2,857,131 + -0.8 2,857,167 - -0.1 2,857,170 + +1.0 2,857,171 - +0.5 2,857,190 - -0.0 2,857,229 + -2.0 2,857,355 - -0.5 2,857,388 + -0.5 2,857,389 - +0.4 2,857,389 - -0.4 2,857,483 + +2.1 2,857,483 + +0.2 2,857,484 - -1.0 2,857,524 + +0.2 2,857,552 + -2.0 2,857,564 + +2.7 2,857,662 + -0.3 2,857,686 + +0.9
Or see this region's nucleotide sequence