Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0260 and PP_0261 are separated by 14 nucleotides PP_0261 and PP_0262 are separated by 82 nucleotides PP_0262 and PP_0263 overlap by 1 nucleotides
PP_0260: PP_0260 - ADP-sugar pyrophosphorylase, at 314,605 to 315,171
_0260
PP_0261: PP_0261 - adenosine-3'(2'),5'-bisphosphate nucleotidase, at 315,186 to 315,986
_0261
PP_0262: PP_0262 - conserved protein of unknown function, at 316,069 to 316,524
_0262
PP_0263: PP_0263 - C4-dicarboxylate transport transcriptional regulatory protein, at 316,524 to 317,930
_0263
Position (kb) 316
317 Strain fitness (log2 ratio) -2
-1
0
1
2 at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.167 kb on + strand at 315.168 kb on - strand at 315.301 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.380 kb on + strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.381 kb on - strand, within PP_0261 at 315.550 kb on - strand, within PP_0261 at 315.616 kb on + strand, within PP_0261 at 315.616 kb on + strand, within PP_0261 at 315.616 kb on + strand, within PP_0261 at 315.617 kb on - strand, within PP_0261 at 315.617 kb on - strand, within PP_0261 at 315.825 kb on - strand, within PP_0261 at 315.920 kb on + strand at 315.927 kb on + strand at 315.927 kb on + strand at 315.927 kb on + strand at 315.946 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.984 kb on + strand at 315.985 kb on - strand at 316.067 kb on + strand at 316.067 kb on + strand at 316.067 kb on + strand at 316.067 kb on + strand at 316.068 kb on - strand at 316.070 kb on + strand at 316.070 kb on + strand at 316.071 kb on - strand at 316.071 kb on - strand at 316.085 kb on + strand at 316.085 kb on + strand at 316.086 kb on - strand at 316.256 kb on + strand, within PP_0262 at 316.284 kb on + strand, within PP_0262 at 316.284 kb on + strand, within PP_0262 at 316.284 kb on + strand, within PP_0262 at 316.284 kb on + strand, within PP_0262 at 316.285 kb on - strand, within PP_0262 at 316.285 kb on - strand, within PP_0262 at 316.285 kb on - strand, within PP_0262 at 316.359 kb on - strand, within PP_0262 at 316.815 kb on + strand, within PP_0263 at 316.815 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.941 kb on + strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.942 kb on - strand, within PP_0263 at 316.974 kb on - strand, within PP_0263 at 316.999 kb on + strand, within PP_0263 at 316.999 kb on + strand, within PP_0263 at 317.002 kb on + strand, within PP_0263 at 317.003 kb on - strand, within PP_0263 at 317.065 kb on - strand, within PP_0263 at 317.151 kb on + strand, within PP_0263 at 317.151 kb on + strand, within PP_0263 at 317.152 kb on - strand, within PP_0263 at 317.152 kb on - strand, within PP_0263 at 317.152 kb on - strand, within PP_0263 at 317.522 kb on + strand, within PP_0263
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 315,167 + -0.8 315,167 + -0.9 315,167 + +0.3 315,167 + +0.5 315,167 + -1.0 315,167 + +0.2 315,167 + -0.6 315,168 - -0.7 315,301 + PP_0261 0.14 -1.0 315,380 + PP_0261 0.24 -0.9 315,380 + PP_0261 0.24 -0.8 315,380 + PP_0261 0.24 -0.7 315,381 - PP_0261 0.24 -1.5 315,381 - PP_0261 0.24 +0.1 315,381 - PP_0261 0.24 +0.5 315,381 - PP_0261 0.24 -0.4 315,550 - PP_0261 0.45 -1.3 315,616 + PP_0261 0.54 -1.9 315,616 + PP_0261 0.54 -1.7 315,616 + PP_0261 0.54 -0.3 315,617 - PP_0261 0.54 -2.2 315,617 - PP_0261 0.54 -0.2 315,825 - PP_0261 0.80 -0.5 315,920 + -1.6 315,927 + -0.1 315,927 + -1.7 315,927 + -1.2 315,946 + -1.8 315,984 + +0.4 315,984 + -0.7 315,984 + +0.4 315,984 + -1.4 315,984 + -1.3 315,984 + +0.6 315,984 + +0.3 315,984 + -0.2 315,985 - -0.5 316,067 + -0.5 316,067 + +0.5 316,067 + -0.5 316,067 + -0.2 316,068 - -0.4 316,070 + -0.6 316,070 + +0.6 316,071 - -0.7 316,071 - +0.1 316,085 + -0.3 316,085 + -1.4 316,086 - -0.0 316,256 + PP_0262 0.41 +2.7 316,284 + PP_0262 0.47 +1.0 316,284 + PP_0262 0.47 -0.1 316,284 + PP_0262 0.47 +0.9 316,284 + PP_0262 0.47 -0.9 316,285 - PP_0262 0.47 +0.1 316,285 - PP_0262 0.47 +0.1 316,285 - PP_0262 0.47 +1.5 316,359 - PP_0262 0.64 +1.2 316,815 + PP_0263 0.21 +0.5 316,815 + PP_0263 0.21 -1.9 316,941 + PP_0263 0.30 -0.9 316,941 + PP_0263 0.30 +0.5 316,941 + PP_0263 0.30 +0.9 316,941 + PP_0263 0.30 +0.3 316,941 + PP_0263 0.30 +0.3 316,941 + PP_0263 0.30 -0.9 316,942 - PP_0263 0.30 -0.1 316,942 - PP_0263 0.30 +0.4 316,942 - PP_0263 0.30 -0.2 316,942 - PP_0263 0.30 -0.2 316,942 - PP_0263 0.30 +0.3 316,942 - PP_0263 0.30 +0.6 316,942 - PP_0263 0.30 -0.6 316,942 - PP_0263 0.30 -0.1 316,942 - PP_0263 0.30 +1.3 316,942 - PP_0263 0.30 -0.8 316,942 - PP_0263 0.30 -0.7 316,974 - PP_0263 0.32 -0.5 316,999 + PP_0263 0.34 +0.1 316,999 + PP_0263 0.34 +2.3 317,002 + PP_0263 0.34 +0.1 317,003 - PP_0263 0.34 +0.4 317,065 - PP_0263 0.38 +0.3 317,151 + PP_0263 0.45 -0.1 317,151 + PP_0263 0.45 +1.0 317,152 - PP_0263 0.45 +0.0 317,152 - PP_0263 0.45 -0.4 317,152 - PP_0263 0.45 -0.6 317,522 + PP_0263 0.71 -0.7
Or see this region's nucleotide sequence