Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3074 and PP_3075 are separated by 225 nucleotides PP_3075 and PP_3076 overlap by 4 nucleotides
PP_3074: PP_3074 - D-beta-hydroxybutyrate permease, at 3,457,299 to 3,458,690
_3074
PP_3075: PP_3075 - putative transcriptional regulator, at 3,458,916 to 3,460,361
_3075
PP_3076: PP_3076 - putative ABC transporter permease protein, at 3,460,358 to 3,461,374
_3076
Position (kb)
3458
3459
3460
3461 Strain fitness (log2 ratio)
-1
0
1 at 3458.102 kb on - strand, within PP_3074 at 3458.321 kb on - strand, within PP_3074 at 3458.359 kb on + strand, within PP_3074 at 3458.363 kb on + strand, within PP_3074 at 3458.363 kb on + strand, within PP_3074 at 3458.364 kb on - strand, within PP_3074 at 3458.364 kb on - strand, within PP_3074 at 3458.382 kb on + strand, within PP_3074 at 3458.382 kb on + strand, within PP_3074 at 3458.507 kb on - strand, within PP_3074 at 3458.507 kb on - strand, within PP_3074 at 3458.623 kb on - strand at 3458.623 kb on - strand at 3458.759 kb on - strand at 3458.838 kb on + strand at 3459.059 kb on - strand at 3459.267 kb on + strand, within PP_3075 at 3459.268 kb on - strand, within PP_3075 at 3459.346 kb on + strand, within PP_3075 at 3459.349 kb on + strand, within PP_3075 at 3459.349 kb on + strand, within PP_3075 at 3459.841 kb on + strand, within PP_3075 at 3460.320 kb on + strand at 3460.320 kb on + strand at 3460.320 kb on + strand at 3460.321 kb on - strand at 3460.321 kb on - strand at 3460.321 kb on - strand at 3460.321 kb on - strand at 3460.321 kb on - strand at 3460.587 kb on + strand, within PP_3076 at 3460.587 kb on + strand, within PP_3076 at 3460.587 kb on + strand, within PP_3076 at 3460.588 kb on - strand, within PP_3076 at 3460.588 kb on - strand, within PP_3076 at 3460.673 kb on + strand, within PP_3076 at 3460.673 kb on + strand, within PP_3076 at 3460.674 kb on - strand, within PP_3076 at 3460.674 kb on - strand, within PP_3076 at 3460.677 kb on + strand, within PP_3076 at 3460.678 kb on - strand, within PP_3076 at 3460.678 kb on - strand, within PP_3076 at 3460.678 kb on - strand, within PP_3076 at 3460.678 kb on - strand, within PP_3076 at 3460.809 kb on + strand, within PP_3076 at 3460.809 kb on + strand, within PP_3076 at 3460.809 kb on + strand, within PP_3076 at 3460.810 kb on - strand, within PP_3076 at 3460.810 kb on - strand, within PP_3076 at 3460.833 kb on - strand, within PP_3076 at 3460.833 kb on - strand, within PP_3076 at 3460.833 kb on - strand, within PP_3076 at 3460.878 kb on + strand, within PP_3076 at 3461.062 kb on + strand, within PP_3076 at 3461.062 kb on + strand, within PP_3076 at 3461.063 kb on - strand, within PP_3076 at 3461.086 kb on + strand, within PP_3076 at 3461.136 kb on + strand, within PP_3076 at 3461.136 kb on + strand, within PP_3076 at 3461.136 kb on + strand, within PP_3076 at 3461.137 kb on - strand, within PP_3076 at 3461.137 kb on - strand, within PP_3076 at 3461.137 kb on - strand, within PP_3076 at 3461.149 kb on - strand, within PP_3076 at 3461.214 kb on + strand, within PP_3076 at 3461.229 kb on + strand, within PP_3076
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,458,102 - PP_3074 0.58 +1.1 3,458,321 - PP_3074 0.73 +0.2 3,458,359 + PP_3074 0.76 +0.3 3,458,363 + PP_3074 0.76 +0.6 3,458,363 + PP_3074 0.76 -0.0 3,458,364 - PP_3074 0.77 +0.0 3,458,364 - PP_3074 0.77 +0.2 3,458,382 + PP_3074 0.78 -1.5 3,458,382 + PP_3074 0.78 -1.7 3,458,507 - PP_3074 0.87 -0.1 3,458,507 - PP_3074 0.87 +0.4 3,458,623 - -1.4 3,458,623 - -1.4 3,458,759 - -1.3 3,458,838 + +1.3 3,459,059 - -1.1 3,459,267 + PP_3075 0.24 +1.4 3,459,268 - PP_3075 0.24 +0.1 3,459,346 + PP_3075 0.30 +0.0 3,459,349 + PP_3075 0.30 +0.6 3,459,349 + PP_3075 0.30 -0.5 3,459,841 + PP_3075 0.64 +1.2 3,460,320 + -1.4 3,460,320 + -0.3 3,460,320 + -0.1 3,460,321 - -0.5 3,460,321 - -0.3 3,460,321 - -0.1 3,460,321 - -0.8 3,460,321 - -1.2 3,460,587 + PP_3076 0.23 -0.3 3,460,587 + PP_3076 0.23 -0.6 3,460,587 + PP_3076 0.23 +0.3 3,460,588 - PP_3076 0.23 -0.6 3,460,588 - PP_3076 0.23 -0.7 3,460,673 + PP_3076 0.31 -1.0 3,460,673 + PP_3076 0.31 -0.4 3,460,674 - PP_3076 0.31 +0.1 3,460,674 - PP_3076 0.31 -0.8 3,460,677 + PP_3076 0.31 -0.1 3,460,678 - PP_3076 0.31 +0.8 3,460,678 - PP_3076 0.31 +0.4 3,460,678 - PP_3076 0.31 -0.1 3,460,678 - PP_3076 0.31 +0.5 3,460,809 + PP_3076 0.44 +0.8 3,460,809 + PP_3076 0.44 -0.2 3,460,809 + PP_3076 0.44 -0.6 3,460,810 - PP_3076 0.44 -0.7 3,460,810 - PP_3076 0.44 +0.1 3,460,833 - PP_3076 0.47 -0.5 3,460,833 - PP_3076 0.47 -0.0 3,460,833 - PP_3076 0.47 +0.0 3,460,878 + PP_3076 0.51 -1.1 3,461,062 + PP_3076 0.69 +0.5 3,461,062 + PP_3076 0.69 +0.7 3,461,063 - PP_3076 0.69 -0.7 3,461,086 + PP_3076 0.72 -0.1 3,461,136 + PP_3076 0.76 -0.1 3,461,136 + PP_3076 0.76 +0.2 3,461,136 + PP_3076 0.76 -0.2 3,461,137 - PP_3076 0.77 +0.5 3,461,137 - PP_3076 0.77 -0.5 3,461,137 - PP_3076 0.77 -0.2 3,461,149 - PP_3076 0.78 +0.1 3,461,214 + PP_3076 0.84 +0.9 3,461,229 + PP_3076 0.86 +0.3
Or see this region's nucleotide sequence