Strain Fitness in Pseudomonas putida KT2440 around PP_1876

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
2,099,102	+		PP_1875	0.67	-1.9
2,099,309	+		PP_1875	0.75	+0.8
2,099,309	+		PP_1875	0.75	+0.0
2,099,310	-		PP_1875	0.75	+0.5
2,099,310	-		PP_1875	0.75	-0.3
2,099,310	-		PP_1875	0.75	+0.2
2,099,426	+		PP_1875	0.80	-0.4
2,099,426	+		PP_1875	0.80	+0.1
2,099,427	-		PP_1875	0.80	+0.7
2,099,441	+		PP_1875	0.81	+0.2
2,099,441	+		PP_1875	0.81	-0.5
2,099,441	+		PP_1875	0.81	-0.7
2,099,441	+		PP_1875	0.81	-0.3
2,099,442	-		PP_1875	0.81	-0.2
2,099,442	-		PP_1875	0.81	+0.6
2,099,442	-		PP_1875	0.81	-0.1
2,099,442	-		PP_1875	0.81	+0.7
2,099,466	-		PP_1875	0.82	+0.2
2,099,657	+				+0.5
2,099,657	+				+0.3
2,099,657	+				-0.5
2,099,658	-				-0.1
2,099,658	-				+2.3
2,099,816	+				+1.6
2,099,816	+				-0.8
2,099,816	+				-0.7
2,099,816	+				-0.4
2,099,817	-				+0.3
2,099,817	-				-0.3
2,099,842	-				-0.4
2,099,993	+				-0.4
2,099,993	+				-1.0
2,099,994	-				+0.4
2,100,000	+				-1.2
2,100,000	+				-0.5
2,100,001	-				+0.4
2,100,083	+				+0.7
2,100,137	+				+0.5
2,100,565	+		PP_1876	0.53	+2.7
2,100,629	-		PP_1876	0.60	-0.4
2,100,775	+		PP_1876	0.77	+0.4
2,100,775	+		PP_1876	0.77	-0.3
2,100,915	+				-1.1
2,100,915	+				+0.4
2,100,979	+				-0.1
2,100,979	+				+0.5
2,100,979	+				+0.1
2,101,021	+				-0.5
2,101,021	+				-0.5
2,101,021	+				-0.5
2,101,022	-				-0.4
2,101,022	-				-0.6
2,101,022	-				+0.5
2,101,023	+				-0.1
2,101,023	+				+2.0
2,101,024	-				+0.8
2,101,108	+		PP_1877	0.26	+1.3
2,101,109	-		PP_1877	0.26	+0.4
2,101,111	-		PP_1877	0.27	-0.2
2,101,144	+		PP_1877	0.34	+0.1
2,101,145	-		PP_1877	0.34	-0.2
2,101,145	-		PP_1877	0.34	+0.9
2,101,219	-		PP_1877	0.49	-0.0
2,101,219	-		PP_1877	0.49	-0.5
2,101,274	+		PP_1877	0.60	+0.5
2,101,274	+		PP_1877	0.60	-1.9
2,101,274	+		PP_1877	0.60	+0.4
2,101,274	+		PP_1877	0.60	+0.5
2,101,274	+		PP_1877	0.60	+0.3
2,101,274	+		PP_1877	0.60	-0.1
2,101,275	-		PP_1877	0.61	+0.1
2,101,275	-		PP_1877	0.61	+0.3
2,101,275	-		PP_1877	0.61	-0.0
2,101,275	-		PP_1877	0.61	-1.4
2,101,275	-		PP_1877	0.61	-0.1
2,101,275	-		PP_1877	0.61	+0.3
2,101,275	-		PP_1877	0.61	+0.8
2,101,275	-		PP_1877	0.61	-0.4
2,101,496	+				-0.1
2,101,496	+				+2.5
2,101,509	-				-2.2
2,101,547	+				-0.1
2,101,643	-		PP_1878	0.13	-1.2
2,101,679	+		PP_1878	0.18	-1.4
2,101,679	+		PP_1878	0.18	-1.4
2,101,697	+		PP_1878	0.20	+0.2
2,101,697	+		PP_1878	0.20	-0.6
2,101,697	+		PP_1878	0.20	+0.2
2,101,697	+		PP_1878	0.20	+0.5
2,101,697	+		PP_1878	0.20	-0.4
2,101,698	-		PP_1878	0.21	-0.7
2,101,698	-		PP_1878	0.21	+0.1
2,101,698	-		PP_1878	0.21	+0.1
2,101,698	-		PP_1878	0.21	+0.2
2,101,813	+		PP_1878	0.36	-0.8
2,101,813	+		PP_1878	0.36	+0.3
2,101,814	-		PP_1878	0.36	+0.3
2,101,814	-		PP_1878	0.36	-0.7
2,101,814	-		PP_1878	0.36	+0.0
2,101,814	-		PP_1878	0.36	+1.5
2,101,815	+		PP_1878	0.36	-1.3
2,101,816	-		PP_1878	0.36	-0.5
2,101,833	+		PP_1878	0.38	+1.5
2,101,936	+		PP_1878	0.52	+0.2
2,101,936	+		PP_1878	0.52	+0.7
2,101,937	-		PP_1878	0.52	+1.7