Strain Fitness in Pseudomonas putida KT2440 around PP_2010

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
2,281,142	-				-1.0
2,281,166	+				-0.8
2,281,186	+				+0.5
2,281,186	+				+0.1
2,281,186	+				+0.1
2,281,187	-				-0.5
2,281,187	-				-0.1
2,281,315	+		PP_2009	0.15	+0.6
2,281,315	+		PP_2009	0.15	+1.0
2,281,316	-		PP_2009	0.15	+0.5
2,281,516	+		PP_2009	0.38	+0.2
2,281,727	+		PP_2009	0.61	-0.7
2,281,727	+		PP_2009	0.61	+0.5
2,281,728	-		PP_2009	0.61	-0.8
2,281,745	-		PP_2009	0.63	+0.0
2,281,853	-		PP_2009	0.75	-0.6
2,281,853	-		PP_2009	0.75	-1.2
2,281,897	+		PP_2009	0.80	-0.1
2,281,897	+		PP_2009	0.80	-1.1
2,281,897	+		PP_2009	0.80	-0.0
2,281,897	+		PP_2009	0.80	+0.0
2,281,897	+		PP_2009	0.80	+1.4
2,281,898	-		PP_2009	0.80	-0.4
2,281,898	-		PP_2009	0.80	-0.2
2,281,953	+				+2.0
2,281,954	-		PP_2009	0.87	+0.1
2,281,954	-		PP_2009	0.87	-0.7
2,282,033	-				+1.4
2,282,064	+				-0.5
2,282,064	+				-0.6
2,282,065	-				-0.4
2,282,070	+				-0.4
2,282,070	+				+0.6
2,282,071	-				+1.0
2,282,109	+				+1.3
2,282,109	+				-0.6
2,282,109	+				+0.3
2,282,110	-				-1.0
2,282,118	+		PP_2010	0.10	+0.1
2,282,119	-		PP_2010	0.10	-0.5
2,282,119	-		PP_2010	0.10	+1.2
2,282,119	-		PP_2010	0.10	+1.5
2,282,119	-		PP_2010	0.10	-1.5
2,282,119	-		PP_2010	0.10	-0.0
2,282,119	-		PP_2010	0.10	+0.2
2,282,120	+		PP_2010	0.10	+0.2
2,282,120	+		PP_2010	0.10	-1.5
2,282,170	+		PP_2010	0.20	+3.9
2,282,170	+		PP_2010	0.20	+0.8
2,282,171	-		PP_2010	0.20	-1.1
2,282,316	+		PP_2010	0.46	-1.0
2,282,523	+		PP_2010	0.84	-1.4
2,282,523	+		PP_2010	0.84	+4.3
2,282,524	-		PP_2010	0.84	-0.0
2,282,580	+				+1.1
2,282,667	-				-0.6
2,282,667	-				+0.7
2,282,667	-				-0.1
2,282,853	+				-0.3
2,283,159	+		PP_2011	0.33	-0.1
2,283,160	-		PP_2011	0.33	-2.1
2,283,209	+		PP_2011	0.38	-0.6
2,283,358	-		PP_2011	0.53	+0.3
2,283,358	-		PP_2011	0.53	-0.7
2,283,516	+		PP_2011	0.69	-1.1
2,283,517	-		PP_2011	0.70	+0.4
2,283,517	-		PP_2011	0.70	+0.1
2,283,517	-		PP_2011	0.70	-0.8
2,283,517	-		PP_2011	0.70	-0.8
2,283,519	+		PP_2011	0.70	+1.8
2,283,520	-		PP_2011	0.70	-1.5
2,283,520	-		PP_2011	0.70	+0.2
2,283,520	-		PP_2011	0.70	+1.5