Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1047 and PP_1048 are separated by 3 nucleotides PP_1048 and PP_1049 overlap by 4 nucleotides PP_1049 and PP_1050 are separated by 3 nucleotides PP_1050 and PP_1051 overlap by 43 nucleotides
PP_1047: PP_1047 - putative protein secretion protein for export, at 1,197,691 to 1,199,142
_1047
PP_1048: PP_1048 - putative protein secretion protein for export, at 1,199,146 to 1,200,348
_1048
PP_1049: PP_1049 - putative protein secretion protein for export, at 1,200,345 to 1,200,800
_1049
PP_1050: PP_1050 - Type II secretion pathway protein XcpU, at 1,200,804 to 1,201,235
_1050
PP_1051: PP_1051 - Type II secretion pathway protein XcpV, at 1,201,193 to 1,201,600
_1051
Position (kb)
1199
1200
1201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1198.166 kb on + strand, within PP_1047 at 1198.266 kb on - strand, within PP_1047 at 1198.373 kb on + strand, within PP_1047 at 1198.374 kb on - strand, within PP_1047 at 1198.374 kb on - strand, within PP_1047 at 1198.462 kb on + strand, within PP_1047 at 1198.463 kb on - strand, within PP_1047 at 1198.730 kb on + strand, within PP_1047 at 1198.874 kb on + strand at 1198.874 kb on + strand, within PP_1047 at 1198.874 kb on + strand, within PP_1047 at 1198.875 kb on - strand at 1198.906 kb on + strand, within PP_1047 at 1198.948 kb on - strand, within PP_1047 at 1198.967 kb on - strand, within PP_1047 at 1198.967 kb on - strand at 1198.967 kb on - strand, within PP_1047 at 1199.008 kb on + strand at 1199.008 kb on + strand at 1199.009 kb on - strand at 1199.041 kb on + strand at 1199.042 kb on - strand at 1199.042 kb on - strand at 1199.140 kb on + strand at 1199.155 kb on + strand at 1199.156 kb on - strand at 1199.161 kb on + strand at 1199.524 kb on + strand, within PP_1048 at 1199.525 kb on - strand, within PP_1048 at 1199.644 kb on + strand, within PP_1048 at 1200.359 kb on + strand at 1200.360 kb on - strand at 1200.360 kb on - strand at 1200.360 kb on - strand at 1200.428 kb on + strand, within PP_1049 at 1200.428 kb on + strand, within PP_1049 at 1200.543 kb on + strand, within PP_1049 at 1200.543 kb on + strand, within PP_1049 at 1200.544 kb on - strand at 1200.544 kb on - strand, within PP_1049 at 1200.544 kb on - strand, within PP_1049 at 1200.544 kb on - strand, within PP_1049 at 1200.648 kb on + strand, within PP_1049 at 1200.649 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.688 kb on - strand, within PP_1049 at 1200.730 kb on - strand, within PP_1049 at 1200.798 kb on + strand at 1200.799 kb on - strand at 1201.157 kb on + strand, within PP_1050 at 1201.275 kb on + strand, within PP_1051 at 1201.320 kb on + strand, within PP_1051 at 1201.321 kb on - strand, within PP_1051
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 1,198,166 + PP_1047 0.33 +0.8 1,198,266 - PP_1047 0.40 +0.7 1,198,373 + PP_1047 0.47 +1.2 1,198,374 - PP_1047 0.47 -0.7 1,198,374 - PP_1047 0.47 -0.2 1,198,462 + PP_1047 0.53 -0.4 1,198,463 - PP_1047 0.53 -0.3 1,198,730 + PP_1047 0.72 -0.6 1,198,874 + -0.9 1,198,874 + PP_1047 0.81 -1.6 1,198,874 + PP_1047 0.81 +0.2 1,198,875 - -1.1 1,198,906 + PP_1047 0.84 -0.1 1,198,948 - PP_1047 0.87 +0.4 1,198,967 - PP_1047 0.88 -0.1 1,198,967 - -1.9 1,198,967 - PP_1047 0.88 +1.3 1,199,008 + +0.5 1,199,008 + +0.4 1,199,009 - -0.0 1,199,041 + -0.0 1,199,042 - -0.1 1,199,042 - +0.9 1,199,140 + +0.6 1,199,155 + +0.5 1,199,156 - +0.2 1,199,161 + +0.1 1,199,524 + PP_1048 0.31 +1.3 1,199,525 - PP_1048 0.32 +0.4 1,199,644 + PP_1048 0.41 +0.5 1,200,359 + -0.7 1,200,360 - +0.3 1,200,360 - -0.0 1,200,360 - -0.3 1,200,428 + PP_1049 0.18 -1.7 1,200,428 + PP_1049 0.18 -0.3 1,200,543 + PP_1049 0.43 -0.2 1,200,543 + PP_1049 0.43 +0.1 1,200,544 - +1.8 1,200,544 - PP_1049 0.44 +0.5 1,200,544 - PP_1049 0.44 -1.8 1,200,544 - PP_1049 0.44 -0.0 1,200,648 + PP_1049 0.66 -0.3 1,200,649 - PP_1049 0.67 +0.2 1,200,688 - PP_1049 0.75 -0.7 1,200,688 - PP_1049 0.75 -1.0 1,200,730 - PP_1049 0.84 -0.7 1,200,798 + -0.9 1,200,799 - +0.0 1,201,157 + PP_1050 0.82 +1.1 1,201,275 + PP_1051 0.20 -1.7 1,201,320 + PP_1051 0.31 +0.7 1,201,321 - PP_1051 0.31 +1.3
Or see this region's nucleotide sequence