Strain Fitness in Pseudomonas putida KT2440 around PP_3729

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
4,255,154	+		PP_3728	0.57	+0.5
4,255,155	-		PP_3728	0.57	+0.7
4,255,155	-		PP_3728	0.57	-1.5
4,255,199	+		PP_3728	0.59	+0.3
4,255,200	-				+0.8
4,255,272	+		PP_3728	0.62	-2.0
4,255,272	+		PP_3728	0.62	-0.8
4,255,421	-		PP_3728	0.69	+0.0
4,255,476	+		PP_3728	0.71	+0.5
4,255,623	-		PP_3728	0.78	+0.9
4,255,623	-		PP_3728	0.78	+1.6
4,255,623	-		PP_3728	0.78	+0.9
4,255,700	+		PP_3728	0.81	-0.2
4,255,700	+		PP_3728	0.81	-0.1
4,255,700	+		PP_3728	0.81	+0.3
4,255,700	+		PP_3728	0.81	+0.2
4,255,782	-		PP_3728	0.85	-0.3
4,255,782	-		PP_3728	0.85	-1.1
4,255,782	-		PP_3728	0.85	+1.0
4,255,782	-		PP_3728	0.85	+0.0
4,256,028	-				-0.1
4,256,161	+				-0.6
4,256,383	+		PP_3729	0.33	+0.4
4,256,383	+		PP_3729	0.33	-0.4
4,256,384	-		PP_3729	0.33	+0.4
4,256,384	-		PP_3729	0.33	+0.9
4,256,485	+		PP_3729	0.46	+0.3
4,256,486	-		PP_3729	0.46	+2.0
4,256,486	-		PP_3729	0.46	-0.9
4,256,497	+		PP_3729	0.48	+2.3
4,256,567	-		PP_3729	0.57	-0.8
4,256,615	+		PP_3729	0.63	+0.3
4,256,642	-		PP_3729	0.66	+1.3
4,256,932	-				+0.2
4,256,932	-				+0.4
4,257,325	-				-1.8
4,257,366	+		PP_3730	0.65	-0.4
4,257,533	+				+0.7
4,257,534	-		PP_3730	0.88	-0.4
4,257,558	+				+0.7
4,257,559	-				-0.2
4,257,559	-				-0.6
4,257,651	+				+1.0
4,257,652	-				-0.1
4,257,652	-				-0.4
4,257,652	-				+0.2
4,257,652	-				-0.2
4,257,672	+				-0.2
4,257,673	-				+0.2
4,257,673	-				-1.4
4,257,906	+		PP_3731	0.33	+0.7