Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2067 and PP_2068 overlap by 11 nucleotides PP_2068 and PP_2069 are separated by 57 nucleotides
PP_2067: PP_2067 - putative EmrB/QacA family drug resistance transporter, at 2,351,750 to 2,353,264
_2067
PP_2068: PP_2068 - putative Multidrug efflux MFS membrane fusion protein, at 2,353,254 to 2,354,531
_2068
PP_2069: PP_2069 - putative Multidrug efflux MFS outer membrane protein, at 2,354,589 to 2,356,010
_2069
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2352.384 kb on + strand, within PP_2067 at 2352.384 kb on + strand, within PP_2067 at 2352.385 kb on - strand, within PP_2067 at 2352.463 kb on - strand, within PP_2067 at 2352.754 kb on - strand at 2352.774 kb on + strand, within PP_2067 at 2352.775 kb on - strand, within PP_2067 at 2352.948 kb on - strand, within PP_2067 at 2353.295 kb on + strand at 2353.295 kb on + strand at 2353.295 kb on + strand at 2353.295 kb on + strand at 2353.296 kb on - strand at 2353.320 kb on + strand at 2353.497 kb on + strand, within PP_2068 at 2353.497 kb on + strand, within PP_2068 at 2353.498 kb on - strand, within PP_2068 at 2353.498 kb on - strand, within PP_2068 at 2353.498 kb on - strand, within PP_2068 at 2353.546 kb on + strand, within PP_2068 at 2353.546 kb on + strand, within PP_2068 at 2353.547 kb on - strand, within PP_2068 at 2353.558 kb on - strand, within PP_2068 at 2353.561 kb on + strand, within PP_2068 at 2353.561 kb on + strand, within PP_2068 at 2353.562 kb on - strand, within PP_2068 at 2353.562 kb on - strand, within PP_2068 at 2353.562 kb on - strand, within PP_2068 at 2353.612 kb on + strand, within PP_2068 at 2353.612 kb on + strand, within PP_2068 at 2353.613 kb on - strand, within PP_2068 at 2353.913 kb on - strand, within PP_2068 at 2354.175 kb on + strand, within PP_2068 at 2354.175 kb on + strand, within PP_2068 at 2354.176 kb on - strand, within PP_2068 at 2354.176 kb on - strand, within PP_2068 at 2354.179 kb on + strand at 2354.180 kb on - strand, within PP_2068 at 2354.225 kb on - strand, within PP_2068 at 2354.225 kb on - strand, within PP_2068 at 2354.225 kb on - strand, within PP_2068 at 2354.343 kb on - strand, within PP_2068 at 2354.552 kb on + strand at 2354.622 kb on + strand at 2354.623 kb on - strand at 2354.731 kb on + strand at 2354.732 kb on - strand, within PP_2069 at 2354.732 kb on - strand, within PP_2069 at 2354.735 kb on + strand, within PP_2069 at 2354.736 kb on - strand, within PP_2069 at 2354.870 kb on - strand, within PP_2069 at 2355.081 kb on + strand, within PP_2069 at 2355.081 kb on + strand, within PP_2069 at 2355.081 kb on + strand, within PP_2069 at 2355.189 kb on + strand, within PP_2069 at 2355.427 kb on + strand, within PP_2069 at 2355.445 kb on + strand, within PP_2069 at 2355.446 kb on - strand, within PP_2069 at 2355.489 kb on + strand, within PP_2069 at 2355.490 kb on - strand, within PP_2069
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,352,384 + PP_2067 0.42 -1.5 2,352,384 + PP_2067 0.42 +0.9 2,352,385 - PP_2067 0.42 -1.8 2,352,463 - PP_2067 0.47 -0.8 2,352,754 - +1.3 2,352,774 + PP_2067 0.68 -2.9 2,352,775 - PP_2067 0.68 -1.7 2,352,948 - PP_2067 0.79 +0.4 2,353,295 + +1.4 2,353,295 + +1.6 2,353,295 + +0.2 2,353,295 + +0.9 2,353,296 - +1.7 2,353,320 + +0.3 2,353,497 + PP_2068 0.19 -1.0 2,353,497 + PP_2068 0.19 -0.3 2,353,498 - PP_2068 0.19 +0.3 2,353,498 - PP_2068 0.19 -2.6 2,353,498 - PP_2068 0.19 +1.6 2,353,546 + PP_2068 0.23 +0.4 2,353,546 + PP_2068 0.23 +0.7 2,353,547 - PP_2068 0.23 +0.3 2,353,558 - PP_2068 0.24 +0.2 2,353,561 + PP_2068 0.24 +1.0 2,353,561 + PP_2068 0.24 -0.3 2,353,562 - PP_2068 0.24 +0.1 2,353,562 - PP_2068 0.24 -0.0 2,353,562 - PP_2068 0.24 -0.0 2,353,612 + PP_2068 0.28 +1.8 2,353,612 + PP_2068 0.28 -1.2 2,353,613 - PP_2068 0.28 -0.5 2,353,913 - PP_2068 0.52 -1.8 2,354,175 + PP_2068 0.72 -0.8 2,354,175 + PP_2068 0.72 -0.3 2,354,176 - PP_2068 0.72 -0.7 2,354,176 - PP_2068 0.72 +0.2 2,354,179 + +1.5 2,354,180 - PP_2068 0.72 +2.7 2,354,225 - PP_2068 0.76 +0.3 2,354,225 - PP_2068 0.76 -0.9 2,354,225 - PP_2068 0.76 -0.3 2,354,343 - PP_2068 0.85 +1.2 2,354,552 + -0.4 2,354,622 + +0.5 2,354,623 - -2.0 2,354,731 + -0.8 2,354,732 - PP_2069 0.10 -0.4 2,354,732 - PP_2069 0.10 -0.6 2,354,735 + PP_2069 0.10 -0.5 2,354,736 - PP_2069 0.10 -1.5 2,354,870 - PP_2069 0.20 -0.4 2,355,081 + PP_2069 0.35 -0.4 2,355,081 + PP_2069 0.35 +0.1 2,355,081 + PP_2069 0.35 +0.9 2,355,189 + PP_2069 0.42 -0.8 2,355,427 + PP_2069 0.59 -0.0 2,355,445 + PP_2069 0.60 +0.2 2,355,446 - PP_2069 0.60 +1.1 2,355,489 + PP_2069 0.63 -0.2 2,355,490 - PP_2069 0.63 -1.4
Or see this region's nucleotide sequence