Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0693 and PP_0694 overlap by 4 nucleotides PP_0694 and PP_0695 overlap by 11 nucleotides PP_0695 and PP_0696 are separated by 76 nucleotides PP_0696 and PP_0697 overlap by 4 nucleotides
PP_0693: PP_0693 - conserved protein of unknown function, at 804,498 to 807,329
_0693
PP_0694: PP_0694 - conserved protein of unknown function, at 807,326 to 808,033
_0694
PP_0695: PP_0695 - conserved protein of unknown function, at 808,023 to 809,357
_0695
PP_0696: PP_0696 - conserved protein of unknown function, at 809,434 to 810,309
_0696
PP_0697: PP_0697 - ribosomal-protein-S18-alanine N-acetyltransferase, at 810,306 to 810,761
_0697
Position (kb) 808
809
810 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 807.094 kb on + strand at 807.095 kb on - strand at 807.095 kb on - strand at 807.095 kb on - strand at 807.115 kb on + strand at 807.142 kb on + strand at 807.142 kb on + strand at 807.143 kb on - strand at 807.143 kb on - strand at 807.143 kb on - strand at 807.143 kb on - strand at 807.213 kb on - strand at 807.232 kb on + strand at 807.232 kb on + strand at 807.314 kb on - strand at 807.573 kb on + strand, within PP_0694 at 807.573 kb on + strand, within PP_0694 at 807.573 kb on + strand, within PP_0694 at 807.681 kb on + strand, within PP_0694 at 807.681 kb on + strand, within PP_0694 at 807.849 kb on + strand, within PP_0694 at 807.849 kb on + strand, within PP_0694 at 807.850 kb on - strand, within PP_0694 at 807.853 kb on - strand, within PP_0694 at 807.939 kb on + strand, within PP_0694 at 807.940 kb on - strand, within PP_0694 at 807.940 kb on - strand, within PP_0694 at 808.058 kb on + strand at 808.136 kb on - strand at 808.198 kb on + strand, within PP_0695 at 808.199 kb on - strand, within PP_0695 at 808.199 kb on - strand, within PP_0695 at 808.390 kb on + strand, within PP_0695 at 808.391 kb on - strand, within PP_0695 at 808.396 kb on + strand, within PP_0695 at 808.719 kb on + strand, within PP_0695 at 808.719 kb on + strand, within PP_0695 at 808.719 kb on + strand, within PP_0695 at 808.720 kb on - strand, within PP_0695 at 808.774 kb on + strand, within PP_0695 at 808.775 kb on - strand, within PP_0695 at 808.805 kb on - strand, within PP_0695 at 809.056 kb on + strand, within PP_0695 at 809.152 kb on - strand, within PP_0695 at 809.350 kb on + strand at 809.398 kb on + strand at 809.399 kb on - strand at 809.419 kb on + strand at 809.420 kb on - strand at 809.454 kb on - strand at 809.476 kb on + strand at 809.476 kb on + strand at 809.483 kb on + strand at 809.483 kb on + strand at 809.483 kb on + strand at 809.483 kb on + strand at 809.483 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 807,094 + -0.9 807,095 - +0.3 807,095 - -1.1 807,095 - -0.6 807,115 + -1.2 807,142 + -0.2 807,142 + -1.5 807,143 - -0.1 807,143 - -0.6 807,143 - +0.0 807,143 - -2.3 807,213 - +0.1 807,232 + +0.2 807,232 + +0.1 807,314 - +0.4 807,573 + PP_0694 0.35 -0.9 807,573 + PP_0694 0.35 -0.1 807,573 + PP_0694 0.35 +0.7 807,681 + PP_0694 0.50 +0.0 807,681 + PP_0694 0.50 +0.9 807,849 + PP_0694 0.74 -0.1 807,849 + PP_0694 0.74 +0.8 807,850 - PP_0694 0.74 +0.0 807,853 - PP_0694 0.74 +0.9 807,939 + PP_0694 0.87 +0.5 807,940 - PP_0694 0.87 -0.2 807,940 - PP_0694 0.87 -1.4 808,058 + +0.8 808,136 - +0.8 808,198 + PP_0695 0.13 +0.2 808,199 - PP_0695 0.13 +1.0 808,199 - PP_0695 0.13 -2.9 808,390 + PP_0695 0.27 +0.4 808,391 - PP_0695 0.28 -0.4 808,396 + PP_0695 0.28 -0.5 808,719 + PP_0695 0.52 -0.6 808,719 + PP_0695 0.52 +0.0 808,719 + PP_0695 0.52 -0.1 808,720 - PP_0695 0.52 +1.4 808,774 + PP_0695 0.56 +0.1 808,775 - PP_0695 0.56 +0.2 808,805 - PP_0695 0.59 +0.0 809,056 + PP_0695 0.77 -0.7 809,152 - PP_0695 0.85 +0.4 809,350 + +0.5 809,398 + -0.8 809,399 - -0.4 809,419 + -0.4 809,420 - -0.1 809,454 - -0.6 809,476 + +0.0 809,476 + -0.0 809,483 + +0.3 809,483 + -0.1 809,483 + -0.0 809,483 + -2.1 809,483 + -0.6
Or see this region's nucleotide sequence