Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5177 and PP_5178 overlap by 4 nucleotides PP_5178 and PP_5179 overlap by 4 nucleotides PP_5179 and PP_5180 are separated by 71 nucleotides
PP_5177: PP_5177 - spermidine/putrescine ABC transporter - permease subunit, at 5,903,851 to 5,904,744
_5177
PP_5178: PP_5178 - spermidine/putrescine ABC transporter - inner membrane subunit, at 5,904,741 to 5,905,661
_5178
PP_5179: PP_5179 - spermidine/putrescine ABC transporter - ATP binding subunit, at 5,905,658 to 5,906,800
_5179
PP_5180: PP_5180 - spermidine/putrescine ABC transporter - periplasmic subunit, at 5,906,872 to 5,907,966
_5180
Position (kb) 5905
5906
5907 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5904.662 kb on + strand at 5904.663 kb on - strand at 5904.663 kb on - strand at 5904.689 kb on + strand at 5904.690 kb on - strand at 5904.773 kb on + strand at 5904.881 kb on + strand, within PP_5178 at 5904.993 kb on + strand, within PP_5178 at 5905.071 kb on - strand, within PP_5178 at 5905.112 kb on - strand, within PP_5178 at 5905.112 kb on - strand, within PP_5178 at 5905.112 kb on - strand, within PP_5178 at 5905.112 kb on - strand, within PP_5178 at 5905.117 kb on + strand, within PP_5178 at 5905.117 kb on + strand at 5905.118 kb on - strand, within PP_5178 at 5905.118 kb on - strand, within PP_5178 at 5905.133 kb on - strand, within PP_5178 at 5905.133 kb on - strand, within PP_5178 at 5905.133 kb on - strand, within PP_5178 at 5905.240 kb on + strand, within PP_5178 at 5905.400 kb on - strand, within PP_5178 at 5905.411 kb on + strand, within PP_5178 at 5905.412 kb on - strand, within PP_5178 at 5905.412 kb on - strand, within PP_5178 at 5905.412 kb on - strand, within PP_5178 at 5905.438 kb on + strand, within PP_5178 at 5905.439 kb on - strand, within PP_5178 at 5905.480 kb on + strand at 5905.481 kb on - strand, within PP_5178 at 5905.481 kb on - strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.486 kb on + strand, within PP_5178 at 5905.487 kb on - strand, within PP_5178 at 5905.487 kb on - strand, within PP_5178 at 5905.487 kb on - strand, within PP_5178 at 5905.487 kb on - strand, within PP_5178 at 5905.497 kb on - strand, within PP_5178 at 5905.498 kb on + strand, within PP_5178 at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.697 kb on + strand at 5905.698 kb on - strand at 5905.698 kb on - strand at 5905.698 kb on - strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.701 kb on + strand at 5905.702 kb on - strand at 5905.702 kb on - strand at 5905.702 kb on - strand at 5905.702 kb on - strand at 5905.702 kb on - strand at 5905.702 kb on - strand at 5905.730 kb on - strand at 5905.792 kb on - strand, within PP_5179 at 5905.792 kb on - strand, within PP_5179 at 5905.905 kb on + strand, within PP_5179 at 5905.905 kb on + strand, within PP_5179 at 5905.906 kb on - strand, within PP_5179 at 5905.906 kb on - strand, within PP_5179 at 5905.953 kb on + strand, within PP_5179 at 5905.953 kb on + strand, within PP_5179 at 5905.953 kb on + strand, within PP_5179 at 