Strain Fitness in Pseudomonas putida KT2440 around PP_4122

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
4,658,157	+				+0.3
4,658,158	-		PP_4121	0.45	+0.8
4,658,176	-		PP_4121	0.46	-0.5
4,658,205	+				+1.7
4,658,318	+		PP_4121	0.53	-1.4
4,658,323	-		PP_4121	0.54	+1.2
4,658,323	-		PP_4121	0.54	+0.7
4,658,323	-		PP_4121	0.54	+3.7
4,658,323	-		PP_4121	0.54	+0.7
4,658,323	-		PP_4121	0.54	-0.7
4,658,527	-		PP_4121	0.65	+0.7
4,658,568	-		PP_4121	0.68	-1.3
4,658,586	+				-1.5
4,658,663	+		PP_4121	0.73	+0.1
4,658,664	-		PP_4121	0.73	-1.2
4,658,677	-		PP_4121	0.74	+2.5
4,658,708	-		PP_4121	0.75	-1.0
4,658,774	+		PP_4121	0.79	+0.3
4,658,979	+				-0.4
4,658,979	+				-0.1
4,658,980	-				-1.3
4,658,983	-				-0.1
4,659,102	+				-0.4
4,659,103	-				+0.3
4,659,113	+				-1.2
4,659,164	-				-0.6
4,659,230	+		PP_4122	0.16	-0.6
4,659,231	-		PP_4122	0.16	-0.7
4,659,231	-		PP_4122	0.16	+1.9
4,659,437	+		PP_4122	0.58	+1.9
4,659,438	-		PP_4122	0.58	+1.5
4,659,638	+				+1.7
4,659,638	+				-1.8
4,659,639	-				-1.0
4,659,639	-				-1.8
4,659,639	-				-0.3
4,659,642	+				+2.9
4,659,642	+				-0.7
4,659,642	+				+2.7
4,659,642	+				+1.4
4,659,642	+				-2.0
4,659,643	-				-0.3
4,659,643	-				-1.0
4,659,643	-				+0.0
4,659,651	+				+2.4
4,659,651	+				-1.2
4,659,651	+				-1.4
4,659,651	+				+0.3
4,659,652	-				-0.1
4,659,652	-				-0.8
4,659,652	-				-1.2
4,659,652	-				-0.3
4,660,373	-		PP_4123	0.54	-0.7
4,660,501	+		PP_4123	0.63	-0.2