Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2861 and PP_2862 are separated by 297 nucleotides PP_2862 and PP_2863 are separated by 28 nucleotides PP_2863 and PP_2864 are separated by 58 nucleotides PP_2864 and PP_2865 overlap by 20 nucleotides
PP_2861: PP_2861 - Methyl-accepting chemotaxis transducer, at 3,262,912 to 3,264,546
_2861
PP_2862: PP_2862 - Undecaprenyl-diphosphatase, at 3,264,844 to 3,265,677
_2862
PP_2863: PP_2863 - conserved membrane protein of unknown function, at 3,265,706 to 3,266,083
_2863
PP_2864: PP_2864 - conserved protein of unknown function, at 3,266,142 to 3,266,513
_2864
PP_2865: PP_2865 - Transcriptional regulator, LysR family, at 3,266,494 to 3,267,396
_2865
Position (kb)
3264
3265
3266 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3264.001 kb on + strand, within PP_2861 at 3264.001 kb on + strand, within PP_2861 at 3264.002 kb on - strand, within PP_2861 at 3264.002 kb on - strand, within PP_2861 at 3264.014 kb on + strand, within PP_2861 at 3264.015 kb on - strand, within PP_2861 at 3264.015 kb on - strand, within PP_2861 at 3264.015 kb on - strand, within PP_2861 at 3264.062 kb on + strand, within PP_2861 at 3264.063 kb on - strand, within PP_2861 at 3264.063 kb on - strand, within PP_2861 at 3264.110 kb on + strand, within PP_2861 at 3264.111 kb on - strand, within PP_2861 at 3264.285 kb on - strand, within PP_2861 at 3264.301 kb on + strand, within PP_2861 at 3264.302 kb on - strand, within PP_2861 at 3264.365 kb on + strand, within PP_2861 at 3264.365 kb on + strand, within PP_2861 at 3264.366 kb on - strand, within PP_2861 at 3264.368 kb on + strand, within PP_2861 at 3264.368 kb on + strand, within PP_2861 at 3264.369 kb on - strand, within PP_2861 at 3264.429 kb on - strand at 3264.619 kb on - strand at 3264.632 kb on + strand at 3264.633 kb on - strand at 3264.633 kb on - strand at 3264.643 kb on + strand at 3264.662 kb on + strand at 3264.718 kb on - strand at 3264.782 kb on - strand at 3264.939 kb on + strand, within PP_2862 at 3264.940 kb on - strand, within PP_2862 at 3265.133 kb on + strand, within PP_2862 at 3265.133 kb on + strand, within PP_2862 at 3265.158 kb on + strand, within PP_2862 at 3265.158 kb on + strand, within PP_2862 at 3265.168 kb on + strand, within PP_2862 at 3265.169 kb on - strand, within PP_2862 at 3265.450 kb on + strand, within PP_2862 at 3265.450 kb on + strand, within PP_2862 at 3265.451 kb on - strand, within PP_2862 at 3265.451 kb on - strand, within PP_2862 at 3265.459 kb on + strand, within PP_2862 at 3265.465 kb on + strand, within PP_2862 at 3265.547 kb on + strand, within PP_2862 at 3265.579 kb on - strand, within PP_2862 at 3265.579 kb on - strand, within PP_2862 at 3265.579 kb on - strand at 3265.579 kb on - strand, within PP_2862 at 3265.582 kb on + strand, within PP_2862 at 3265.583 kb on - strand, within PP_2862 at 3265.850 