Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2431 and PP_2432 are separated by 103 nucleotides PP_2432 and PP_2433 are separated by 112 nucleotides PP_2433 and PP_2434 overlap by 4 nucleotides PP_2434 and PP_2435 are separated by 7 nucleotides
PP_2431: PP_2431 - Transcriptional regulator, LysR family, at 2,778,434 to 2,779,357
_2431
PP_2432: PP_2432 - 6,7-dihydropteridine reductase, at 2,779,461 to 2,780,114
_2432
PP_2433: PP_2433 - conserved protein of unknown function, at 2,780,227 to 2,780,706
_2433
PP_2434: PP_2434 - conserved protein of unknown function, at 2,780,703 to 2,781,140
_2434
PP_2435: PP_2435 - cysteine desulfurase, at 2,781,148 to 2,782,365
_2435
Position (kb)
2780
2781 Strain fitness (log2 ratio)
-1
0
1
2 at 2779.384 kb on + strand at 2779.384 kb on + strand at 2779.419 kb on + strand at 2779.419 kb on + strand at 2779.471 kb on + strand at 2779.491 kb on + strand at 2779.492 kb on - strand at 2779.507 kb on - strand at 2779.669 kb on - strand, within PP_2432 at 2779.830 kb on + strand, within PP_2432 at 2779.830 kb on + strand, within PP_2432 at 2779.831 kb on - strand, within PP_2432 at 2779.891 kb on - strand, within PP_2432 at 2779.905 kb on - strand, within PP_2432 at 2779.905 kb on - strand, within PP_2432 at 2780.287 kb on + strand, within PP_2433 at 2780.396 kb on + strand, within PP_2433 at 2780.396 kb on + strand, within PP_2433 at 2780.727 kb on + strand at 2780.728 kb on - strand at 2780.761 kb on + strand, within PP_2434 at 2780.762 kb on - strand, within PP_2434 at 2780.762 kb on - strand, within PP_2434 at 2780.762 kb on - strand, within PP_2434 at 2780.799 kb on + strand, within PP_2434 at 2780.799 kb on + strand, within PP_2434 at 2780.800 kb on - strand, within PP_2434 at 2780.800 kb on - strand, within PP_2434 at 2780.800 kb on - strand, within PP_2434 at 2780.874 kb on - strand, within PP_2434 at 2781.057 kb on + strand, within PP_2434 at 2781.057 kb on + strand, within PP_2434 at 2781.057 kb on + strand, within PP_2434 at 2781.057 kb on + strand, within PP_2434 at 2781.138 kb on + strand at 2781.139 kb on - strand at 2781.653 kb on + strand, within PP_2435 at 2781.653 kb on + strand, within PP_2435 at 2781.654 kb on - strand, within PP_2435 at 2781.654 kb on - strand, within PP_2435 at 2781.654 kb on - strand, within PP_2435 at 2781.681 kb on - strand, within PP_2435
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,779,384 + +0.2 2,779,384 + -1.4 2,779,419 + +1.2 2,779,419 + +1.1 2,779,471 + -0.4 2,779,491 + +0.2 2,779,492 - +0.4 2,779,507 - +0.1 2,779,669 - PP_2432 0.32 +0.6 2,779,830 + PP_2432 0.56 -0.5 2,779,830 + PP_2432 0.56 -0.0 2,779,831 - PP_2432 0.57 +0.2 2,779,891 - PP_2432 0.66 +0.5 2,779,905 - PP_2432 0.68 +0.1 2,779,905 - PP_2432 0.68 -0.9 2,780,287 + PP_2433 0.12 +1.0 2,780,396 + PP_2433 0.35 +0.8 2,780,396 + PP_2433 0.35 +1.9 2,780,727 + +0.9 2,780,728 - +2.6 2,780,761 + PP_2434 0.13 -0.1 2,780,762 - PP_2434 0.13 +0.4 2,780,762 - PP_2434 0.13 +0.4 2,780,762 - PP_2434 0.13 -0.7 2,780,799 + PP_2434 0.22 +0.2 2,780,799 + PP_2434 0.22 -0.7 2,780,800 - PP_2434 0.22 -1.6 2,780,800 - PP_2434 0.22 +1.7 2,780,800 - PP_2434 0.22 -0.4 2,780,874 - PP_2434 0.39 -0.8 2,781,057 + PP_2434 0.81 +0.4 2,781,057 + PP_2434 0.81 -1.3 2,781,057 + PP_2434 0.81 -0.5 2,781,057 + PP_2434 0.81 -0.6 2,781,138 + +0.2 2,781,139 - -0.1 2,781,653 + PP_2435 0.41 +0.1 2,781,653 + PP_2435 0.41 +1.0 2,781,654 - PP_2435 0.42 +1.0 2,781,654 - PP_2435 0.42 -0.1 2,781,654 - PP_2435 0.42 +1.1 2,781,681 - PP_2435 0.44 -0.1
Or see this region's nucleotide sequence