Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4550 and PP_4551 are separated by 218 nucleotides PP_4551 and PP_4552 are separated by 34 nucleotides PP_4552 and PP_4553 are separated by 116 nucleotides
PP_4550: PP_4550 - long-chain-fatty-acid/CoA ligase, at 5,173,291 to 5,174,979
_4550
PP_4551: PP_4551 - Hydrolase, alpha/beta fold family, at 5,175,198 to 5,176,142
_4551
PP_4552: PP_4552 - putative conserved enzyme of unknown function, at 5,176,177 to 5,176,647
_4552
PP_4553: PP_4553 - RNA polymerase sigma-70 factor, ECF subfamily, at 5,176,764 to 5,177,984
_4553
Position (kb)
5175
5176
5177 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5174.444 kb on + strand, within PP_4550 at 5174.598 kb on - strand, within PP_4550 at 5174.825 kb on + strand at 5174.825 kb on + strand at 5174.826 kb on - strand at 5174.912 kb on + strand at 5174.913 kb on - strand at 5174.920 kb on + strand at 5174.981 kb on + strand at 5174.982 kb on - strand at 5174.982 kb on - strand at 5174.984 kb on + strand at 5174.984 kb on + strand at 5174.984 kb on + strand at 5174.984 kb on + strand at 5174.985 kb on - strand at 5175.019 kb on + strand at 5175.019 kb on + strand at 5175.020 kb on - strand at 5175.020 kb on - strand at 5175.020 kb on - strand at 5175.027 kb on + strand at 5175.028 kb on - strand at 5175.028 kb on - strand at 5175.068 kb on - strand at 5175.074 kb on + strand at 5175.090 kb on + strand at 5175.090 kb on + strand at 5175.090 kb on + strand at 5175.090 kb on + strand at 5175.090 kb on + strand at 5175.091 kb on - strand at 5175.091 kb on - strand at 5175.091 kb on - strand at 5175.091 kb on - strand at 5175.092 kb on + strand at 5175.092 kb on + strand at 5175.092 kb on + strand at 5175.092 kb on + strand at 5175.093 kb on - strand at 5175.093 kb on - strand at 5175.093 kb on - strand at 5175.093 kb on - strand at 5175.125 kb on + strand at 5175.126 kb on - strand at 5175.126 kb on - strand at 5175.126 kb on - strand at 5175.161 kb on + strand at 5175.161 kb on + strand at 5175.161 kb on + strand at 5175.162 kb on - strand at 5175.162 kb on - strand at 5175.162 kb on - strand at 5175.162 kb on - strand at 5175.246 kb on + strand at 5175.250 kb on + strand at 5175.250 kb on + strand at 5175.250 kb on + strand at 5175.250 kb on + strand at 5175.250 kb on + strand at 5175.250 kb on + strand at 5175.251 kb on - strand at 5175.251 kb on - strand at 5175.251 kb on - strand at 5175.251 kb on - strand at 5175.251 kb on - strand at 5175.277 kb on + strand at 5175.277 kb on + strand at 5175.277 kb on + strand at 5175.547 kb on - strand, within PP_4551 at 5175.547 kb on - strand, within PP_4551 at 5175.547 kb on - strand, within PP_4551 at 5175.561 kb on + strand, within PP_4551 at 5175.561 kb on + strand, within PP_4551 at 5175.616 kb on - strand, within PP_4551 at 5175.616 kb on - strand, within PP_4551 at 5175.616 kb on - strand, within PP_4551 at 5175.798 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.012 kb on + strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.013 kb on - strand, within PP_4551 at 5176.026 