Strain Fitness in Pseudomonas putida KT2440 around PP_3231

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
3,666,407	-				-1.8
3,666,506	+		PP_3230	0.20	-0.1
3,666,507	-		PP_3230	0.20	-0.7
3,666,507	-		PP_3230	0.20	-0.1
3,666,556	+		PP_3230	0.28	+0.5
3,666,556	+		PP_3230	0.28	-0.4
3,666,556	+		PP_3230	0.28	-1.0
3,666,640	-		PP_3230	0.40	-1.0
3,666,654	+		PP_3230	0.42	+0.8
3,666,896	+		PP_3230	0.77	-0.2
3,666,896	+		PP_3230	0.77	-0.5
3,666,896	+		PP_3230	0.77	-0.7
3,666,957	-		PP_3230	0.85	+0.3
3,667,139	+				+1.2
3,667,140	-				-0.9
3,667,140	-				+0.2
3,667,143	+				-1.2
3,667,143	+				-0.4
3,667,143	+				+3.0
3,667,143	+				+0.5
3,667,143	+				+4.2
3,667,143	+				-1.9
3,667,144	-				+0.7
3,667,170	+				-0.4
3,667,179	+				+0.7
3,667,180	-				-1.4
3,667,279	-				+1.1
3,667,452	+				-0.4
3,667,453	-				-0.6
3,667,483	-		PP_3231	0.12	+0.5
3,667,483	-		PP_3231	0.12	-1.0
3,667,509	-		PP_3231	0.14	+0.7
3,667,927	-		PP_3231	0.44	+1.2
3,667,929	+		PP_3231	0.45	+2.7
3,667,949	+		PP_3231	0.46	+0.9
3,667,949	+		PP_3231	0.46	-0.7
3,668,091	+		PP_3231	0.56	+1.0
3,668,178	+		PP_3231	0.63	+0.1
3,668,215	-		PP_3231	0.65	+0.5
3,668,215	-		PP_3231	0.65	-4.1
3,668,595	+				-0.6
3,668,615	+				-0.9
3,668,616	-				+0.1
3,668,616	-				+0.2
3,668,638	-				-0.3
3,668,700	+				-0.0
3,668,865	-		PP_3232	0.21	+0.4
3,668,946	+		PP_3232	0.35	+0.2
3,668,946	+		PP_3232	0.35	+0.1
3,668,946	+		PP_3232	0.35	-0.6
3,668,947	-		PP_3232	0.35	+0.9
3,669,053	+		PP_3232	0.54	+0.4
3,669,285	+				-1.2
3,669,285	+				+0.3
3,669,285	+				-0.7
3,669,285	+				-0.1
3,669,286	-				-0.7
3,669,286	-				+0.8
3,669,286	-				+2.1
3,669,289	+				-0.0
3,669,438	-				-0.1
3,669,438	-				+0.3
3,669,471	+				-1.5
3,669,471	+				+0.6
3,669,472	-				-2.6
3,669,472	-				+0.2
3,669,674	-				+0.9