Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0768 and PP_0769 overlap by 4 nucleotides PP_0769 and PP_0770 are separated by 193 nucleotides PP_0770 and PP_0771 overlap by 4 nucleotides PP_0771 and PP_0772 are separated by 18 nucleotides
PP_0768: PP_0768 - Response regulator/TPR domain protein, at 886,967 to 888,574
_0768
PP_0769: PP_0769 - Sensor histidine kinase, at 888,571 to 889,263
_0769
PP_0770: PP_0770 - PemI-like protein, at 889,457 to 889,711
_0770
PP_0771: PP_0771 - mRNA interferase, at 889,708 to 890,055
_0771
PP_0772: PP_0772 - Metallo-beta-lactamase family protein, at 890,074 to 890,718
_0772
Position (kb) 889
890 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 888.530 kb on + strand at 888.779 kb on - strand, within PP_0769 at 888.779 kb on - strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.806 kb on + strand, within PP_0769 at 888.817 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.869 kb on + strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 888.870 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.103 kb on - strand, within PP_0769 at 889.151 kb on - strand, within PP_0769 at 889.211 kb on + strand at 889.211 kb on + strand at 889.211 kb on + strand at 889.257 kb on - strand at 889.278 kb on + strand at 889.278 kb on + strand at 889.324 kb on + strand at 889.324 kb on + strand at 889.382 kb on + strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.383 kb on - strand at 889.387 kb on - strand at 889.430 kb on - strand at 889.430 kb on - strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.432 kb on + strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.433 kb on - strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.436 kb on + strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.437 kb on - strand at 889.440 kb on + strand at 889.441 kb on - strand at 889.452 kb on + strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.453 kb on - strand at 889.515 kb on + strand, within PP_0770 at 889.610 kb on + strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.611 kb on - strand, within PP_0770 at 889.805 kb on + strand, within PP_0771 at 889.806 kb on - strand, within PP_0771 at 889.810 kb on - strand, within PP_0771 at 889.844 kb on + strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.845 kb on - strand, within PP_0771 at 889.991 kb on + strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 889.992 kb on - strand, within PP_0771 at 890.067 kb on - strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.079 kb on + strand at 890.080 kb on - strand at 890.080 kb on - strand at 890.392 kb on + strand, within PP_0772 at 890.392 kb on + strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.393 kb on - strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.469 kb on + strand, within PP_0772 at 890.470 kb on - strand, within PP_0772 at 890.470 kb on - strand, within PP_0772
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 888,530 + +1.4 888,779 - PP_0769 0.30 -0.1 888,779 - PP_0769 0.30 +0.2 888,806 + PP_0769 0.34 +0.5 888,806 + PP_0769 0.34 +1.3 888,817 + PP_0769 0.35 -0.6 888,869 + PP_0769 0.43 -1.7 888,869 + PP_0769 0.43 -0.0 888,870 - PP_0769 0.43 +0.4 888,870 - PP_0769 0.43 -0.3 889,103 - PP_0769 0.77 +0.2 889,103 - PP_0769 0.77 -0.4 889,103 - PP_0769 0.77 +0.6 889,151 - PP_0769 0.84 -0.2 889,211 + +0.4 889,211 + +0.2 889,211 + +0.7 889,257 - -0.5 889,278 + +0.1 889,278 + +0.5 889,324 + +0.3 889,324 + -0.8 889,382 + -0.0 889,383 - -1.3 889,383 - -0.7 889,383 - -0.4 889,383 - +0.7 889,387 - +0.0 889,430 - -3.2 889,430 - +0.8 889,432 + -0.3 889,432 + -1.6 889,432 + +0.3 889,432 + -0.1 889,432 + -0.1 889,432 + -0.5 889,433 - +0.3 889,433 - +0.5 889,433 - -0.1 889,433 - -0.7 889,433 - -1.6 889,433 - +0.2 889,433 - -0.4 889,433 - +1.4 889,433 - -0.6 889,436 + +2.1 889,436 + +0.9 889,436 + -1.4 889,436 + -0.2 889,436 + -1.5 889,436 + +1.0 889,436 + +0.5 889,437 - -1.0 889,437 - -1.9 889,437 - +0.2 889,437 - -0.6 889,437 - +0.1 889,437 - +0.1 889,437 - -0.8 889,437 - +0.6 889,437 - -0.4 889,437 - +0.2 889,440 + -0.2 889,441 - -0.9 889,452 + +0.6 889,453 - +0.0 889,453 - +2.5 889,453 - -0.5 889,515 + PP_0770 0.23 +2.7 889,610 + PP_0770 0.60 +0.1 889,611 - PP_0770 0.60 +0.4 889,611 - PP_0770 0.60 +0.8 889,805 + PP_0771 0.28 -3.1 889,806 - PP_0771 0.28 +1.1 889,810 - PP_0771 0.29 +0.4 889,844 + PP_0771 0.39 +0.1 889,845 - PP_0771 0.39 -0.8 889,845 - PP_0771 0.39 -0.2 889,991 + PP_0771 0.81 +0.6 889,992 - PP_0771 0.82 +0.5 889,992 - PP_0771 0.82 -0.4 890,067 - +0.8 890,079 + +1.7 890,079 + +1.5 890,079 + +0.3 890,079 + -0.1 890,080 - +0.1 890,080 - +0.2 890,392 + PP_0772 0.49 +0.5 890,392 + PP_0772 0.49 +0.7 890,393 - PP_0772 0.49 +0.3 890,393 - PP_0772 0.49 -0.3 890,393 - PP_0772 0.49 -0.3 890,469 + PP_0772 0.61 +2.2 890,469 + PP_0772 0.61 -2.6 890,470 - PP_0772 0.61 -0.5 890,470 - PP_0772 0.61 +0.5
Or see this region's nucleotide sequence