Strain Fitness in Pseudomonas putida KT2440 around PP_3336

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_5743 and PP_3335 are separated by 65 nucleotidesPP_3335 and PP_3336 are separated by 50 nucleotidesPP_3336 and PP_3337 are separated by 103 nucleotidesPP_3337 and PP_3338 overlap by 20 nucleotidesPP_3338 and PP_3339 are separated by 142 nucleotides PP_5743: PP_5743 - putative TonB-dependent receptor protein, at 3,773,298 to 3,774,305 _5743 PP_3335: PP_3335 - conserved protein of unknown function, at 3,774,371 to 3,774,973 _3335 PP_3336: PP_3336 - conserved protein of unknown function, at 3,775,024 to 3,775,476 _3336 PP_3337: PP_3337 - conserved membrane protein of unknown function, at 3,775,580 to 3,775,975 _3337 PP_3338: PP_3338 - putative cytochrome bd-type quinol oxidase, at 3,775,956 to 3,776,318 _3338 PP_3339: PP_3339 - conserved exported protein of unknown function, at 3,776,461 to 3,776,868 _3339 Position (kb) 3775 3776Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3774.070 kb on - strand, within PP_5743at 3774.104 kb on - strand, within PP_5743at 3774.288 kb on + strandat 3774.289 kb on - strandat 3774.289 kb on - strandat 3774.619 kb on + strand, within PP_3335at 3774.620 kb on - strand, within PP_3335at 3774.620 kb on - strand, within PP_3335at 3774.620 kb on - strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.681 kb on + strand, within PP_3335at 3774.682 kb on - strand, within PP_3335at 3774.682 kb on - strand, within PP_3335at 3774.684 kb on + strand, within PP_3335at 3774.715 kb on - strand, within PP_3335at 3774.794 kb on + strand, within PP_3335at 3774.795 kb on - strand, within PP_3335at 3775.218 kb on + strand, within PP_3336at 3775.219 kb on - strand, within PP_3336at 3775.252 kb on + strand, within PP_3336at 3775.271 kb on + strand, within PP_3336at 3775.271 kb on + strand, within PP_3336at 3775.272 kb on - strand, within PP_3336at 3775.315 kb on - strand, within PP_3336at 3775.337 kb on - strand, within PP_3336at 3775.504 kb on + strandat 3775.505 kb on - strandat 3775.535 kb on + strandat 3775.535 kb on + strandat 3775.535 kb on + strandat 3775.566 kb on - strandat 3775.621 kb on - strand, within PP_3337at 3775.621 kb on - strand, within PP_3337at 3775.707 kb on - strand, within PP_3337at 3775.889 kb on + strand, within PP_3337at 3775.890 kb on - strand, within PP_3337at 3775.890 kb on - strand, within PP_3337at 3775.890 kb on - strand, within PP_3337at 3775.911 kb on + strand, within PP_3337at 3775.911 kb on + strand, within PP_3337at 3775.911 kb on + strand, within PP_3337at 3775.912 kb on - strand, within PP_3337at 3775.912 kb on - strand, within PP_3337at 3775.971 kb on - strandat 3776.113 kb on + strand, within PP_3338at 3776.113 kb on + strand, within PP_3338at 3776.114 kb on - strand, within PP_3338at 3776.114 kb on - strand, within PP_3338at 3776.114 kb on - strand, within PP_3338at 3776.182 kb on - strand, within PP_3338at 3776.281 kb on + strand, within PP_3338at 3776.408 kb on + strandat 3776.431 kb on + strandat 3776.432 kb on - strandat 3776.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml
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3,774,070 - PP_5743 0.77 -0.6
3,774,104 - PP_5743 0.80 -0.4
3,774,288 + -0.1
3,774,289 - +1.1
3,774,289 - +1.0
3,774,619 + PP_3335 0.41 -0.5
3,774,620 - PP_3335 0.41 +1.4
3,774,620 - PP_3335 0.41 +2.0
3,774,620 - PP_3335 0.41 -0.5
3,774,681 + PP_3335 0.51 -0.2
3,774,681 + PP_3335 0.51 -0.8
3,774,681 + PP_3335 0.51 +0.1
3,774,681 + PP_3335 0.51 -0.0
3,774,681 + PP_3335 0.51 +2.5
3,774,681 + PP_3335 0.51 -0.4
3,774,681 + PP_3335 0.51 -0.4
3,774,682 - PP_3335 0.52 +1.6
3,774,682 - PP_3335 0.52 +0.4
3,774,684 + PP_3335 0.52 +0.1
3,774,715 - PP_3335 0.57 +0.5
3,774,794 + PP_3335 0.70 +0.5
3,774,795 - PP_3335 0.70 -0.7
3,775,218 + PP_3336 0.43 +0.7
3,775,219 - PP_3336 0.43 +1.6
3,775,252 + PP_3336 0.50 +1.3
3,775,271 + PP_3336 0.55 +0.2
3,775,271 + PP_3336 0.55 -0.6
3,775,272 - PP_3336 0.55 +1.6
3,775,315 - PP_3336 0.64 -1.0
3,775,337 - PP_3336 0.69 +1.3
3,775,504 + -1.1
3,775,505 - +1.2
3,775,535 + -0.1
3,775,535 + -0.3
3,775,535 + -0.6
3,775,566 - +0.8
3,775,621 - PP_3337 0.10 +0.3
3,775,621 - PP_3337 0.10 -1.7
3,775,707 - PP_3337 0.32 +1.9
3,775,889 + PP_3337 0.78 +0.6
3,775,890 - PP_3337 0.78 -0.7
3,775,890 - PP_3337 0.78 +1.1
3,775,890 - PP_3337 0.78 -0.4
3,775,911 + PP_3337 0.84 -0.3
3,775,911 + PP_3337 0.84 +0.3
3,775,911 + PP_3337 0.84 +0.1
3,775,912 - PP_3337 0.84 +0.2
3,775,912 - PP_3337 0.84 -0.2
3,775,971 - -0.3
3,776,113 + PP_3338 0.43 -1.1
3,776,113 + PP_3338 0.43 +0.6
3,776,114 - PP_3338 0.44 +0.3
3,776,114 - PP_3338 0.44 -1.1
3,776,114 - PP_3338 0.44 -0.9
3,776,182 - PP_3338 0.62 -0.5
3,776,281 + PP_3338 0.90 +0.0
3,776,408 + -3.2
3,776,431 + +1.3
3,776,432 - -1.0
3,776,443 + +0.3

Or see this region's nucleotide sequence