Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4796 and PP_4797 are separated by 216 nucleotides PP_4797 and PP_4798 are separated by 60 nucleotides
PP_4796: PP_4796 - DNA polymerase III, delta subunit, at 5,459,582 to 5,460,619
_4796
PP_4797: PP_4797 - conserved protein of unknown function, at 5,460,836 to 5,461,000
_4797
PP_4798: PP_4798 - putative Membrane-bound lytic murein transglycosylase, at 5,461,061 to 5,462,377
_4798
Position (kb)
5460
5461
5462 Strain fitness (log2 ratio)
-2
-1
0
1 at 5460.633 kb on - strand at 5460.710 kb on + strand at 5460.732 kb on + strand at 5460.738 kb on + strand at 5460.738 kb on + strand at 5460.760 kb on - strand at 5460.760 kb on - strand at 5460.761 kb on + strand at 5460.761 kb on + strand at 5460.761 kb on + strand at 5460.761 kb on + strand at 5460.761 kb on + strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.762 kb on - strand at 5460.797 kb on - strand at 5460.942 kb on - strand, within PP_4797 at 5460.983 kb on + strand, within PP_4797 at 5460.984 kb on - strand at 5461.024 kb on + strand at 5461.024 kb on + strand at 5461.024 kb on + strand at 5461.024 kb on + strand at 5461.025 kb on - strand at 5461.025 kb on - strand at 5461.025 kb on - strand at 5461.025 kb on - strand at 5461.025 kb on - strand at 5461.409 kb on + strand, within PP_4798 at 5461.410 kb on - strand, within PP_4798 at 5461.503 kb on - strand, within PP_4798 at 5461.748 kb on + strand, within PP_4798 at 5461.748 kb on + strand, within PP_4798 at 5461.749 kb on - strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.835 kb on + strand, within PP_4798 at 5461.836 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.885 kb on - strand, within PP_4798 at 5461.999 kb on + strand, within PP_4798
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,460,633 - -0.0 5,460,710 + +0.2 5,460,732 + -1.3 5,460,738 + -0.2 5,460,738 + -0.3 5,460,760 - -0.5 5,460,760 - -0.1 5,460,761 + -0.5 5,460,761 + -0.6 5,460,761 + +1.1 5,460,761 + -0.3 5,460,761 + +1.3 5,460,762 - -0.5 5,460,762 - -0.8 5,460,762 - -0.0 5,460,762 - -0.0 5,460,762 - -1.0 5,460,762 - +0.3 5,460,762 - -0.2 5,460,797 - -0.1 5,460,942 - PP_4797 0.64 -2.5 5,460,983 + PP_4797 0.89 +0.2 5,460,984 - +1.3 5,461,024 + -0.2 5,461,024 + -0.1 5,461,024 + -1.3 5,461,024 + +0.4 5,461,025 - +0.0 5,461,025 - -0.3 5,461,025 - +0.4 5,461,025 - -0.7 5,461,025 - +0.2 5,461,409 + PP_4798 0.26 -1.2 5,461,410 - PP_4798 0.26 -0.4 5,461,503 - PP_4798 0.34 +0.6 5,461,748 + PP_4798 0.52 -1.6 5,461,748 + PP_4798 0.52 -0.5 5,461,749 - PP_4798 0.52 -0.2 5,461,835 + PP_4798 0.59 +0.5 5,461,835 + PP_4798 0.59 +0.0 5,461,835 + PP_4798 0.59 +0.3 5,461,836 - PP_4798 0.59 +0.3 5,461,885 - PP_4798 0.63 +0.3 5,461,885 - PP_4798 0.63 +0.7 5,461,885 - PP_4798 0.63 -0.7 5,461,999 + PP_4798 0.71 -1.2
Or see this region's nucleotide sequence