Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0254 and PP_0255 are separated by 56 nucleotides PP_0255 and PP_0256 are separated by 196 nucleotides
PP_0254: PP_0254 - conserved protein of unknown function, at 308,854 to 309,294
_0254
PP_0255: PP_0255 - conserved exported protein of unknown function, at 309,351 to 309,833
_0255
PP_0256: PP_0256 - putative oxidoreductase, at 310,030 to 312,375
_0256
Position (kb) 308
309
310 Strain fitness (log2 ratio) -2
-1
0
1
2 at 307.935 kb on + strand at 307.936 kb on - strand at 308.006 kb on - strand at 308.053 kb on - strand at 308.139 kb on - strand at 308.236 kb on - strand at 308.341 kb on - strand at 308.363 kb on + strand at 308.364 kb on - strand at 308.388 kb on + strand at 308.389 kb on - strand at 308.607 kb on + strand at 308.703 kb on + strand at 308.704 kb on - strand at 308.787 kb on + strand at 308.788 kb on - strand at 308.788 kb on - strand at 309.134 kb on + strand, within PP_0254 at 309.134 kb on + strand, within PP_0254 at 309.135 kb on - strand, within PP_0254 at 309.139 kb on + strand, within PP_0254 at 309.292 kb on + strand at 309.353 kb on - strand at 309.388 kb on + strand at 309.388 kb on + strand at 309.389 kb on - strand at 309.389 kb on - strand at 309.487 kb on + strand, within PP_0255 at 309.487 kb on + strand, within PP_0255 at 309.487 kb on + strand, within PP_0255 at 309.487 kb on + strand, within PP_0255 at 309.488 kb on - strand, within PP_0255 at 309.488 kb on - strand, within PP_0255 at 309.488 kb on - strand, within PP_0255 at 309.488 kb on - strand, within PP_0255 at 309.492 kb on - strand, within PP_0255 at 309.535 kb on + strand, within PP_0255 at 309.535 kb on + strand, within PP_0255 at 309.535 kb on + strand, within PP_0255 at 309.535 kb on + strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.536 kb on - strand, within PP_0255 at 309.539 kb on + strand, within PP_0255 at 309.539 kb on + strand, within PP_0255 at 309.575 kb on + strand, within PP_0255 at 309.823 kb on + strand at 309.823 kb on + strand at 309.824 kb on - strand at 309.824 kb on - strand at 309.824 kb on - strand at 309.847 kb on + strand at 309.847 kb on + strand at 309.848 kb on - strand at 309.848 kb on - strand at 309.874 kb on - strand at 309.919 kb on + strand at 309.942 kb on - strand at 309.946 kb on - strand at 309.985 kb on + strand at 310.102 kb on - strand at 310.148 kb on + strand at 310.151 kb on + strand at 310.151 kb on + strand at 310.152 kb on - strand at 310.174 kb on + strand at 310.174 kb on + strand at 310.194 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 307,935 + +0.6 307,936 - +0.5 308,006 - +1.1 308,053 - +0.3 308,139 - +1.6 308,236 - -0.2 308,341 - -1.0 308,363 + +0.1 308,364 - +0.3 308,388 + -0.1 308,389 - -0.3 308,607 + +0.3 308,703 + +0.6 308,704 - +0.6 308,787 + -2.0 308,788 - +0.5 308,788 - +0.8 309,134 + PP_0254 0.63 +1.0 309,134 + PP_0254 0.63 +1.0 309,135 - PP_0254 0.64 +0.1 309,139 + PP_0254 0.65 -0.8 309,292 + -0.4 309,353 - +0.5 309,388 + -0.1 309,388 + +0.1 309,389 - -0.6 309,389 - +0.4 309,487 + PP_0255 0.28 +0.4 309,487 + PP_0255 0.28 +0.7 309,487 + PP_0255 0.28 -0.4 309,487 + PP_0255 0.28 +0.1 309,488 - PP_0255 0.28 +0.2 309,488 - PP_0255 0.28 +0.3 309,488 - PP_0255 0.28 -0.3 309,488 - PP_0255 0.28 +0.2 309,492 - PP_0255 0.29 -1.1 309,535 + PP_0255 0.38 -2.1 309,535 + PP_0255 0.38 +0.1 309,535 + PP_0255 0.38 -0.1 309,535 + PP_0255 0.38 -0.4 309,536 - PP_0255 0.38 +0.7 309,536 - PP_0255 0.38 +0.4 309,536 - PP_0255 0.38 +0.3 309,536 - PP_0255 0.38 -0.2 309,536 - PP_0255 0.38 +1.6 309,536 - PP_0255 0.38 +0.6 309,536 - PP_0255 0.38 +0.1 309,536 - PP_0255 0.38 +1.3 309,536 - PP_0255 0.38 +0.2 309,539 + PP_0255 0.39 +0.5 309,539 + PP_0255 0.39 +0.3 309,575 + PP_0255 0.46 -0.1 309,823 + +0.2 309,823 + +0.9 309,824 - +0.1 309,824 - +0.0 309,824 - +0.7 309,847 + +0.4 309,847 + -0.5 309,848 - +0.1 309,848 - +2.2 309,874 - -0.1 309,919 + +0.1 309,942 - +0.7 309,946 - +0.7 309,985 + -0.4 310,102 - -0.1 310,148 + +0.1 310,151 + -0.2 310,151 + +0.1 310,152 - -1.7 310,174 + +0.0 310,174 + +0.5 310,194 + +0.5
Or see this region's nucleotide sequence