Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2077 and PP_2078 are separated by 123 nucleotides PP_2078 and PP_2079 are separated by 83 nucleotides PP_2079 and PP_2080 are separated by 170 nucleotides
PP_2077: PP_2077 - diaminopimelate decarboxylase, at 2,364,209 to 2,365,441
_2077
PP_2078: PP_2078 - transcriptional activator protein LysR, at 2,365,565 to 2,366,470
_2078
PP_2079: PP_2079 - conserved protein of unknown function, at 2,366,554 to 2,367,012
_2079
PP_2080: PP_2080 - NAD-specific glutamate dehydrogenase, at 2,367,183 to 2,372,048
_2080
Position (kb)
2365
2366
2367 Strain fitness (log2 ratio)
-1
0
1
2 at 2364.578 kb on - strand, within PP_2077 at 2364.589 kb on + strand, within PP_2077 at 2364.589 kb on + strand, within PP_2077 at 2364.590 kb on - strand, within PP_2077 at 2364.633 kb on + strand, within PP_2077 at 2364.634 kb on - strand, within PP_2077 at 2364.876 kb on + strand, within PP_2077 at 2365.322 kb on + strand at 2365.322 kb on + strand at 2365.322 kb on + strand at 2365.322 kb on + strand at 2365.322 kb on + strand at 2365.377 kb on + strand at 2365.377 kb on + strand at 2365.378 kb on - strand at 2365.378 kb on - strand at 2365.386 kb on - strand at 2365.386 kb on - strand at 2365.456 kb on + strand at 2365.456 kb on + strand at 2365.456 kb on + strand at 2365.456 kb on + strand at 2365.457 kb on - strand at 2365.457 kb on - strand at 2365.457 kb on - strand at 2365.457 kb on - strand at 2365.458 kb on + strand at 2365.458 kb on + strand at 2365.459 kb on - strand at 2365.459 kb on - strand at 2365.459 kb on - strand at 2365.535 kb on - strand at 2365.559 kb on - strand at 2365.784 kb on + strand, within PP_2078 at 2366.036 kb on + strand, within PP_2078 at 2366.138 kb on + strand, within PP_2078 at 2366.165 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.377 kb on - strand, within PP_2078 at 2366.504 kb on - strand at 2366.545 kb on - strand at 2366.545 kb on - strand at 2366.657 kb on - strand, within PP_2079 at 2366.733 kb on + strand at 2366.734 kb on - strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.965 kb on + strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2366.966 kb on - strand, within PP_2079 at 2367.016 kb on + strand at 2367.016 kb on + strand at 2367.016 kb on + strand at 2367.017 kb on - strand at 2367.088 kb on - strand at 2367.154 kb on + strand at 2367.176 kb on - strand at 2367.176 kb on - strand at 2367.420 kb on + strand at 2367.420 kb on + strand at 2367.421 kb on - strand at 2367.421 kb on - strand at 2367.432 kb on + strand at 2367.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,364,578 - PP_2077 0.30 +1.3 2,364,589 + PP_2077 0.31 -1.0 2,364,589 + PP_2077 0.31 -1.0 2,364,590 - PP_2077 0.31 +0.9 2,364,633 + PP_2077 0.34 -0.7 2,364,634 - PP_2077 0.34 +2.7 2,364,876 + PP_2077 0.54 +0.7 2,365,322 + -0.4 2,365,322 + +0.2 2,365,322 + -1.0 2,365,322 + -1.6 2,365,322 + -0.3 2,365,377 + -0.1 2,365,377 + +0.3 2,365,378 - -0.8 2,365,378 - +0.1 2,365,386 - -0.4 2,365,386 - +0.1 2,365,456 + -0.1 2,365,456 + +0.5 2,365,456 + +0.3 2,365,456 + -0.2 2,365,457 - -0.5 2,365,457 - -0.3 2,365,457 - +1.0 2,365,457 - -0.5 2,365,458 + -0.1 2,365,458 + +0.2 2,365,459 - +0.3 2,365,459 - -0.3 2,365,459 - +0.3 2,365,535 - +0.7 2,365,559 - -0.2 2,365,784 + PP_2078 0.24 -0.6 2,366,036 + PP_2078 0.52 +0.2 2,366,138 + PP_2078 0.63 +1.0 2,366,165 - PP_2078 0.66 -0.2 2,366,377 - PP_2078 0.90 +0.9 2,366,377 - PP_2078 0.90 +0.8 2,366,377 - PP_2078 0.90 +1.3 2,366,504 - +0.4 2,366,545 - -0.8 2,366,545 - -0.5 2,366,657 - PP_2079 0.22 +0.1 2,366,733 + +2.0 2,366,734 - PP_2079 0.39 +0.5 2,366,965 + PP_2079 0.90 -1.7 2,366,965 + PP_2079 0.90 +0.1 2,366,965 + PP_2079 0.90 -0.9 2,366,965 + PP_2079 0.90 -0.8 2,366,966 - PP_2079 0.90 -0.4 2,366,966 - PP_2079 0.90 +0.4 2,366,966 - PP_2079 0.90 -0.1 2,367,016 + +1.7 2,367,016 + +0.9 2,367,016 + -1.2 2,367,017 - -0.8 2,367,088 - +0.8 2,367,154 + +1.8 2,367,176 - -1.0 2,367,176 - -0.1 2,367,420 + +1.2 2,367,420 + +0.6 2,367,421 - +0.2 2,367,421 - -0.5 2,367,432 + -0.5 2,367,433 - -1.2
Or see this region's nucleotide sequence