Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4912 and PP_4913 are separated by 13 nucleotides PP_4913 and PP_4914 overlap by 4 nucleotides PP_4914 and PP_4915 overlap by 43 nucleotides
PP_4912: PP_4912 - DNA topoisomerase IV subunit A, at 5,583,473 to 5,585,731
_4912
PP_4913: PP_4913 - conserved protein of unknown function, at 5,585,745 to 5,586,263
_4913
PP_4914: PP_4914 - putative phytase-like protein, at 5,586,260 to 5,587,285
_4914
PP_4915: PP_4915 - DNA topoisomerase IV subunit B, at 5,587,243 to 5,589,147
_4915
Position (kb)
5586
5587
5588 Strain fitness (log2 ratio)
-2
-1
0
1 at 5585.747 kb on - strand at 5585.826 kb on + strand, within PP_4913 at 5585.826 kb on + strand, within PP_4913 at 5585.827 kb on - strand, within PP_4913 at 5585.866 kb on - strand, within PP_4913 at 5585.949 kb on + strand, within PP_4913 at 5585.950 kb on - strand, within PP_4913 at 5586.089 kb on + strand, within PP_4913 at 5586.089 kb on + strand, within PP_4913 at 5586.228 kb on + strand at 5586.229 kb on - strand at 5586.511 kb on + strand, within PP_4914 at 5586.511 kb on + strand, within PP_4914 at 5586.910 kb on + strand, within PP_4914 at 5586.942 kb on + strand, within PP_4914 at 5587.015 kb on + strand, within PP_4914 at 5587.016 kb on - strand, within PP_4914 at 5587.016 kb on - strand, within PP_4914 at 5587.063 kb on + strand, within PP_4914 at 5587.064 kb on - strand, within PP_4914 at 5587.232 kb on + strand at 5587.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,585,747 - -1.0 5,585,826 + PP_4913 0.16 -0.4 5,585,826 + PP_4913 0.16 -0.7 5,585,827 - PP_4913 0.16 -1.5 5,585,866 - PP_4913 0.23 +0.5 5,585,949 + PP_4913 0.39 +0.3 5,585,950 - PP_4913 0.39 -1.2 5,586,089 + PP_4913 0.66 +1.0 5,586,089 + PP_4913 0.66 -0.1 5,586,228 + +0.4 5,586,229 - -0.5 5,586,511 + PP_4914 0.24 -1.9 5,586,511 + PP_4914 0.24 -0.4 5,586,910 + PP_4914 0.63 -0.2 5,586,942 + PP_4914 0.66 -0.6 5,587,015 + PP_4914 0.74 -0.6 5,587,016 - PP_4914 0.74 -1.2 5,587,016 - PP_4914 0.74 +0.8 5,587,063 + PP_4914 0.78 -1.1 5,587,064 - PP_4914 0.78 -0.7 5,587,232 + -0.3 5,587,233 - +0.3
Or see this region's nucleotide sequence