Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3646 and PP_3647 are separated by 12 nucleotides PP_3647 and PP_3648 are separated by 122 nucleotides PP_3648 and PP_3649 are separated by 88 nucleotides
PP_3646: PP_3646 - Aldehyde dehydrogenase family protein, at 4,141,868 to 4,143,349
_3646
PP_3647: PP_3647 - putative Oxidoreductase, at 4,143,362 to 4,143,847
_3647
PP_3648: PP_3648 - Carboxymuconolactone decarboxylase family protein, at 4,143,970 to 4,144,359
_3648
PP_3649: PP_3649 - Transcriptional regulator, GntR family, at 4,144,448 to 4,145,200
_3649
Position (kb)
4143
4144 Strain fitness (log2 ratio)
-1
0
1
2 at 4142.492 kb on + strand, within PP_3646 at 4142.583 kb on + strand, within PP_3646 at 4142.702 kb on + strand, within PP_3646 at 4142.702 kb on + strand, within PP_3646 at 4142.702 kb on + strand, within PP_3646 at 4142.703 kb on - strand, within PP_3646 at 4142.703 kb on - strand, within PP_3646 at 4142.966 kb on + strand, within PP_3646 at 4142.967 kb on - strand, within PP_3646 at 4142.967 kb on - strand, within PP_3646 at 4143.192 kb on - strand, within PP_3646 at 4143.219 kb on + strand at 4143.219 kb on + strand at 4143.220 kb on - strand at 4143.279 kb on - strand at 4143.279 kb on - strand at 4143.347 kb on + strand at 4143.347 kb on + strand at 4143.348 kb on - strand at 4143.348 kb on - strand at 4143.380 kb on + strand at 4143.521 kb on + strand, within PP_3647 at 4143.537 kb on - strand, within PP_3647 at 4143.605 kb on + strand, within PP_3647 at 4143.606 kb on - strand, within PP_3647 at 4143.725 kb on + strand, within PP_3647 at 4143.806 kb on + strand at 4143.806 kb on + strand at 4143.806 kb on + strand at 4143.807 kb on - strand at 4143.848 kb on + strand at 4143.848 kb on + strand at 4143.848 kb on + strand at 4143.849 kb on - strand at 4143.849 kb on - strand at 4143.849 kb on - strand at 4143.849 kb on - strand at 4143.963 kb on - strand at 4143.963 kb on - strand at 4144.030 kb on + strand, within PP_3648 at 4144.031 kb on - strand, within PP_3648 at 4144.031 kb on - strand, within PP_3648 at 4144.096 kb on + strand, within PP_3648 at 4144.096 kb on + strand, within PP_3648 at 4144.097 kb on - strand, within PP_3648 at 4144.155 kb on - strand, within PP_3648 at 4144.155 kb on - strand, within PP_3648 at 4144.276 kb on + strand, within PP_3648 at 4144.276 kb on + strand, within PP_3648 at 4144.276 kb on + strand, within PP_3648 at 4144.434 kb on - strand at 4144.434 kb on - strand at 4144.499 kb on - strand at 4144.620 kb on + strand, within PP_3649 at 4144.724 kb on + strand, within PP_3649 at 4144.724 kb on + strand, within PP_3649 at 4144.724 kb on + strand, within PP_3649 at 4144.724 kb on + strand, within PP_3649 at 4144.725 kb on - strand, within PP_3649 at 4144.725 kb on - strand, within PP_3649 at 4144.725 kb on - strand, within PP_3649 at 4144.725 kb on - strand, within PP_3649
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 4,142,492 + PP_3646 0.42 +0.9 4,142,583 + PP_3646 0.48 -0.1 4,142,702 + PP_3646 0.56 +0.9 4,142,702 + PP_3646 0.56 +0.7 4,142,702 + PP_3646 0.56 +0.0 4,142,703 - PP_3646 0.56 -1.0 4,142,703 - PP_3646 0.56 +0.0 4,142,966 + PP_3646 0.74 -0.8 4,142,967 - PP_3646 0.74 +0.4 4,142,967 - PP_3646 0.74 -1.2 4,143,192 - PP_3646 0.89 +0.8 4,143,219 + -0.6 4,143,219 + +1.4 4,143,220 - -0.4 4,143,279 - -0.3 4,143,279 - -0.3 4,143,347 + -0.3 4,143,347 + -1.3 4,143,348 - +0.2 4,143,348 - -0.1 4,143,380 + -0.1 4,143,521 + PP_3647 0.33 +1.8 4,143,537 - PP_3647 0.36 +0.4 4,143,605 + PP_3647 0.50 +1.1 4,143,606 - PP_3647 0.50 -0.4 4,143,725 + PP_3647 0.75 +0.2 4,143,806 + +0.1 4,143,806 + +0.6 4,143,806 + -1.2 4,143,807 - -0.2 4,143,848 + -0.5 4,143,848 + -1.4 4,143,848 + +1.2 4,143,849 - -0.5 4,143,849 - -0.3 4,143,849 - +0.7 4,143,849 - +0.4 4,143,963 - +2.2 4,143,963 - -0.7 4,144,030 + PP_3648 0.15 -0.4 4,144,031 - PP_3648 0.16 -1.1 4,144,031 - PP_3648 0.16 -0.5 4,144,096 + PP_3648 0.32 +0.1 4,144,096 + PP_3648 0.32 -0.5 4,144,097 - PP_3648 0.33 -1.0 4,144,155 - PP_3648 0.47 +0.2 4,144,155 - PP_3648 0.47 -0.8 4,144,276 + PP_3648 0.78 +0.8 4,144,276 + PP_3648 0.78 +0.1 4,144,276 + PP_3648 0.78 -1.4 4,144,434 - -1.1 4,144,434 - -0.8 4,144,499 - -0.6 4,144,620 + PP_3649 0.23 -0.1 4,144,724 + PP_3649 0.37 +0.3 4,144,724 + PP_3649 0.37 -0.6 4,144,724 + PP_3649 0.37 -1.4 4,144,724 + PP_3649 0.37 -1.1 4,144,725 - PP_3649 0.37 +0.5 4,144,725 - PP_3649 0.37 +0.4 4,144,725 - PP_3649 0.37 +0.4 4,144,725 - PP_3649 0.37 -0.1
Or see this region's nucleotide sequence