Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3260 and PP_3261 are separated by 190 nucleotides PP_3261 and PP_3262 overlap by 4 nucleotides PP_3262 and PP_5595 are separated by 91 nucleotides PP_5595 and PP_3263 are separated by 28 nucleotides PP_3263 and PP_3264 overlap by 1 nucleotides
PP_3260: PP_3260 - DNA ligase D, at 3,693,666 to 3,696,167
_3260
PP_3261: PP_3261 - conserved protein of unknown function, at 3,696,358 to 3,696,759
_3261
PP_3262: PP_3262 - putative Metallothionein, at 3,696,756 to 3,696,980
_3262
PP_5595: PP_5595 - conserved exported protein of unknown function, at 3,697,072 to 3,697,488
_5595
PP_3263: PP_3263 - phospholipid modification enzyme, at 3,697,517 to 3,698,470
_3263
PP_3264: PP_3264 - cardiolipin synthase 2, at 3,698,470 to 3,699,672
_3264
Position (kb)
3697
3698 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3696.146 kb on - strand at 3696.146 kb on - strand at 3696.196 kb on - strand at 3696.196 kb on - strand at 3696.222 kb on + strand at 3696.315 kb on + strand at 3696.315 kb on + strand at 3696.316 kb on - strand at 3696.322 kb on + strand at 3696.322 kb on + strand at 3696.322 kb on + strand at 3696.323 kb on - strand at 3696.834 kb on + strand, within PP_3262 at 3696.835 kb on - strand, within PP_3262 at 3696.988 kb on + strand at 3697.030 kb on + strand at 3697.031 kb on - strand at 3697.143 kb on - strand, within PP_5595 at 3697.143 kb on - strand, within PP_5595 at 3697.143 kb on - strand, within PP_5595 at 3697.145 kb on + strand, within PP_5595 at 3697.145 kb on + strand at 3697.145 kb on + strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.146 kb on - strand, within PP_5595 at 3697.355 kb on + strand, within PP_5595 at 3697.355 kb on + strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.362 kb on - strand, within PP_5595 at 3697.579 kb on - strand at 3697.579 kb on - strand at 3697.611 kb on + strand at 3697.611 kb on + strand at 3697.614 kb on + strand, within PP_3263 at 3697.614 kb on + strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.615 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.777 kb on - strand, within PP_3263 at 3697.941 kb on + strand, within PP_3263 at 3697.991 kb on - strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand, within PP_3263 at 3698.039 kb on + strand at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.059 kb on + strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.060 kb on - strand, within PP_3263 at 3698.061 kb on + strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.062 kb on - strand, within PP_3263 at 3698.196 kb on + strand, within PP_3263 at 3698.219 kb on + strand, within PP_3263 at 3698.234 kb on + strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.240 kb on - strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.271 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.289 kb on + strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.290 kb on - strand, within PP_3263 at 3698.337 kb on + strand, within PP_3263 at 3698.338 kb on - strand, within PP_3263 at 3698.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,696,146 - -0.4 3,696,146 - +0.5 3,696,196 - +0.1 3,696,196 - +0.5 3,696,222 + -0.1 3,696,315 + -0.1 3,696,315 + +0.8 3,696,316 - +0.4 3,696,322 + -0.6 3,696,322 + -1.3 3,696,322 + +0.5 3,696,323 - -0.3 3,696,834 + PP_3262 0.35 +0.7 3,696,835 - PP_3262 0.35 +1.3 3,696,988 + -0.1 3,697,030 + +0.3 3,697,031 - -1.1 3,697,143 - PP_5595 0.17 -0.1 3,697,143 - PP_5595 0.17 -0.4 3,697,143 - PP_5595 0.17 +2.3 3,697,145 + PP_5595 0.18 +0.1 3,697,145 + +0.6 3,697,145 + PP_5595 0.18 -1.8 3,697,146 - PP_5595 0.18 -0.8 3,697,146 - +4.5 3,697,146 - PP_5595 0.18 +1.1 3,697,146 - PP_5595 0.18 +1.5 3,697,146 - PP_5595 0.18 -0.2 3,697,146 - PP_5595 0.18 +0.5 3,697,355 + PP_5595 0.68 -0.7 3,697,355 + PP_5595 0.68 -0.4 3,697,362 - PP_5595 0.70 +3.2 3,697,362 - PP_5595 0.70 +0.8 3,697,362 - PP_5595 0.70 -1.1 3,697,362 - PP_5595 0.70 +1.6 3,697,579 - -0.0 3,697,579 - -0.2 3,697,611 + -0.7 3,697,611 + +0.5 3,697,614 + PP_3263 0.10 -1.7 3,697,614 + PP_3263 0.10 +0.5 3,697,615 - PP_3263 0.10 -0.0 3,697,615 - PP_3263 0.10 +3.4 3,697,777 - PP_3263 0.27 +2.5 3,697,777 - PP_3263 0.27 -0.3 3,697,941 + PP_3263 0.44 -0.4 3,697,991 - PP_3263 0.50 +1.8 3,698,039 + PP_3263 0.55 +0.8 3,698,039 + PP_3263 0.55 -1.0 3,698,039 + -1.1 3,698,059 + PP_3263 0.57 +0.8 3,698,059 + PP_3263 0.57 +0.5 3,698,059 + PP_3263 0.57 -0.5 3,698,060 - PP_3263 0.57 +0.5 3,698,060 - PP_3263 0.57 +0.3 3,698,060 - PP_3263 0.57 +0.6 3,698,060 - PP_3263 0.57 -0.5 3,698,061 + PP_3263 0.57 -0.3 3,698,062 - PP_3263 0.57 -1.0 3,698,062 - PP_3263 0.57 -0.3 3,698,196 + PP_3263 0.71 -0.9 3,698,219 + PP_3263 0.74 -0.6 3,698,234 + PP_3263 0.75 +0.4 3,698,240 - PP_3263 0.76 -0.2 3,698,240 - PP_3263 0.76 +0.6 3,698,271 + PP_3263 0.79 +0.5 3,698,271 + PP_3263 0.79 +0.6 3,698,289 + PP_3263 0.81 +0.7 3,698,289 + PP_3263 0.81 +0.5 3,698,290 - PP_3263 0.81 -0.1 3,698,290 - PP_3263 0.81 +0.4 3,698,337 + PP_3263 0.86 +0.8 3,698,338 - PP_3263 0.86 +0.8 3,698,392 - +0.9
Or see this region's nucleotide sequence