5905.953 kb on + strand, within PP_5179 at 5905.954 kb on - strand, within PP_5179 at 5906.068 kb on + strand, within PP_5179 at 5906.069 kb on - strand, within PP_5179 at 5906.079 kb on + strand, within PP_5179 at 5906.079 kb on + strand, within PP_5179 at 5906.079 kb on + strand, within PP_5179 at 5906.080 kb on - strand, within PP_5179 at 5906.080 kb on - strand, within PP_5179 at 5906.368 kb on - strand, within PP_5179 at 5906.494 kb on - strand, within PP_5179 at 5906.494 kb on - strand, within PP_5179 at 5906.494 kb on - strand, within PP_5179 at 5906.527 kb on - strand, within PP_5179 at 5906.778 kb on + strand at 5906.845 kb on + strand at 5906.892 kb on - strand at 5906.978 kb on + strand at 5906.998 kb on + strand, within PP_5180 at 5906.999 kb on - strand, within PP_5180 at 5907.053 kb on + strand, within PP_5180 at 5907.054 kb on - strand, within PP_5180 at 5907.054 kb on - strand, within PP_5180 at 5907.241 kb on + strand, within PP_5180 at 5907.241 kb on + strand, within PP_5180 at 5907.241 kb on + strand, within PP_5180 at 5907.242 kb on - strand, within PP_5180 at 5907.242 kb on - strand, within PP_5180 at 5907.299 kb on + strand, within PP_5180 at 5907.300 kb on - strand, within PP_5180 at 5907.300 kb on - strand, within PP_5180 at 5907.300 kb on - strand, within PP_5180 at 5907.317 kb on + strand, within PP_5180 at 5907.317 kb on + strand, within PP_5180 at 5907.317 kb on + strand, within PP_5180 at 5907.317 kb on + strand, within PP_5180 at 5907.326 kb on + strand, within PP_5180 at 5907.326 kb on + strand, within PP_5180 at 5907.326 kb on + strand, within PP_5180 at 5907.326 kb on + strand, within PP_5180 at 5907.327 kb on - strand, within PP_5180 at 5907.365 kb on + strand, within PP_5180 at 5907.365 kb on + strand, within PP_5180 at 5907.366 kb on - strand, within PP_5180 at 5907.404 kb on + strand, within PP_5180 at 5907.404 kb on + strand, within PP_5180 at 5907.404 kb on + strand, within PP_5180 at 5907.405 kb on - strand, within PP_5180 at 5907.405 kb on - strand, within PP_5180 at 5907.405 kb on - strand, within PP_5180 at 5907.405 kb on - strand, within PP_5180 at 5907.405 kb on - strand, within PP_5180 at 5907.554 kb on + strand, within PP_5180 at 5907.554 kb on + strand, within PP_5180 at 5907.555 kb on - strand, within PP_5180 at 5907.582 kb on - strand, within PP_5180 at 5907.582 kb on - strand, within PP_5180 at 5907.654 kb on - strand, within PP_5180
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,904,662 + -0.2 5,904,663 - +0.8 5,904,663 - -0.6 5,904,689 + -0.2 5,904,690 - +0.7 5,904,773 + +0.3 5,904,881 + PP_5178 0.15 -0.9 5,904,993 + PP_5178 0.27 +0.3 5,905,071 - PP_5178 0.36 +0.5 5,905,112 - PP_5178 0.40 +0.6 5,905,112 - PP_5178 0.40 -0.3 5,905,112 - PP_5178 0.40 +1.6 5,905,112 - PP_5178 0.40 +0.5 5,905,117 + PP_5178 0.41 +1.2 5,905,117 + -0.1 5,905,118 - PP_5178 0.41 -0.2 5,905,118 - PP_5178 0.41 -1.0 5,905,133 - PP_5178 0.43 +0.6 5,905,133 - PP_5178 0.43 -0.2 5,905,133 - PP_5178 0.43 +0.8 5,905,240 + PP_5178 0.54 +0.2 5,905,400 - PP_5178 0.72 -0.1 5,905,411 + PP_5178 0.73 -1.2 5,905,412 - PP_5178 0.73 +0.5 5,905,412 - PP_5178 0.73 +1.7 5,905,412 - PP_5178 0.73 -0.2 5,905,438 + PP_5178 0.76 +0.5 5,905,439 - PP_5178 0.76 -0.8 5,905,480 + -0.9 5,905,481 - PP_5178 0.80 +0.1 5,905,481 - PP_5178 0.80 +0.0 5,905,486 + PP_5178 0.81 +0.4 5,905,486 + PP_5178 0.81 +0.2 5,905,486 + PP_5178 0.81 +0.4 5,905,486 + PP_5178 0.81 -0.3 5,905,486 + PP_5178 0.81 -0.6 5,905,486 + PP_5178 0.81 -0.2 5,905,487 - PP_5178 0.81 +1.9 5,905,487 - PP_5178 0.81 -0.5 5,905,487 - PP_5178 0.81 -0.2 5,905,487 - PP_5178 0.81 -0.2 5,905,497 - PP_5178 0.82 -1.9 5,905,498 + PP_5178 0.82 -0.2 5,905,697 + +0.7 5,905,697 + -0.3 5,905,697 + +0.2 5,905,697 + +0.4 5,905,697 + +0.4 5,905,697 + +1.6 5,905,697 + -0.7 5,905,698 - +0.0 5,905,698 - +0.4 5,905,698 - +1.2 5,905,701 + +0.6 5,905,701 + -3.0 5,905,701 + +1.9 5,905,701 + -0.3 5,905,701 + +1.4 5,905,701 + -0.1 5,905,701 + -0.3 5,905,701 + +0.1 5,905,701 + +0.7 5,905,701 + -0.1 5,905,702 - +1.2 5,905,702 - +1.2 5,905,702 - -0.6 5,905,702 - -0.6 5,905,702 - +0.1 5,905,702 - +1.9 5,905,730 - +1.3 5,905,792 - PP_5179 0.12 +0.3 5,905,792 - PP_5179 0.12 +2.7 5,905,905 + PP_5179 0.22 +0.2 5,905,905 + PP_5179 0.22 +0.4 5,905,906 - PP_5179 0.22 +0.7 5,905,906 - PP_5179 0.22 +2.5 5,905,953 + PP_5179 0.26 +1.3 5,905,953 + PP_5179 0.26 -0.2 5,905,953 + PP_5179 0.26 -1.6 5,905,953 + PP_5179 0.26 +1.4 5,905,954 - PP_5179 0.26 -0.5 5,906,068 + PP_5179 0.36 +0.3 5,906,069 - PP_5179 0.36 +0.7 5,906,079 + PP_5179 0.37 +0.1 5,906,079 + PP_5179 0.37 +0.2 5,906,079 + PP_5179 0.37 -0.6 5,906,080 - PP_5179 0.37 -1.1 5,906,080 - PP_5179 0.37 +0.9 5,906,368 - PP_5179 0.62 +0.5 5,906,494 - PP_5179 0.73 +0.4 5,906,494 - PP_5179 0.73 +0.1 5,906,494 - PP_5179 0.73 +0.0 5,906,527 - PP_5179 0.76 -0.6 5,906,778 + +0.4 5,906,845 + +0.1 5,906,892 - +0.2 5,906,978 + +0.1 5,906,998 + PP_5180 0.12 +0.4 5,906,999 - PP_5180 0.12 +0.0 5,907,053 + PP_5180 0.17 -0.5 5,907,054 - PP_5180 0.17 -1.2 5,907,054 - PP_5180 0.17 +1.0 5,907,241 + PP_5180 0.34 +1.7 5,907,241 + PP_5180 0.34 +2.1 5,907,241 + PP_5180 0.34 +1.2 5,907,242 - PP_5180 0.34 -0.2 5,907,242 - PP_5180 0.34 -1.7 5,907,299 + PP_5180 0.39 -0.0 5,907,300 - PP_5180 0.39 -0.4 5,907,300 - PP_5180 0.39 -2.1 5,907,300 - PP_5180 0.39 -0.0 5,907,317 + PP_5180 0.41 +1.9 5,907,317 + PP_5180 0.41 +1.4 5,907,317 + PP_5180 0.41 +0.1 5,907,317 + PP_5180 0.41 +0.5 5,907,326 + PP_5180 0.41 -0.8 5,907,326 + PP_5180 0.41 +0.8 5,907,326 + PP_5180 0.41 +0.8 5,907,326 + PP_5180 0.41 +0.2 5,907,327 - PP_5180 0.42 +0.5 5,907,365 + PP_5180 0.45 +0.8 5,907,365 + PP_5180 0.45 +1.4 5,907,366 - PP_5180 0.45 +0.2 5,907,404 + PP_5180 0.49 -1.0 5,907,404 + PP_5180 0.49 +1.7 5,907,404 + PP_5180 0.49 +0.3 5,907,405 - PP_5180 0.49 +0.4 5,907,405 - PP_5180 0.49 -0.0 5,907,405 - PP_5180 0.49 +0.7 5,907,405 - PP_5180 0.49 -0.2 5,907,405 - PP_5180 0.49 -0.4 5,907,554 + PP_5180 0.62 +0.6 5,907,554 + PP_5180 0.62 +1.0 5,907,555 - PP_5180 0.62 +0.6 5,907,582 - PP_5180 0.65 -0.3 5,907,582 - PP_5180 0.65 +0.7 5,907,654 - PP_5180 0.71 +0.4
Or see this region's nucleotide sequence