Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4519 and PP_4520 are separated by 62 nucleotides PP_4520 and PP_4521 are separated by 64 nucleotides
PP_4519: PP_4519 - Agglutination protein, at 5,132,919 to 5,134,277
_4519
PP_4520: PP_4520 - conserved protein of unknown function, at 5,134,340 to 5,134,939
_4520
PP_4521: PP_4521 - putative aerotaxis receptor, at 5,135,004 to 5,136,569
_4521
Position (kb) 5134
5135 Strain fitness (log2 ratio) -1
0
1
2 at 5133.666 kb on + strand, within PP_4519 at 5133.666 kb on + strand, within PP_4519 at 5133.666 kb on + strand, within PP_4519 at 5133.666 kb on + strand, within PP_4519 at 5133.667 kb on - strand, within PP_4519 at 5133.667 kb on - strand, within PP_4519 at 5133.711 kb on + strand, within PP_4519 at 5133.711 kb on + strand, within PP_4519 at 5133.712 kb on - strand, within PP_4519 at 5133.735 kb on + strand, within PP_4519 at 5133.735 kb on + strand, within PP_4519 at 5133.736 kb on - strand, within PP_4519 at 5133.736 kb on - strand, within PP_4519 at 5133.736 kb on - strand, within PP_4519 at 5133.837 kb on + strand, within PP_4519 at 5133.837 kb on + strand, within PP_4519 at 5133.837 kb on + strand, within PP_4519 at 5133.838 kb on - strand, within PP_4519 at 5133.838 kb on - strand, within PP_4519 at 5133.874 kb on + strand, within PP_4519 at 5133.875 kb on - strand, within PP_4519 at 5133.875 kb on - strand, within PP_4519 at 5133.875 kb on - strand, within PP_4519 at 5133.875 kb on - strand, within PP_4519 at 5134.009 kb on - strand, within PP_4519 at 5134.108 kb on - strand, within PP_4519 at 5134.205 kb on + strand at 5134.206 kb on - strand at 5134.206 kb on - strand at 5134.357 kb on + strand at 5134.357 kb on + strand at 5134.358 kb on - strand at 5134.358 kb on - strand at 5134.358 kb on - strand at 5134.373 kb on + strand at 5134.373 kb on + strand at 5134.374 kb on - strand at 5134.587 kb on + strand, within PP_4520 at 5134.588 kb on - strand, within PP_4520 at 5134.588 kb on - strand, within PP_4520 at 5134.588 kb on - strand, within PP_4520 at 5134.663 kb on - strand, within PP_4520 at 5134.663 kb on - strand, within PP_4520 at 5134.663 kb on - strand, within PP_4520 at 5134.663 kb on - strand, within PP_4520 at 5134.889 kb on + strand at 5134.890 kb on - strand at 5134.890 kb on - strand at 5135.031 kb on - strand at 5135.031 kb on - strand at 5135.038 kb on + strand at 5135.342 kb on + strand, within PP_4521 at 5135.343 kb on - strand, within PP_4521 at 5135.343 kb on - strand, within PP_4521 at 5135.514 kb on - strand, within PP_4521 at 5135.514 kb on - strand, within PP_4521 at 5135.514 kb on - strand, within PP_4521 at 5135.667 kb on - strand, within PP_4521 at 5135.667 kb on - strand, within PP_4521 at 5135.917 kb on + strand, within PP_4521 at 5135.918 kb on - strand, within PP_4521 at 5135.918 kb on - strand, within PP_4521
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 5,133,666 + PP_4519 0.55 +0.4 5,133,666 + PP_4519 0.55 +0.0 5,133,666 + PP_4519 0.55 +0.2 5,133,666 + PP_4519 0.55 +1.4 5,133,667 - PP_4519 0.55 +0.7 5,133,667 - PP_4519 0.55 +1.1 5,133,711 + PP_4519 0.58 +0.1 5,133,711 + PP_4519 0.58 +0.1 5,133,712 - PP_4519 0.58 +0.6 5,133,735 + PP_4519 0.60 +0.4 5,133,735 + PP_4519 0.60 +0.0 5,133,736 - PP_4519 0.60 +0.6 5,133,736 - PP_4519 0.60 +0.2 5,133,736 - PP_4519 0.60 +0.3 5,133,837 + PP_4519 0.68 -1.2 5,133,837 + PP_4519 0.68 -0.4 5,133,837 + PP_4519 0.68 +2.1 5,133,838 - PP_4519 0.68 -0.0 5,133,838 - PP_4519 0.68 -0.8 5,133,874 + PP_4519 0.70 +0.8 5,133,875 - PP_4519 0.70 -0.0 5,133,875 - PP_4519 0.70 -0.7 5,133,875 - PP_4519 0.70 -0.7 5,133,875 - PP_4519 0.70 -0.2 5,134,009 - PP_4519 0.80 -1.4 5,134,108 - PP_4519 0.87 -0.7 5,134,205 + +0.4 5,134,206 - -0.7 5,134,206 - +0.3 5,134,357 + -0.1 5,134,357 + +0.7 5,134,358 - -0.3 5,134,358 - -1.1 5,134,358 - +0.7 5,134,373 + -0.5 5,134,373 + +0.2 5,134,374 - -1.3 5,134,587 + PP_4520 0.41 +0.9 5,134,588 - PP_4520 0.41 -0.3 5,134,588 - PP_4520 0.41 +0.9 5,134,588 - PP_4520 0.41 +0.3 5,134,663 - PP_4520 0.54 +2.1 5,134,663 - PP_4520 0.54 +0.0 5,134,663 - PP_4520 0.54 -0.0 5,134,663 - PP_4520 0.54 +0.9 5,134,889 + -0.5 5,134,890 - +0.4 5,134,890 - -0.6 5,135,031 - -1.7 5,135,031 - +0.1 5,135,038 + -0.4 5,135,342 + PP_4521 0.22 +0.6 5,135,343 - PP_4521 0.22 -1.1 5,135,343 - PP_4521 0.22 +0.7 5,135,514 - PP_4521 0.33 +0.3 5,135,514 - PP_4521 0.33 -1.0 5,135,514 - PP_4521 0.33 +0.1 5,135,667 - PP_4521 0.42 -1.4 5,135,667 - PP_4521 0.42 +0.3 5,135,917 + PP_4521 0.58 -0.3 5,135,918 - PP_4521 0.58 -0.4 5,135,918 - PP_4521 0.58 -0.7
Or see this region's nucleotide sequence