Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4742 and PP_5711 are separated by 10 nucleotides PP_5711 and PP_4743 are separated by 29 nucleotides
PP_4742: PP_4742 - Type I restriction-modification system, S subunit, at 5,396,989 to 5,398,719
_4742
PP_5711: PP_5711 - protein of unknown function, at 5,398,730 to 5,399,158
_5711
PP_4743: PP_4743 - conserved protein of unknown function, at 5,399,188 to 5,400,624
_4743
Position (kb)
5398
5399
5400 Strain fitness (log2 ratio)
-1
0
1
2 at 5397.740 kb on + strand, within PP_4742 at 5397.749 kb on + strand, within PP_4742 at 5397.749 kb on + strand, within PP_4742 at 5397.981 kb on + strand, within PP_4742 at 5398.145 kb on + strand, within PP_4742 at 5398.145 kb on + strand, within PP_4742 at 5398.146 kb on - strand, within PP_4742 at 5398.192 kb on - strand, within PP_4742 at 5398.256 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.277 kb on + strand, within PP_4742 at 5398.278 kb on - strand, within PP_4742 at 5398.326 kb on + strand, within PP_4742 at 5398.328 kb on + strand, within PP_4742 at 5398.329 kb on - strand, within PP_4742 at 5398.332 kb on - strand, within PP_4742 at 5398.400 kb on + strand, within PP_4742 at 5398.498 kb on + strand, within PP_4742 at 5398.499 kb on - strand, within PP_4742 at 5398.542 kb on + strand, within PP_4742 at 5398.650 kb on - strand at 5398.661 kb on + strand at 5398.760 kb on + strand at 5398.816 kb on - strand, within PP_5711 at 5398.872 kb on + strand, within PP_5711 at 5398.873 kb on - strand, within PP_5711 at 5398.886 kb on + strand, within PP_5711 at 5398.933 kb on + strand, within PP_5711 at 5398.966 kb on + strand, within PP_5711 at 5399.156 kb on + strand at 5399.161 kb on - strand at 5399.323 kb on - strand at 5399.410 kb on - strand, within PP_4743 at 5399.897 kb on + strand, within PP_4743
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,397,740 + PP_4742 0.43 -0.5 5,397,749 + PP_4742 0.44 +0.0 5,397,749 + PP_4742 0.44 -0.5 5,397,981 + PP_4742 0.57 -0.1 5,398,145 + PP_4742 0.67 -1.0 5,398,145 + PP_4742 0.67 -0.8 5,398,146 - PP_4742 0.67 -0.1 5,398,192 - PP_4742 0.69 -0.2 5,398,256 + PP_4742 0.73 +0.0 5,398,277 + PP_4742 0.74 -0.9 5,398,277 + PP_4742 0.74 -0.5 5,398,278 - PP_4742 0.74 +0.3 5,398,326 + PP_4742 0.77 -0.4 5,398,328 + PP_4742 0.77 +0.9 5,398,329 - PP_4742 0.77 -0.1 5,398,332 - PP_4742 0.78 -0.5 5,398,400 + PP_4742 0.82 -0.4 5,398,498 + PP_4742 0.87 +0.3 5,398,499 - PP_4742 0.87 +0.1 5,398,542 + PP_4742 0.90 +1.1 5,398,650 - -1.1 5,398,661 + -0.8 5,398,760 + -0.9 5,398,816 - PP_5711 0.20 +1.9 5,398,872 + PP_5711 0.33 +0.0 5,398,873 - PP_5711 0.33 +0.2 5,398,886 + PP_5711 0.36 -0.3 5,398,933 + PP_5711 0.47 +0.8 5,398,966 + PP_5711 0.55 +0.6 5,399,156 + -0.4 5,399,161 - -0.5 5,399,323 - -0.4 5,399,410 - PP_4743 0.15 +0.9 5,399,897 + PP_4743 0.49 +0.4
Or see this region's nucleotide sequence