Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0868 and PP_0869 overlap by 4 nucleotides PP_0869 and PP_0870 overlap by 4 nucleotides PP_0870 and PP_0871 overlap by 17 nucleotides
PP_0868: PP_0868 - ABC transporter ATP-binding subunit, at 1,007,590 to 1,008,747
_0868
PP_0869: PP_0869 - osmoprotectant ABC transporter permease subunit, at 1,008,744 to 1,009,397
_0869
PP_0870: PP_0870 - putative Glycine betaine/carnitine/choline ABC transporter, periplasmic binding protein, at 1,009,394 to 1,010,335
_0870
PP_0871: PP_0871 - putative Glycine betaine/carnitine/choline ABC transporter, permease protein, at 1,010,319 to 1,011,062
_0871
Position (kb)
1008
1009
1010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1007.745 kb on - strand, within PP_0868 at 1007.745 kb on - strand, within PP_0868 at 1007.745 kb on - strand, within PP_0868 at 1007.858 kb on + strand, within PP_0868 at 1007.858 kb on + strand, within PP_0868 at 1008.170 kb on - strand, within PP_0868 at 1008.353 kb on + strand, within PP_0868 at 1008.447 kb on + strand, within PP_0868 at 1008.448 kb on - strand, within PP_0868 at 1008.448 kb on - strand, within PP_0868 at 1008.448 kb on - strand, within PP_0868 at 1008.500 kb on + strand, within PP_0868 at 1008.501 kb on - strand, within PP_0868 at 1008.522 kb on + strand, within PP_0868 at 1008.828 kb on + strand, within PP_0869 at 1008.829 kb on - strand, within PP_0869 at 1008.875 kb on + strand, within PP_0869 at 1008.876 kb on - strand, within PP_0869 at 1008.876 kb on - strand, within PP_0869 at 1009.081 kb on + strand, within PP_0869 at 1009.111 kb on + strand, within PP_0869 at 1009.111 kb on + strand, within PP_0869 at 1009.403 kb on + strand at 1009.404 kb on - strand at 1009.404 kb on - strand at 1009.605 kb on + strand, within PP_0870 at 1009.831 kb on + strand, within PP_0870 at 1009.831 kb on + strand, within PP_0870 at 1009.832 kb on - strand, within PP_0870 at 1009.832 kb on - strand, within PP_0870 at 1009.926 kb on + strand, within PP_0870 at 1009.926 kb on + strand, within PP_0870 at 1009.927 kb on - strand, within PP_0870 at 1009.927 kb on - strand, within PP_0870 at 1009.927 kb on - strand, within PP_0870 at 1009.927 kb on - strand, within PP_0870 at 1009.927 kb on - strand, within PP_0870 at 1009.998 kb on + strand, within PP_0870 at 1009.999 kb on - strand, within PP_0870 at 1010.020 kb on + strand, within PP_0870 at 1010.020 kb on + strand, within PP_0870 at 1010.021 kb on - strand, within PP_0870 at 1010.041 kb on - strand, within PP_0870 at 1010.065 kb on - strand, within PP_0870 at 1010.167 kb on - strand, within PP_0870 at 1010.321 kb on - strand at 1010.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 1,007,745 - PP_0868 0.13 +0.8 1,007,745 - PP_0868 0.13 +0.2 1,007,745 - PP_0868 0.13 -1.9 1,007,858 + PP_0868 0.23 +0.2 1,007,858 + PP_0868 0.23 -0.5 1,008,170 - PP_0868 0.50 -0.4 1,008,353 + PP_0868 0.66 -1.2 1,008,447 + PP_0868 0.74 -0.5 1,008,448 - PP_0868 0.74 -0.2 1,008,448 - PP_0868 0.74 -0.7 1,008,448 - PP_0868 0.74 -0.3 1,008,500 + PP_0868 0.79 -0.1 1,008,501 - PP_0868 0.79 +2.3 1,008,522 + PP_0868 0.80 -1.8 1,008,828 + PP_0869 0.13 -1.4 1,008,829 - PP_0869 0.13 -0.9 1,008,875 + PP_0869 0.20 -1.2 1,008,876 - PP_0869 0.20 -0.5 1,008,876 - PP_0869 0.20 -0.2 1,009,081 + PP_0869 0.52 -1.1 1,009,111 + PP_0869 0.56 -0.6 1,009,111 + PP_0869 0.56 +0.1 1,009,403 + +0.1 1,009,404 - -0.0 1,009,404 - -0.6 1,009,605 + PP_0870 0.22 -0.1 1,009,831 + PP_0870 0.46 -1.0 1,009,831 + PP_0870 0.46 +0.0 1,009,832 - PP_0870 0.46 -1.2 1,009,832 - PP_0870 0.46 -0.3 1,009,926 + PP_0870 0.56 -2.9 1,009,926 + PP_0870 0.56 +2.1 1,009,927 - PP_0870 0.57 -0.2 1,009,927 - PP_0870 0.57 -1.4 1,009,927 - PP_0870 0.57 -0.5 1,009,927 - PP_0870 0.57 +0.5 1,009,927 - PP_0870 0.57 +0.1 1,009,998 + PP_0870 0.64 -0.3 1,009,999 - PP_0870 0.64 +0.6 1,010,020 + PP_0870 0.66 +0.5 1,010,020 + PP_0870 0.66 -0.4 1,010,021 - PP_0870 0.67 +0.4 1,010,041 - PP_0870 0.69 +0.2 1,010,065 - PP_0870 0.71 -0.3 1,010,167 - PP_0870 0.82 -0.7 1,010,321 - +1.0 1,010,321 - +0.0
Or see this region's nucleotide sequence