Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2766 and PP_2767 are separated by 9 nucleotides PP_2767 and PP_2768 overlap by 25 nucleotides PP_2768 and PP_2769 are separated by 12 nucleotides
PP_2766: PP_2766 - Long-chain-fatty-acid--CoA ligase-related protein, at 3,149,897 to 3,151,027
_2766
PP_2767: PP_2767 - putative Branched-chain amino acid ABC transporter, ATP-binding protein, at 3,151,037 to 3,151,870
_2767
PP_2768: PP_2768 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,151,846 to 3,152,727
_2768
PP_2769: PP_2769 - putative Branched-chain amino acid ABC transporter, permease protein, at 3,152,740 to 3,153,729
_2769
Position (kb)
3151
3152
3153 Strain fitness (log2 ratio)
-1
0
1 at 3150.937 kb on + strand at 3151.505 kb on + strand, within PP_2767 at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.897 kb on + strand at 3151.898 kb on - strand at 3151.922 kb on + strand at 3151.931 kb on - strand at 3151.931 kb on - strand at 3151.974 kb on - strand, within PP_2768 at 3152.152 kb on + strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.153 kb on - strand, within PP_2768 at 3152.542 kb on - strand, within PP_2768 at 3152.609 kb on - strand, within PP_2768 at 3152.654 kb on - strand at 3152.909 kb on + strand, within PP_2769 at 3152.910 kb on - strand, within PP_2769 at 3152.910 kb on - strand, within PP_2769 at 3152.996 kb on + strand, within PP_2769 at 3153.013 kb on + strand, within PP_2769 at 3153.014 kb on - strand, within PP_2769 at 3153.015 kb on + strand, within PP_2769 at 3153.133 kb on + strand, within PP_2769 at 3153.134 kb on - strand, within PP_2769 at 3153.377 kb on - strand, within PP_2769 at 3153.412 kb on + strand, within PP_2769 at 3153.413 kb on - strand, within PP_2769 at 3153.481 kb on - strand, within PP_2769 at 3153.603 kb on - strand, within PP_2769 at 3153.603 kb on - strand, within PP_2769
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,150,937 + -0.6 3,151,505 + PP_2767 0.56 +0.1 3,151,897 + +0.3 3,151,897 + +0.1 3,151,897 + +0.3 3,151,897 + +0.0 3,151,898 - -1.1 3,151,922 + -1.2 3,151,931 - -0.8 3,151,931 - +1.0 3,151,974 - PP_2768 0.15 +1.5 3,152,152 + PP_2768 0.35 -0.2 3,152,153 - PP_2768 0.35 +0.2 3,152,153 - PP_2768 0.35 +1.2 3,152,153 - PP_2768 0.35 +0.3 3,152,542 - PP_2768 0.79 +0.2 3,152,609 - PP_2768 0.87 +0.7 3,152,654 - +0.0 3,152,909 + PP_2769 0.17 -0.3 3,152,910 - PP_2769 0.17 -0.1 3,152,910 - PP_2769 0.17 +0.9 3,152,996 + PP_2769 0.26 +0.1 3,153,013 + PP_2769 0.28 +0.0 3,153,014 - PP_2769 0.28 -1.3 3,153,015 + PP_2769 0.28 +1.5 3,153,133 + PP_2769 0.40 +0.5 3,153,134 - PP_2769 0.40 +0.1 3,153,377 - PP_2769 0.64 -0.1 3,153,412 + PP_2769 0.68 -0.1 3,153,413 - PP_2769 0.68 -0.4 3,153,481 - PP_2769 0.75 -0.5 3,153,603 - PP_2769 0.87 +0.6 3,153,603 - PP_2769 0.87 -0.0
Or see this region's nucleotide sequence