Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1315 and PP_1316 are separated by 14 nucleotides PP_1316 and PP_1317 are separated by 262 nucleotides PP_1317 and PP_1318 overlap by 1 nucleotides
PP_1315: PP_1315 - 50S ribosomal protein L13, at 1,503,198 to 1,503,626
_1315
PP_1316: PP_1316 - 30S ribosomal protein S9, at 1,503,641 to 1,504,033
_1316
PP_1317: PP_1317 - Ubiquinol-cytochrome c reductase iron-sulfur subunit, at 1,504,296 to 1,504,889
_1317
PP_1318: PP_1318 - Cytochrome b, at 1,504,889 to 1,506,100
_1318
Position (kb)
1504
1505 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1504.099 kb on - strand at 1504.131 kb on + strand at 1504.131 kb on + strand at 1504.137 kb on + strand at 1504.137 kb on + strand at 1504.138 kb on - strand at 1504.138 kb on - strand at 1504.175 kb on + strand at 1504.208 kb on - strand at 1504.208 kb on - strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.245 kb on + strand at 1504.246 kb on - strand at 1504.246 kb on - strand at 1504.246 kb on - strand at 1504.511 kb on - strand, within PP_1317 at 1504.608 kb on + strand, within PP_1317 at 1504.609 kb on - strand, within PP_1317 at 1504.609 kb on - strand, within PP_1317 at 1504.735 kb on + strand, within PP_1317 at 1504.735 kb on + strand, within PP_1317 at 1504.736 kb on - strand, within PP_1317 at 1504.736 kb on - strand, within PP_1317 at 1504.743 kb on + strand, within PP_1317 at 1504.770 kb on + strand, within PP_1317 at 1504.791 kb on + strand, within PP_1317 at 1504.791 kb on + strand, within PP_1317 at 1504.792 kb on - strand, within PP_1317 at 1504.839 kb on + strand at 1504.839 kb on + strand at 1504.840 kb on - strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.961 kb on + strand at 1504.962 kb on - strand at 1504.962 kb on - strand at 1504.962 kb on - strand at 1504.964 kb on + strand at 1504.964 kb on + strand at 1504.964 kb on + strand at 1504.965 kb on - strand at 1505.102 kb on + strand, within PP_1318 at 1505.103 kb on - strand, within PP_1318 at 1505.124 kb on - strand, within PP_1318 at 1505.184 kb on - strand, within PP_1318 at 1505.210 kb on + strand, within PP_1318 at 1505.211 kb on - strand, within PP_1318 at 1505.264 kb on + strand, within PP_1318 at 1505.297 kb on + strand, within PP_1318 at 1505.297 kb on + strand, within PP_1318 at 1505.324 kb on + strand, within PP_1318 at 1505.324 kb on + strand, within PP_1318 at 1505.325 kb on - strand, within PP_1318 at 1505.408 kb on + strand, within PP_1318 at 1505.408 kb on + strand, within PP_1318 at 1505.409 kb on - strand, within PP_1318 at 1505.587 kb on + strand, within PP_1318 at 1505.603 kb on + strand, within PP_1318 at 1505.603 kb on + strand, within PP_1318 at 1505.604 kb on - strand, within PP_1318 at 1505.606 kb on + strand, within PP_1318 at 1505.607 kb on - strand, within PP_1318 at 1505.607 kb on - strand, within PP_1318 at 1505.690 kb on + strand, within PP_1318 at 1505.690 kb on + strand, within PP_1318 at 1505.778 kb on - strand, within PP_1318 at 1505.778 kb on - strand, within PP_1318
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 1,504,099 - -0.3 1,504,131 + +1.0 1,504,131 + -1.3 1,504,137 + -0.3 1,504,137 + +0.5 1,504,138 - -2.2 1,504,138 - +0.3 1,504,175 + +0.4 1,504,208 - -0.7 1,504,208 - -1.7 1,504,245 + -0.6 1,504,245 + -1.5 1,504,245 + +1.8 1,504,245 + +0.5 1,504,245 + +0.8 1,504,246 - -1.1 1,504,246 - -0.4 1,504,246 - -1.0 1,504,511 - PP_1317 0.36 -2.2 1,504,608 + PP_1317 0.53 -0.5 1,504,609 - PP_1317 0.53 -1.0 1,504,609 - PP_1317 0.53 -2.1 1,504,735 + PP_1317 0.74 -1.0 1,504,735 + PP_1317 0.74 -0.4 1,504,736 - PP_1317 0.74 -1.4 1,504,736 - PP_1317 0.74 -2.6 1,504,743 + PP_1317 0.75 -0.8 1,504,770 + PP_1317 0.80 -1.4 1,504,791 + PP_1317 0.83 -0.4 1,504,791 + PP_1317 0.83 -0.6 1,504,792 - PP_1317 0.84 -2.0 1,504,839 + -0.8 1,504,839 + +0.5 1,504,840 - -0.8 1,504,961 + -1.3 1,504,961 + -0.1 1,504,961 + -1.5 1,504,961 + +0.5 1,504,962 - +1.7 1,504,962 - +0.2 1,504,962 - -0.8 1,504,964 + -1.5 1,504,964 + +1.8 1,504,964 + -1.2 1,504,965 - -0.5 1,505,102 + PP_1318 0.18 +0.3 1,505,103 - PP_1318 0.18 -0.1 1,505,124 - PP_1318 0.19 +0.6 1,505,184 - PP_1318 0.24 -1.4 1,505,210 + PP_1318 0.26 -2.3 1,505,211 - PP_1318 0.27 -0.8 1,505,264 + PP_1318 0.31 -0.6 1,505,297 + PP_1318 0.34 -2.1 1,505,297 + PP_1318 0.34 -1.0 1,505,324 + PP_1318 0.36 +3.1 1,505,324 + PP_1318 0.36 +0.2 1,505,325 - PP_1318 0.36 -0.2 1,505,408 + PP_1318 0.43 -0.9 1,505,408 + PP_1318 0.43 -2.6 1,505,409 - PP_1318 0.43 +0.2 1,505,587 + PP_1318 0.58 -2.7 1,505,603 + PP_1318 0.59 +0.0 1,505,603 + PP_1318 0.59 -1.8 1,505,604 - PP_1318 0.59 -1.9 1,505,606 + PP_1318 0.59 -1.4 1,505,607 - PP_1318 0.59 -2.3 1,505,607 - PP_1318 0.59 -1.4 1,505,690 + PP_1318 0.66 -1.4 1,505,690 + PP_1318 0.66 -0.4 1,505,778 - PP_1318 0.73 -2.6 1,505,778 - PP_1318 0.73 -2.3
Or see this region's nucleotide sequence