Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0774 and PP_0775 are separated by 31 nucleotides PP_0775 and PP_0776 overlap by 2 nucleotides PP_0776 and PP_0777 are separated by 145 nucleotides
PP_0774: PP_0774 - Phosphate acetyltransferase, at 891,627 to 893,714
_0774
PP_0775: PP_0775 - conserved protein of unknown function, at 893,746 to 894,048
_0775
PP_0776: PP_0776 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 894,047 to 894,532
_0776
PP_0777: PP_0777 - Glutathione peroxidase, at 894,678 to 895,160
_0777
Position (kb) 893
894
895 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 892.756 kb on + strand, within PP_0774 at 892.756 kb on + strand, within PP_0774 at 892.756 kb on + strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.757 kb on - strand, within PP_0774 at 892.957 kb on - strand, within PP_0774 at 893.020 kb on + strand, within PP_0774 at 893.192 kb on + strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.320 kb on - strand, within PP_0774 at 893.438 kb on - strand, within PP_0774 at 893.600 kb on + strand at 893.600 kb on + strand at 893.731 kb on - strand at 893.731 kb on - strand at 893.761 kb on - strand at 894.004 kb on + strand, within PP_0775 at 894.004 kb on + strand, within PP_0775 at 894.005 kb on - strand, within PP_0775 at 894.019 kb on + strand at 894.083 kb on + strand at 894.083 kb on + strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.084 kb on - strand at 894.085 kb on + strand at 894.086 kb on - strand at 894.166 kb on + strand, within PP_0776 at 894.248 kb on + strand, within PP_0776 at 894.257 kb on + strand, within PP_0776 at 894.544 kb on - strand at 894.544 kb on - strand at 894.624 kb on + strand at 894.624 kb on + strand at 894.801 kb on + strand, within PP_0777 at 894.823 kb on - strand, within PP_0777 at 894.831 kb on + strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.832 kb on - strand, within PP_0777 at 894.934 kb on - strand, within PP_0777 at 894.934 kb on - strand, within PP_0777 at 894.997 kb on - strand, within PP_0777 at 894.997 kb on - strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.040 kb on + strand, within PP_0777 at 895.041 kb on - strand, within PP_0777 at 895.041 kb on - strand, within PP_0777
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 892,756 + PP_0774 0.54 +0.8 892,756 + PP_0774 0.54 -1.0 892,756 + PP_0774 0.54 -0.6 892,757 - PP_0774 0.54 +0.6 892,757 - PP_0774 0.54 -1.1 892,757 - PP_0774 0.54 +0.4 892,757 - PP_0774 0.54 -0.1 892,957 - PP_0774 0.64 -0.5 893,020 + PP_0774 0.67 +0.3 893,192 + PP_0774 0.75 +0.9 893,320 - PP_0774 0.81 -0.3 893,320 - PP_0774 0.81 -0.9 893,320 - PP_0774 0.81 -0.3 893,438 - PP_0774 0.87 +0.4 893,600 + -1.4 893,600 + -0.4 893,731 - +0.4 893,731 - +0.2 893,761 - -0.3 894,004 + PP_0775 0.85 -0.0 894,004 + PP_0775 0.85 +0.7 894,005 - PP_0775 0.85 +1.6 894,019 + -1.9 894,083 + +0.4 894,083 + +0.3 894,084 - -2.5 894,084 - -1.5 894,084 - +0.8 894,085 + -1.5 894,086 - -0.8 894,166 + PP_0776 0.24 -0.7 894,248 + PP_0776 0.41 -0.4 894,257 + PP_0776 0.43 -2.8 894,544 - -0.1 894,544 - +0.0 894,624 + -1.3 894,624 + +0.0 894,801 + PP_0777 0.25 +0.9 894,823 - PP_0777 0.30 +1.3 894,831 + PP_0777 0.32 -1.0 894,832 - PP_0777 0.32 +0.3 894,832 - PP_0777 0.32 -0.4 894,832 - PP_0777 0.32 -0.4 894,934 - PP_0777 0.53 +0.1 894,934 - PP_0777 0.53 +2.3 894,997 - PP_0777 0.66 +0.1 894,997 - PP_0777 0.66 +0.7 895,040 + PP_0777 0.75 -0.4 895,040 + PP_0777 0.75 -0.5 895,040 + PP_0777 0.75 -0.0 895,041 - PP_0777 0.75 -0.0 895,041 - PP_0777 0.75 -0.2
Or see this region's nucleotide sequence