Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2134 and PP_2136 are separated by 658 nucleotides PP_2136 and PP_2137 are separated by 31 nucleotides
PP_2134: PP_2134 - transposase, at 2,435,457 to 2,436,422
_2134
PP_2136: PP_2136 - enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase, at 2,437,081 to 2,439,228
_2136
PP_2137: PP_2137 - beta-ketoadipyl CoA thiolase, beta component, at 2,439,260 to 2,440,435
_2137
Position (kb)
2437
2438
2439
2440 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2436.562 kb on + strand at 2436.605 kb on + strand at 2436.605 kb on + strand at 2436.605 kb on + strand at 2436.702 kb on + strand at 2436.702 kb on + strand at 2436.726 kb on + strand at 2436.727 kb on - strand at 2436.727 kb on - strand at 2436.761 kb on + strand at 2436.762 kb on - strand at 2436.762 kb on - strand at 2436.762 kb on - strand at 2436.950 kb on + strand at 2436.950 kb on + strand at 2436.951 kb on - strand at 2436.951 kb on - strand at 2436.971 kb on - strand at 2436.971 kb on - strand at 2436.971 kb on - strand at 2436.981 kb on - strand at 2437.017 kb on - strand at 2437.017 kb on - strand at 2437.047 kb on + strand at 2437.048 kb on - strand at 2437.087 kb on + strand at 2437.087 kb on + strand at 2437.095 kb on + strand at 2437.516 kb on + strand, within PP_2136 at 2437.516 kb on + strand, within PP_2136 at 2437.714 kb on + strand, within PP_2136 at 2437.714 kb on + strand, within PP_2136 at 2438.071 kb on + strand, within PP_2136 at 2438.312 kb on + strand, within PP_2136 at 2438.522 kb on - strand, within PP_2136 at 2438.695 kb on + strand, within PP_2136 at 2438.695 kb on + strand, within PP_2136 at 2438.838 kb on + strand, within PP_2136 at 2438.848 kb on + strand, within PP_2136 at 2438.848 kb on + strand, within PP_2136 at 2438.849 kb on - strand, within PP_2136 at 2438.849 kb on - strand, within PP_2136 at 2439.058 kb on + strand at 2439.148 kb on + strand at 2439.149 kb on - strand at 2439.149 kb on - strand at 2439.242 kb on + strand at 2439.243 kb on - strand at 2439.243 kb on - strand at 2439.243 kb on - strand at 2439.260 kb on - strand at 2439.660 kb on + strand, within PP_2137 at 2439.660 kb on + strand, within PP_2137 at 2439.661 kb on - strand, within PP_2137 at 2439.854 kb on + strand, within PP_2137 at 2439.980 kb on - strand, within PP_2137
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,436,562 + +0.7 2,436,605 + +1.0 2,436,605 + -0.3 2,436,605 + -0.1 2,436,702 + -1.5 2,436,702 + +1.4 2,436,726 + +0.9 2,436,727 - -1.4 2,436,727 - +1.5 2,436,761 + +0.2 2,436,762 - +0.2 2,436,762 - +0.3 2,436,762 - +0.1 2,436,950 + +0.7 2,436,950 + +0.3 2,436,951 - +1.1 2,436,951 - +1.7 2,436,971 - +0.3 2,436,971 - +0.4 2,436,971 - -1.4 2,436,981 - +2.4 2,437,017 - +0.8 2,437,017 - -0.2 2,437,047 + +0.6 2,437,048 - +2.9 2,437,087 + +0.3 2,437,087 + -1.2 2,437,095 + +2.0 2,437,516 + PP_2136 0.20 -0.5 2,437,516 + PP_2136 0.20 +3.3 2,437,714 + PP_2136 0.29 +0.7 2,437,714 + PP_2136 0.29 +1.6 2,438,071 + PP_2136 0.46 +2.5 2,438,312 + PP_2136 0.57 +1.6 2,438,522 - PP_2136 0.67 +1.5 2,438,695 + PP_2136 0.75 +1.9 2,438,695 + PP_2136 0.75 +4.1 2,438,838 + PP_2136 0.82 +1.7 2,438,848 + PP_2136 0.82 +0.9 2,438,848 + PP_2136 0.82 +1.4 2,438,849 - PP_2136 0.82 +1.6 2,438,849 - PP_2136 0.82 +2.2 2,439,058 + +1.5 2,439,148 + +1.1 2,439,149 - -0.2 2,439,149 - +2.0 2,439,242 + +0.4 2,439,243 - +0.4 2,439,243 - +0.3 2,439,243 - -0.5 2,439,260 - +0.9 2,439,660 + PP_2137 0.34 +2.2 2,439,660 + PP_2137 0.34 +0.7 2,439,661 - PP_2137 0.34 +2.8 2,439,854 + PP_2137 0.51 +1.1 2,439,980 - PP_2137 0.61 +1.0
Or see this region's nucleotide sequence