Strain Fitness in Pseudomonas putida KT2440 around PP_3475

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
3,939,696	+		PP_3472	0.85	-0.3
3,939,696	+		PP_3472	0.85	+0.2
3,939,696	+		PP_3472	0.85	-0.0
3,939,696	+		PP_3472	0.85	+0.8
3,939,697	-		PP_3472	0.85	+0.6
3,939,700	-		PP_3472	0.85	-0.2
3,939,700	-		PP_3472	0.85	+0.4
3,939,845	+				-2.7
3,939,845	+				+0.2
3,939,846	-				-1.3
3,939,846	-				-2.5
3,939,846	-				-2.9
3,940,077	-		PP_3473	0.52	-0.9
3,940,195	+		PP_3473	0.81	-0.1
3,940,195	+		PP_3473	0.81	+1.0
3,940,195	+		PP_3473	0.81	-0.3
3,940,195	+		PP_3473	0.81	-2.8
3,940,196	-		PP_3473	0.81	-0.0
3,940,196	-		PP_3473	0.81	+0.7
3,940,196	-		PP_3473	0.81	-0.9
3,940,274	+				+0.7
3,940,274	+				+0.7
3,940,275	-				-1.4
3,940,275	-				-0.7
3,940,275	-				+0.6
3,940,275	-				-1.1
3,940,275	-				-0.1
3,940,275	-				+1.6
3,940,307	+				-0.8
3,940,390	+		PP_3474	0.30	-0.6
3,940,391	-		PP_3474	0.30	-0.9
3,940,391	-		PP_3474	0.30	-3.2
3,940,479	-		PP_3474	0.52	-0.5
3,940,531	-		PP_3474	0.65	+0.2
3,940,531	-		PP_3474	0.65	+0.2
3,940,531	-		PP_3474	0.65	+0.0
3,940,531	-		PP_3474	0.65	-2.7
3,940,536	+		PP_3474	0.66	+0.0
3,940,758	-		PP_3475	0.17	+2.2
3,940,835	+		PP_3475	0.31	-0.5
3,940,907	+		PP_3475	0.45	+0.5
3,940,954	+		PP_3475	0.54	+0.2
3,940,980	-		PP_3475	0.59	-0.3
3,941,007	-		PP_3475	0.64	-0.3
3,941,007	-		PP_3475	0.64	+2.2
3,941,009	+		PP_3475	0.64	+1.6
3,941,009	+		PP_3475	0.64	-0.1
3,941,009	+		PP_3475	0.64	+0.3
3,941,031	-		PP_3475	0.68	+0.4
3,941,150	+				+0.1
3,941,150	+				-0.8
3,941,150	+				-0.1
3,941,151	-				-0.6
3,941,151	-				-0.2
3,941,154	+				-0.5
3,941,172	+				-0.5
3,941,172	+				+0.5
3,941,172	+				+1.1
3,941,173	-				+1.0
3,941,173	-				+0.0
3,941,322	+		PP_3476	0.39	+0.9
3,941,376	+		PP_3476	0.53	-0.3
3,941,376	+		PP_3476	0.53	+1.1
3,941,376	+		PP_3476	0.53	-1.3
3,941,376	+		PP_3476	0.53	-2.1
3,941,376	+		PP_3476	0.53	-0.2
3,941,377	-		PP_3476	0.53	-1.1
3,941,380	-		PP_3476	0.54	-0.4
3,941,458	-				+0.7
3,941,603	+		PP_3477	0.11	+1.2
3,941,603	+		PP_3477	0.11	-0.5
3,941,733	-		PP_3477	0.38	-2.1
3,941,733	-		PP_3477	0.38	-0.5
3,941,776	+		PP_3477	0.47	-0.6
3,941,811	+		PP_3477	0.54	+0.2
3,941,855	+		PP_3477	0.63	-0.0
3,941,882	+		PP_3477	0.69	+1.1
3,941,883	-		PP_3477	0.69	-1.3
3,941,920	+		PP_3477	0.77	-0.5
3,941,921	-		PP_3477	0.77	+0.2
3,942,104	+				+2.3