Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3417 and PP_3418 are separated by 86 nucleotides PP_3418 and PP_3419 are separated by 14 nucleotides PP_3419 and PP_3420 overlap by 32 nucleotides
PP_3417: PP_3417 - D-gluconate transporter, at 3,867,735 to 3,869,087
_3417
PP_3418: PP_3418 - conserved protein of unknown function, at 3,869,174 to 3,869,476
_3418
PP_3419: PP_3419 - Sigma-54 dependent transcriptional regulator/response regulator, at 3,869,491 to 3,870,918
_3419
PP_3420: PP_3420 - Sensor histidine kinase, at 3,870,887 to 3,872,380
_3420
Position (kb)
3869
3870
3871 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3868.856 kb on + strand, within PP_3417 at 3868.909 kb on + strand, within PP_3417 at 3868.981 kb on - strand at 3868.981 kb on - strand at 3868.981 kb on - strand at 3869.101 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.140 kb on + strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.141 kb on - strand at 3869.156 kb on - strand at 3869.156 kb on - strand at 3869.347 kb on - strand, within PP_3418 at 3869.467 kb on + strand at 3869.490 kb on - strand at 3869.490 kb on - strand at 3869.492 kb on + strand at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.832 kb on + strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3869.833 kb on - strand, within PP_3419 at 3870.184 kb on - strand, within PP_3419 at 3870.601 kb on + strand, within PP_3419 at 3870.839 kb on + strand at 3870.840 kb on - strand at 3870.857 kb on + strand at 3870.858 kb on - strand at 3870.938 kb on - strand at 3870.946 kb on + strand at 3870.946 kb on + strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3870.947 kb on - strand at 3871.508 kb on + strand, within PP_3420 at 3871.508 kb on + strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.509 kb on - strand, within PP_3420 at 3871.572 kb on + strand, within PP_3420 at 3871.573 kb on - strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.615 kb on + strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.616 kb on - strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.653 kb on + strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.654 kb on - strand, within PP_3420 at 3871.698 kb on + strand, within PP_3420 at 3871.699 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.761 kb on - strand, within PP_3420 at 3871.794 kb on + strand, within PP_3420 at 3871.812 kb on + strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.813 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420 at 3871.867 kb on - strand, within PP_3420
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,868,856 + PP_3417 0.83 +0.2 3,868,909 + PP_3417 0.87 -0.3 3,868,981 - -0.7 3,868,981 - -0.6 3,868,981 - -0.3 3,869,101 + -0.1 3,869,140 + -0.1 3,869,140 + +0.3 3,869,140 + +0.4 3,869,141 - +1.0 3,869,141 - +0.1 3,869,141 - +0.9 3,869,141 - -0.3 3,869,156 - -0.7 3,869,156 - +1.3 3,869,347 - PP_3418 0.57 -1.5 3,869,467 + +1.1 3,869,490 - -0.4 3,869,490 - +0.8 3,869,492 + +0.6 3,869,832 + PP_3419 0.24 +0.3 3,869,832 + PP_3419 0.24 +1.1 3,869,832 + PP_3419 0.24 -0.0 3,869,832 + PP_3419 0.24 +0.8 3,869,833 - PP_3419 0.24 +0.8 3,869,833 - PP_3419 0.24 +0.1 3,870,184 - PP_3419 0.49 +0.8 3,870,601 + PP_3419 0.78 -0.2 3,870,839 + +0.5 3,870,840 - +0.1 3,870,857 + +0.3 3,870,858 - +0.0 3,870,938 - -1.2 3,870,946 + +0.2 3,870,946 + +0.3 3,870,947 - -0.5 3,870,947 - +0.6 3,870,947 - +0.4 3,870,947 - +1.3 3,871,508 + PP_3420 0.42 +0.9 3,871,508 + PP_3420 0.42 +0.5 3,871,509 - PP_3420 0.42 -0.2 3,871,509 - PP_3420 0.42 +1.7 3,871,509 - PP_3420 0.42 +0.4 3,871,572 + PP_3420 0.46 +0.3 3,871,573 - PP_3420 0.46 +1.2 3,871,615 + PP_3420 0.49 -0.8 3,871,615 + PP_3420 0.49 +3.0 3,871,615 + PP_3420 0.49 -0.5 3,871,615 + PP_3420 0.49 -1.2 3,871,615 + PP_3420 0.49 -0.7 3,871,615 + PP_3420 0.49 +1.8 3,871,615 + PP_3420 0.49 +0.2 3,871,615 + PP_3420 0.49 +0.3 3,871,615 + PP_3420 0.49 -0.8 3,871,616 - PP_3420 0.49 +1.1 3,871,616 - PP_3420 0.49 -0.4 3,871,616 - PP_3420 0.49 -0.2 3,871,616 - PP_3420 0.49 -0.7 3,871,616 - PP_3420 0.49 +0.0 3,871,653 + PP_3420 0.51 +0.8 3,871,653 + PP_3420 0.51 -0.6 3,871,654 - PP_3420 0.51 -1.0 3,871,654 - PP_3420 0.51 +2.5 3,871,698 + PP_3420 0.54 -0.8 3,871,699 - PP_3420 0.54 +0.8 3,871,761 - PP_3420 0.59 +0.4 3,871,761 - PP_3420 0.59 +0.3 3,871,794 + PP_3420 0.61 -0.3 3,871,812 + PP_3420 0.62 -1.0 3,871,813 - PP_3420 0.62 +0.2 3,871,813 - PP_3420 0.62 -0.3 3,871,867 - PP_3420 0.66 -0.0 3,871,867 - PP_3420 0.66 +0.2
Or see this region's nucleotide sequence