Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5458 and PP_0800 are separated by 190 nucleotides PP_0800 and PP_0801 overlap by 22 nucleotides PP_0801 and PP_0802 are separated by 88 nucleotides
PP_5458: PP_5458 - conserved exported protein of unknown function, at 919,305 to 919,529
_5458
PP_0800: PP_0800 - conserved protein of unknown function, at 919,720 to 920,472
_0800
PP_0801: PP_0801 - conserved exported protein of unknown function, at 920,451 to 920,762
_0801
PP_0802: PP_0802 - putative chemotaxis protein, at 920,851 to 921,750
_0802
Position (kb) 920
921 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 919.518 kb on - strand at 919.518 kb on - strand at 919.537 kb on + strand at 919.559 kb on + strand at 919.559 kb on + strand at 919.559 kb on + strand at 919.575 kb on - strand at 919.581 kb on - strand at 919.581 kb on - strand at 919.688 kb on - strand at 919.739 kb on + strand at 919.739 kb on + strand at 919.739 kb on + strand at 919.740 kb on - strand at 919.846 kb on + strand, within PP_0800 at 919.846 kb on + strand, within PP_0800 at 919.847 kb on - strand, within PP_0800 at 919.889 kb on + strand, within PP_0800 at 919.890 kb on - strand, within PP_0800 at 919.890 kb on - strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.156 kb on + strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.157 kb on - strand, within PP_0800 at 920.335 kb on + strand, within PP_0800 at 920.335 kb on + strand, within PP_0800 at 920.407 kb on - strand at 920.428 kb on - strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.440 kb on + strand at 920.441 kb on - strand at 920.441 kb on - strand at 920.507 kb on - strand, within PP_0801 at 920.801 kb on - strand at 920.847 kb on + strand at 920.847 kb on + strand at 920.847 kb on + strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.850 kb on - strand at 920.925 kb on + strand at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.003 kb on - strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.319 kb on + strand, within PP_0802 at 921.460 kb on + strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.602 kb on - strand, within PP_0802 at 921.602 kb on - strand, within PP_0802
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 919,518 - +0.3 919,518 - -1.5 919,537 + -0.1 919,559 + -1.9 919,559 + -0.2 919,559 + +1.2 919,575 - -1.3 919,581 - -0.1 919,581 - -0.1 919,688 - -0.2 919,739 + -0.1 919,739 + -0.5 919,739 + -2.1 919,740 - -0.1 919,846 + PP_0800 0.17 -0.7 919,846 + PP_0800 0.17 -0.2 919,847 - PP_0800 0.17 -0.8 919,889 + PP_0800 0.22 -0.3 919,890 - PP_0800 0.23 +0.5 919,890 - PP_0800 0.23 +0.6 920,156 + PP_0800 0.58 -0.5 920,156 + PP_0800 0.58 +0.2 920,156 + PP_0800 0.58 -0.5 920,156 + PP_0800 0.58 +0.8 920,156 + PP_0800 0.58 -3.5 920,156 + PP_0800 0.58 -2.2 920,157 - PP_0800 0.58 -1.4 920,157 - PP_0800 0.58 -0.3 920,157 - PP_0800 0.58 -0.5 920,157 - PP_0800 0.58 +0.7 920,335 + PP_0800 0.82 +1.7 920,335 + PP_0800 0.82 +0.4 920,407 - -0.9 920,428 - +0.2 920,440 + -0.0 920,440 + -0.6 920,440 + +0.0 920,440 + +0.5 920,441 - -0.2 920,441 - +1.2 920,507 - PP_0801 0.18 -0.7 920,801 - +1.1 920,847 + -0.4 920,847 + +0.8 920,847 + -0.8 920,848 - -0.1 920,848 - -0.5 920,848 - -0.4 920,849 + -0.1 920,849 + -0.6 920,849 + -0.5 920,849 + -0.8 920,849 + +0.3 920,849 + -0.5 920,850 - +0.4 920,925 + +0.7 921,002 + PP_0802 0.17 +2.3 921,002 + PP_0802 0.17 -0.8 921,002 + PP_0802 0.17 -0.1 921,002 + PP_0802 0.17 -1.6 921,003 - PP_0802 0.17 +0.4 921,237 + PP_0802 0.43 -0.2 921,237 + PP_0802 0.43 -1.4 921,319 + PP_0802 0.52 -0.6 921,460 + PP_0802 0.68 +1.5 921,461 - PP_0802 0.68 -0.3 921,461 - PP_0802 0.68 +2.4 921,601 + PP_0802 0.83 +0.3 921,601 + PP_0802 0.83 +0.9 921,602 - PP_0802 0.83 -0.3 921,602 - PP_0802 0.83 -0.6
Or see this region's nucleotide sequence