Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2736 and PP_2737 are separated by 6 nucleotides PP_2737 and PP_2738 overlap by 4 nucleotides PP_2738 and PP_2739 are separated by 103 nucleotides
PP_2736: PP_2736 - conserved protein of unknown function, at 3,119,437 to 3,120,684
_2736
PP_2737: PP_2737 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 3,120,691 to 3,121,443
_2737
PP_2738: PP_2738 - putative Transcriptional regulator, at 3,121,440 to 3,121,862
_2738
PP_2739: PP_2739 - putative Sensory box protein, at 3,121,966 to 3,122,421
_2739
Position (kb)
3120
3121
3122 Strain fitness (log2 ratio)
-2
-1
0
1 at 3119.832 kb on - strand, within PP_2736 at 3120.045 kb on - strand, within PP_2736 at 3120.194 kb on - strand, within PP_2736 at 3120.194 kb on - strand, within PP_2736 at 3120.200 kb on + strand, within PP_2736 at 3120.201 kb on - strand, within PP_2736 at 3120.201 kb on - strand, within PP_2736 at 3120.201 kb on - strand, within PP_2736 at 3120.201 kb on - strand, within PP_2736 at 3120.396 kb on + strand, within PP_2736 at 3120.398 kb on + strand, within PP_2736 at 3120.398 kb on + strand, within PP_2736 at 3120.494 kb on + strand, within PP_2736 at 3120.495 kb on - strand, within PP_2736 at 3120.526 kb on - strand, within PP_2736 at 3120.529 kb on + strand, within PP_2736 at 3120.530 kb on - strand, within PP_2736 at 3120.530 kb on - strand, within PP_2736 at 3120.530 kb on - strand, within PP_2736 at 3120.530 kb on - strand, within PP_2736 at 3120.677 kb on - strand at 3120.678 kb on + strand at 3120.679 kb on - strand at 3120.679 kb on - strand at 3120.831 kb on + strand, within PP_2737 at 3121.182 kb on - strand, within PP_2737 at 3121.407 kb on + strand at 3121.498 kb on + strand, within PP_2738 at 3121.637 kb on - strand, within PP_2738 at 3121.678 kb on + strand, within PP_2738 at 3121.703 kb on - strand, within PP_2738 at 3121.718 kb on - strand, within PP_2738 at 3121.718 kb on - strand, within PP_2738 at 3121.743 kb on + strand, within PP_2738 at 3121.808 kb on + strand, within PP_2738 at 3121.848 kb on + strand at 3121.848 kb on + strand at 3121.848 kb on + strand at 3121.853 kb on - strand at 3121.862 kb on + strand at 3121.862 kb on + strand at 3121.862 kb on + strand at 3121.863 kb on - strand at 3121.863 kb on - strand at 3121.908 kb on + strand at 3122.078 kb on + strand, within PP_2739 at 3122.078 kb on + strand, within PP_2739 at 3122.079 kb on - strand, within PP_2739 at 3122.079 kb on - strand, within PP_2739 at 3122.081 kb on + strand, within PP_2739 at 3122.081 kb on + strand, within PP_2739 at 3122.081 kb on + strand, within PP_2739 at 3122.082 kb on - strand, within PP_2739 at 3122.082 kb on - strand, within PP_2739 at 3122.156 kb on + strand, within PP_2739 at 3122.272 kb on - strand, within PP_2739 at 3122.272 kb on - strand, within PP_2739 at 3122.318 kb on + strand, within PP_2739 at 3122.318 kb on + strand, within PP_2739 at 3122.318 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.322 kb on + strand, within PP_2739 at 3122.323 kb on - strand, within PP_2739 at 3122.323 kb on - strand, within PP_2739 at 3122.424 kb on - strand at 3122.424 kb on - strand at 3122.424 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 3,119,832 - PP_2736 0.32 +1.2 3,120,045 - PP_2736 0.49 -0.4 3,120,194 - PP_2736 0.61 -1.3 3,120,194 - PP_2736 0.61 +0.6 3,120,200 + PP_2736 0.61 -0.9 3,120,201 - PP_2736 0.61 +0.7 3,120,201 - PP_2736 0.61 -0.2 3,120,201 - PP_2736 0.61 -1.2 3,120,201 - PP_2736 0.61 -1.2 3,120,396 + PP_2736 0.77 +0.3 3,120,398 + PP_2736 0.77 +0.7 3,120,398 + PP_2736 0.77 -0.3 3,120,494 + PP_2736 0.85 +0.7 3,120,495 - PP_2736 0.85 +0.0 3,120,526 - PP_2736 0.87 -0.6 3,120,529 + PP_2736 0.88 -0.1 3,120,530 - PP_2736 0.88 +1.0 3,120,530 - PP_2736 0.88 +0.2 3,120,530 - PP_2736 0.88 +1.4 3,120,530 - PP_2736 0.88 -1.0 3,120,677 - -0.4 3,120,678 + -0.8 3,120,679 - -1.8 3,120,679 - -0.1 3,120,831 + PP_2737 0.19 +1.1 3,121,182 - PP_2737 0.65 +0.3 3,121,407 + +0.5 3,121,498 + PP_2738 0.14 +1.5 3,121,637 - PP_2738 0.47 +0.6 3,121,678 + PP_2738 0.56 -0.6 3,121,703 - PP_2738 0.62 -0.6 3,121,718 - PP_2738 0.66 -0.6 3,121,718 - PP_2738 0.66 -1.0 3,121,743 + PP_2738 0.72 -0.8 3,121,808 + PP_2738 0.87 -0.2 3,121,848 + -0.9 3,121,848 + +0.2 3,121,848 + -0.1 3,121,853 - +0.7 3,121,862 + -0.3 3,121,862 + -0.5 3,121,862 + -0.2 3,121,863 - -0.1 3,121,863 - -1.8 3,121,908 + -1.5 3,122,078 + PP_2739 0.25 -0.3 3,122,078 + PP_2739 0.25 -0.9 3,122,079 - PP_2739 0.25 -0.2 3,122,079 - PP_2739 0.25 -1.4 3,122,081 + PP_2739 0.25 -0.1 3,122,081 + PP_2739 0.25 +0.8 3,122,081 + PP_2739 0.25 +0.1 3,122,082 - PP_2739 0.25 -0.4 3,122,082 - PP_2739 0.25 +0.4 3,122,156 + PP_2739 0.42 +1.0 3,122,272 - PP_2739 0.67 +0.1 3,122,272 - PP_2739 0.67 +0.3 3,122,318 + PP_2739 0.77 +0.4 3,122,318 + PP_2739 0.77 +0.4 3,122,318 + PP_2739 0.77 -1.4 3,122,322 + PP_2739 0.78 -0.1 3,122,322 + PP_2739 0.78 +0.5 3,122,322 + PP_2739 0.78 +1.3 3,122,322 + PP_2739 0.78 -2.2 3,122,322 + PP_2739 0.78 +0.2 3,122,322 + PP_2739 0.78 -0.0 3,122,323 - PP_2739 0.78 +0.9 3,122,323 - PP_2739 0.78 +0.1 3,122,424 - -0.4 3,122,424 - -0.3 3,122,424 - +0.9
Or see this region's nucleotide sequence