Strain Fitness in Pseudomonas putida KT2440 around PP_3480

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
3,943,459	+		PP_3478	0.77	+0.3
3,943,460	-		PP_3478	0.77	-0.5
3,943,460	-		PP_3478	0.77	-0.4
3,943,512	+		PP_3478	0.80	-2.4
3,943,604	-		PP_3478	0.85	-0.1
3,943,716	-				+1.0
3,943,768	+				+0.5
3,943,769	-				+0.2
3,943,769	-				+0.0
3,943,939	-				-2.8
3,943,939	-				-1.6
3,943,980	+		PP_5605	0.18	+0.2
3,943,980	+		PP_5605	0.18	+1.2
3,943,980	+		PP_5605	0.18	+1.0
3,943,981	-		PP_5605	0.18	-0.8
3,943,981	-		PP_5605	0.18	-1.6
3,944,243	+		PP_5605	0.70	-0.1
3,944,243	+		PP_5605	0.70	-1.0
3,944,243	+		PP_5605	0.70	-0.1
3,944,243	+		PP_5605	0.70	-0.4
3,944,244	-		PP_5605	0.70	+0.5
3,944,269	+		PP_5605	0.75	-0.9
3,944,269	+		PP_5605	0.75	-0.1
3,944,270	-		PP_5605	0.75	-0.4
3,944,412	-				-1.1
3,944,412	-				-0.4
3,944,412	-				-0.7
3,944,520	+		PP_3480	0.22	-1.6
3,944,520	+		PP_3480	0.22	-0.3
3,944,521	-		PP_3480	0.22	-1.2
3,944,610	-		PP_3480	0.38	-0.8
3,944,773	+		PP_3480	0.67	+0.1
3,945,041	+		PP_3481	0.18	+0.2
3,945,041	+		PP_3481	0.18	+0.5
3,945,081	+		PP_3481	0.25	-3.1
3,945,599	-		PP_3482	0.14	+0.9
3,945,635	+		PP_3482	0.18	-0.6
3,945,727	-		PP_3482	0.29	+0.4
3,945,727	-		PP_3482	0.29	-0.1
3,945,727	-		PP_3482	0.29	+0.7
3,945,727	-		PP_3482	0.29	-0.1
3,945,796	+				+1.1
3,945,796	+		PP_3482	0.38	+0.4
3,945,796	+		PP_3482	0.38	-0.2
3,945,796	+		PP_3482	0.38	+0.3
3,945,796	+		PP_3482	0.38	+0.1
3,945,796	+		PP_3482	0.38	+1.0
3,945,797	-		PP_3482	0.38	+0.3
3,945,797	-		PP_3482	0.38	+1.2
3,945,797	-		PP_3482	0.38	-0.7
3,945,797	-		PP_3482	0.38	+0.4
3,945,797	-		PP_3482	0.38	+0.7
3,945,811	+		PP_3482	0.40	-1.6
3,945,811	+		PP_3482	0.40	-0.3
3,945,812	-		PP_3482	0.40	-0.3
3,945,948	+		PP_3482	0.57	+0.2