Strain Fitness in Pseudomonas putida KT2440 around PP_2054

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
2,336,178	+		PP_2052	0.87	+1.9
2,336,197	+		PP_2052	0.88	-1.6
2,336,197	+		PP_2052	0.88	+0.3
2,336,198	-		PP_2052	0.88	-0.1
2,336,198	-		PP_2052	0.88	+0.7
2,336,239	+				+0.1
2,336,589	-		PP_5500	0.26	+0.1
2,336,589	-		PP_5500	0.26	-0.8
2,336,711	+		PP_5500	0.86	+0.6
2,336,712	-		PP_5500	0.86	-1.6
2,336,721	+				-0.4
2,336,721	+				-1.1
2,336,722	-				+0.1
2,336,722	-				-0.3
2,336,833	-				+0.7
2,336,880	+				+0.7
2,336,880	+				+0.3
2,336,950	+		PP_2053	0.33	+3.4
2,336,951	-		PP_2053	0.33	+0.1
2,336,951	-		PP_2053	0.33	+0.1
2,336,951	-		PP_2053	0.33	-0.2
2,336,951	-		PP_2053	0.33	-1.7
2,336,951	-		PP_2053	0.33	+0.1
2,337,073	-		PP_2053	0.74	-0.2
2,337,107	+		PP_2053	0.86	+1.0
2,337,107	+		PP_2053	0.86	+0.3
2,337,107	+		PP_2053	0.86	-1.4
2,337,138	-				+0.3
2,337,245	-				+1.0
2,337,255	+				+0.3
2,337,255	+				-1.0
2,337,400	+		PP_2054	0.26	-0.6
2,337,559	-		PP_2054	0.44	-0.6
2,337,719	-		PP_2054	0.62	-0.9
2,337,746	-		PP_2054	0.65	-1.1
2,338,055	-				+0.2
2,338,140	+				-0.2
2,338,227	+				-0.4
2,338,227	+				-1.0
2,338,227	+				+0.4
2,338,536	+		PP_2055	0.30	+0.0
2,338,537	-		PP_2055	0.30	+0.2
2,338,982	+		PP_2055	0.72	+0.8
2,339,006	+		PP_2055	0.74	-0.6
2,339,006	+		PP_2055	0.74	+1.9
2,339,007	-		PP_2055	0.74	+0.4