Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2998 and PP_2999 overlap by 4 nucleotides PP_2999 and PP_3000 overlap by 4 nucleotides PP_3000 and PP_3001 overlap by 4 nucleotides
PP_2998: PP_2998 - 2-dehydropantoate 2-reductase, at 3,394,500 to 3,395,447
_2998
PP_2999: PP_2999 - Glyoxalase family protein, at 3,395,444 to 3,395,842
_2999
PP_3000: PP_3000 - MaoC domain protein, at 3,395,839 to 3,396,294
_3000
PP_3001: PP_3001 - CAIB/BAIF family protein, at 3,396,291 to 3,397,523
_3001
Position (kb)
3395
3396 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3394.467 kb on + strand at 3394.468 kb on - strand at 3394.663 kb on + strand, within PP_2998 at 3394.664 kb on - strand, within PP_2998 at 3394.690 kb on + strand, within PP_2998 at 3394.774 kb on + strand, within PP_2998 at 3394.875 kb on + strand, within PP_2998 at 3394.875 kb on + strand, within PP_2998 at 3394.875 kb on + strand, within PP_2998 at 3394.876 kb on - strand, within PP_2998 at 3394.876 kb on - strand, within PP_2998 at 3394.876 kb on - strand, within PP_2998 at 3394.876 kb on - strand, within PP_2998 at 3395.122 kb on - strand, within PP_2998 at 3395.402 kb on + strand at 3395.463 kb on - strand at 3395.580 kb on - strand, within PP_2999 at 3395.902 kb on + strand, within PP_3000 at 3395.902 kb on + strand, within PP_3000 at 3395.903 kb on - strand, within PP_3000 at 3395.903 kb on - strand, within PP_3000 at 3395.933 kb on - strand, within PP_3000 at 3395.981 kb on - strand, within PP_3000 at 3395.981 kb on - strand, within PP_3000 at 3396.287 kb on + strand at 3396.288 kb on - strand at 3396.334 kb on - strand at 3396.357 kb on + strand at 3396.357 kb on + strand at 3396.357 kb on + strand at 3396.586 kb on - strand, within PP_3001 at 3396.657 kb on + strand, within PP_3001 at 3396.658 kb on - strand, within PP_3001 at 3396.788 kb on + strand, within PP_3001 at 3396.807 kb on + strand at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001 at 3396.808 kb on - strand, within PP_3001
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,394,467 + +0.6 3,394,468 - +2.1 3,394,663 + PP_2998 0.17 -0.1 3,394,664 - PP_2998 0.17 +1.0 3,394,690 + PP_2998 0.20 -0.5 3,394,774 + PP_2998 0.29 -1.0 3,394,875 + PP_2998 0.40 +0.2 3,394,875 + PP_2998 0.40 -0.8 3,394,875 + PP_2998 0.40 -0.4 3,394,876 - PP_2998 0.40 -1.0 3,394,876 - PP_2998 0.40 +0.8 3,394,876 - PP_2998 0.40 -0.1 3,394,876 - PP_2998 0.40 -0.6 3,395,122 - PP_2998 0.66 +0.1 3,395,402 + +1.5 3,395,463 - +0.7 3,395,580 - PP_2999 0.34 +1.0 3,395,902 + PP_3000 0.14 +0.1 3,395,902 + PP_3000 0.14 -0.1 3,395,903 - PP_3000 0.14 +0.9 3,395,903 - PP_3000 0.14 +0.0 3,395,933 - PP_3000 0.21 +0.3 3,395,981 - PP_3000 0.31 -3.3 3,395,981 - PP_3000 0.31 +0.2 3,396,287 + -0.4 3,396,288 - -0.1 3,396,334 - -0.8 3,396,357 + +0.2 3,396,357 + +0.2 3,396,357 + +0.2 3,396,586 - PP_3001 0.24 +0.0 3,396,657 + PP_3001 0.30 -0.8 3,396,658 - PP_3001 0.30 -1.2 3,396,788 + PP_3001 0.40 -0.8 3,396,807 + +0.8 3,396,808 - PP_3001 0.42 -0.3 3,396,808 - PP_3001 0.42 -0.9 3,396,808 - PP_3001 0.42 +0.2 3,396,808 - PP_3001 0.42 +0.1
Or see this region's nucleotide sequence