Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3203 and PP_3204 are separated by 157 nucleotides PP_3204 and PP_3205 overlap by 4 nucleotides PP_3205 and PP_3206 overlap by 10 nucleotides PP_3206 and PP_3207 overlap by 4 nucleotides
PP_3203: PP_3203 - Major facilitator family transporter, at 3,636,073 to 3,637,263
_3203
PP_3204: PP_3204 - conserved protein of unknown function, at 3,637,421 to 3,638,008
_3204
PP_3205: PP_3205 - Fumarylacetoacetate hydrolase family protein, at 3,638,005 to 3,638,997
_3205
PP_3206: PP_3206 - conserved protein of unknown function, at 3,638,988 to 3,639,974
_3206
PP_3207: PP_3207 - putative Cyclase, at 3,639,971 to 3,640,753
_3207
Position (kb)
3638
3639 Strain fitness (log2 ratio)
-1
0
1
2 at 3637.299 kb on - strand at 3637.386 kb on - strand at 3637.464 kb on + strand at 3637.464 kb on + strand at 3637.465 kb on - strand at 3637.563 kb on - strand, within PP_3204 at 3637.563 kb on - strand, within PP_3204 at 3637.817 kb on + strand, within PP_3204 at 3637.818 kb on - strand, within PP_3204 at 3638.161 kb on + strand, within PP_3205 at 3638.345 kb on - strand, within PP_3205 at 3638.345 kb on - strand, within PP_3205 at 3638.387 kb on + strand, within PP_3205 at 3638.388 kb on - strand, within PP_3205 at 3638.781 kb on - strand, within PP_3205 at 3639.188 kb on - strand, within PP_3206 at 3639.252 kb on + strand, within PP_3206 at 3639.264 kb on + strand, within PP_3206 at 3639.340 kb on - strand, within PP_3206 at 3639.360 kb on + strand, within PP_3206 at 3639.420 kb on + strand, within PP_3206 at 3639.421 kb on - strand, within PP_3206 at 3639.577 kb on - strand, within PP_3206 at 3639.577 kb on - strand, within PP_3206 at 3639.577 kb on - strand, within PP_3206 at 3639.848 kb on + strand, within PP_3206 at 3639.849 kb on - strand, within PP_3206 at 3639.908 kb on + strand at 3639.970 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,637,299 - -0.1 3,637,386 - -0.1 3,637,464 + +0.3 3,637,464 + -0.1 3,637,465 - -0.3 3,637,563 - PP_3204 0.24 +0.8 3,637,563 - PP_3204 0.24 +0.5 3,637,817 + PP_3204 0.67 +0.2 3,637,818 - PP_3204 0.68 -0.4 3,638,161 + PP_3205 0.16 +0.6 3,638,345 - PP_3205 0.34 +0.2 3,638,345 - PP_3205 0.34 +1.9 3,638,387 + PP_3205 0.38 +1.5 3,638,388 - PP_3205 0.39 +0.5 3,638,781 - PP_3205 0.78 +0.2 3,639,188 - PP_3206 0.20 +0.1 3,639,252 + PP_3206 0.27 -0.7 3,639,264 + PP_3206 0.28 -0.7 3,639,340 - PP_3206 0.36 -1.0 3,639,360 + PP_3206 0.38 -0.3 3,639,420 + PP_3206 0.44 -1.3 3,639,421 - PP_3206 0.44 -0.8 3,639,577 - PP_3206 0.60 +0.5 3,639,577 - PP_3206 0.60 +0.4 3,639,577 - PP_3206 0.60 +0.5 3,639,848 + PP_3206 0.87 +0.3 3,639,849 - PP_3206 0.87 -0.4 3,639,908 + -0.1 3,639,970 + +0.1
Or see this region's nucleotide sequence