kb on + strand, within PP_4551 at 5176.048 kb on + strand at 5176.712 kb on + strand at 5176.794 kb on - strand at 5176.913 kb on - strand, within PP_4553 at 5176.913 kb on - strand, within PP_4553 at 5176.913 kb on - strand, within PP_4553 at 5177.047 kb on + strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.048 kb on - strand, within PP_4553 at 5177.129 kb on + strand, within PP_4553 at 5177.130 kb on - strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.131 kb on + strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553 at 5177.132 kb on - strand, within PP_4553
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,174,444 + PP_4550 0.68 +0.1 5,174,598 - PP_4550 0.77 +0.5 5,174,825 + -2.6 5,174,825 + +1.3 5,174,826 - -1.4 5,174,912 + +0.0 5,174,913 - -0.1 5,174,920 + -0.9 5,174,981 + +0.5 5,174,982 - -1.9 5,174,982 - -0.8 5,174,984 + +0.1 5,174,984 + +0.2 5,174,984 + -0.7 5,174,984 + +0.8 5,174,985 - -0.2 5,175,019 + -0.6 5,175,019 + -0.0 5,175,020 - -1.6 5,175,020 - -0.8 5,175,020 - +0.5 5,175,027 + +0.8 5,175,028 - +0.2 5,175,028 - +0.2 5,175,068 - +1.4 5,175,074 + +1.2 5,175,090 + +1.2 5,175,090 + +1.5 5,175,090 + +0.1 5,175,090 + -0.1 5,175,090 + +0.1 5,175,091 - +0.5 5,175,091 - -0.6 5,175,091 - +0.2 5,175,091 - +1.9 5,175,092 + +1.3 5,175,092 + +0.5 5,175,092 + -0.3 5,175,092 + -1.4 5,175,093 - -0.7 5,175,093 - -0.3 5,175,093 - +1.7 5,175,093 - -0.6 5,175,125 + -2.2 5,175,126 - -0.3 5,175,126 - -0.5 5,175,126 - -0.1 5,175,161 + -0.8 5,175,161 + +1.1 5,175,161 + +0.3 5,175,162 - -1.4 5,175,162 - +1.7 5,175,162 - -1.1 5,175,162 - -0.2 5,175,246 + -1.8 5,175,250 + -0.9 5,175,250 + -0.2 5,175,250 + -1.0 5,175,250 + -0.1 5,175,250 + -0.6 5,175,250 + -0.1 5,175,251 - +0.2 5,175,251 - -0.1 5,175,251 - -0.1 5,175,251 - +0.1 5,175,251 - -0.5 5,175,277 + -0.4 5,175,277 + -0.2 5,175,277 + +2.8 5,175,547 - PP_4551 0.37 -0.5 5,175,547 - PP_4551 0.37 +1.5 5,175,547 - PP_4551 0.37 +0.1 5,175,561 + PP_4551 0.38 +0.7 5,175,561 + PP_4551 0.38 +0.8 5,175,616 - PP_4551 0.44 -0.2 5,175,616 - PP_4551 0.44 +1.5 5,175,616 - PP_4551 0.44 +0.3 5,175,798 + PP_4551 0.63 +0.2 5,176,012 + PP_4551 0.86 -0.3 5,176,012 + PP_4551 0.86 +2.6 5,176,012 + PP_4551 0.86 +0.5 5,176,012 + PP_4551 0.86 -0.4 5,176,012 + PP_4551 0.86 +0.7 5,176,013 - PP_4551 0.86 +1.2 5,176,013 - PP_4551 0.86 +0.5 5,176,013 - PP_4551 0.86 -1.1 5,176,013 - PP_4551 0.86 +1.0 5,176,013 - PP_4551 0.86 +1.9 5,176,013 - PP_4551 0.86 -0.4 5,176,013 - PP_4551 0.86 +2.0 5,176,013 - PP_4551 0.86 +0.6 5,176,013 - PP_4551 0.86 +1.2 5,176,026 + PP_4551 0.88 -3.7 5,176,048 + -0.0 5,176,712 + +0.1 5,176,794 - -1.1 5,176,913 - PP_4553 0.12 -0.3 5,176,913 - PP_4553 0.12 -0.9 5,176,913 - PP_4553 0.12 -0.1 5,177,047 + PP_4553 0.23 -0.3 5,177,048 - PP_4553 0.23 +1.0 5,177,048 - PP_4553 0.23 +0.1 5,177,048 - PP_4553 0.23 -0.4 5,177,129 + PP_4553 0.30 +0.0 5,177,130 - PP_4553 0.30 -1.1 5,177,131 + PP_4553 0.30 -0.2 5,177,131 + PP_4553 0.30 -0.3 5,177,131 + PP_4553 0.30 +0.4 5,177,132 - PP_4553 0.30 -0.4 5,177,132 - PP_4553 0.30 -0.8
Or see this region's nucleotide sequence