kb on + strand, within PP_2863 at 3265.851 kb on - strand, within PP_2863 at 3265.876 kb on + strand, within PP_2863 at 3265.876 kb on + strand, within PP_2863 at 3265.877 kb on - strand, within PP_2863 at 3265.891 kb on + strand, within PP_2863 at 3265.892 kb on - strand, within PP_2863 at 3265.892 kb on - strand, within PP_2863 at 3266.067 kb on - strand at 3266.067 kb on - strand at 3266.095 kb on - strand at 3266.187 kb on + strand, within PP_2864 at 3266.188 kb on - strand, within PP_2864 at 3266.281 kb on + strand, within PP_2864 at 3266.428 kb on + strand, within PP_2864 at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.501 kb on + strand at 3266.502 kb on - strand at 3266.502 kb on - strand at 3266.502 kb on - strand at 3266.601 kb on - strand, within PP_2865
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,264,001 + PP_2861 0.67 -0.2 3,264,001 + PP_2861 0.67 +0.1 3,264,002 - PP_2861 0.67 +0.4 3,264,002 - PP_2861 0.67 +0.5 3,264,014 + PP_2861 0.67 -0.6 3,264,015 - PP_2861 0.67 +0.1 3,264,015 - PP_2861 0.67 -0.2 3,264,015 - PP_2861 0.67 -0.1 3,264,062 + PP_2861 0.70 -0.1 3,264,063 - PP_2861 0.70 -0.0 3,264,063 - PP_2861 0.70 -1.2 3,264,110 + PP_2861 0.73 -0.0 3,264,111 - PP_2861 0.73 +0.9 3,264,285 - PP_2861 0.84 +0.4 3,264,301 + PP_2861 0.85 -0.2 3,264,302 - PP_2861 0.85 -0.8 3,264,365 + PP_2861 0.89 -0.6 3,264,365 + PP_2861 0.89 -0.9 3,264,366 - PP_2861 0.89 +0.2 3,264,368 + PP_2861 0.89 -0.7 3,264,368 + PP_2861 0.89 +0.1 3,264,369 - PP_2861 0.89 -1.2 3,264,429 - +1.4 3,264,619 - +1.2 3,264,632 + -0.9 3,264,633 - +2.0 3,264,633 - +1.8 3,264,643 + +0.0 3,264,662 + +0.2 3,264,718 - +1.1 3,264,782 - +2.0 3,264,939 + PP_2862 0.11 +1.0 3,264,940 - PP_2862 0.12 +2.0 3,265,133 + PP_2862 0.35 +2.0 3,265,133 + PP_2862 0.35 +0.6 3,265,158 + PP_2862 0.38 +2.7 3,265,158 + PP_2862 0.38 +2.2 3,265,168 + PP_2862 0.39 +2.0 3,265,169 - PP_2862 0.39 +2.5 3,265,450 + PP_2862 0.73 +1.8 3,265,450 + PP_2862 0.73 +2.7 3,265,451 - PP_2862 0.73 +1.3 3,265,451 - PP_2862 0.73 +2.5 3,265,459 + PP_2862 0.74 +2.2 3,265,465 + PP_2862 0.74 +2.9 3,265,547 + PP_2862 0.84 +2.0 3,265,579 - PP_2862 0.88 +1.3 3,265,579 - PP_2862 0.88 +1.4 3,265,579 - +0.5 3,265,579 - PP_2862 0.88 +1.3 3,265,582 + PP_2862 0.88 +2.1 3,265,583 - PP_2862 0.89 +1.0 3,265,850 + PP_2863 0.38 -0.5 3,265,851 - PP_2863 0.38 -0.6 3,265,876 + PP_2863 0.45 +2.7 3,265,876 + PP_2863 0.45 -1.0 3,265,877 - PP_2863 0.45 +0.5 3,265,891 + PP_2863 0.49 +0.7 3,265,892 - PP_2863 0.49 +1.5 3,265,892 - PP_2863 0.49 -0.8 3,266,067 - +1.4 3,266,067 - +0.6 3,266,095 - -1.7 3,266,187 + PP_2864 0.12 -0.2 3,266,188 - PP_2864 0.12 +0.9 3,266,281 + PP_2864 0.37 +0.7 3,266,428 + PP_2864 0.77 -0.1 3,266,501 + +1.9 3,266,501 + +0.2 3,266,501 + +0.6 3,266,501 + -2.1 3,266,502 - -1.3 3,266,502 - +0.5 3,266,502 - +0.3 3,266,601 - PP_2865 0.12 +1.3
Or see this region's nucleotide sequence