Strain Fitness in Pseudomonas putida KT2440 around PP_2018

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
2,289,726	-		PP_2017	0.76	+1.0
2,289,761	+		PP_2017	0.77	+1.5
2,289,761	+		PP_2017	0.77	+0.4
2,289,762	-		PP_2017	0.77	-0.8
2,289,762	-		PP_2017	0.77	+0.7
2,289,818	+		PP_2017	0.79	-2.1
2,289,847	-		PP_2017	0.80	+0.3
2,289,847	-		PP_2017	0.80	-0.9
2,289,915	+		PP_2017	0.83	-0.1
2,290,268	+				+0.0
2,290,268	+				+0.0
2,290,268	+				-0.2
2,290,347	-				-0.7
2,290,347	-				-1.8
2,290,347	-				+1.4
2,290,347	-				+1.4
2,290,373	+				+1.1
2,290,433	+				+2.5
2,290,433	+				-0.9
2,290,434	-				-0.0
2,290,434	-				-0.0
2,290,434	-				-1.7
2,290,451	-				+0.2
2,290,471	+				+3.5
2,290,487	+				+0.1
2,290,487	+				-0.5
2,290,488	-				+1.1
2,290,501	-				+1.0
2,290,501	-				+0.7
2,290,582	+				-0.4
2,290,582	+				+0.4
2,290,582	+				+0.1
2,290,583	-				-1.0
2,290,586	+				+0.5
2,290,586	+				+0.6
2,290,587	-				+1.0
2,290,587	-				-0.5
2,290,587	-				-0.2
2,290,587	-				-0.6
2,290,689	+				+0.5
2,290,690	-				-2.3
2,290,808	+		PP_2018	0.16	+0.0
2,291,025	+		PP_2018	0.39	-0.3
2,291,025	+		PP_2018	0.39	+0.1
2,291,025	+		PP_2018	0.39	+2.3
2,291,025	+		PP_2018	0.39	+2.7
2,291,026	-		PP_2018	0.39	+1.5
2,291,026	-		PP_2018	0.39	+0.3
2,291,385	-		PP_2018	0.77	+0.3
2,291,473	-		PP_2018	0.86	-0.0
2,291,626	+				+0.2
2,291,626	+				-0.2
2,291,626	+				+0.2
2,291,627	-				+0.0
2,291,627	-				-0.7
2,291,627	-				-2.1
2,291,627	-				+0.3
2,291,627	-				-0.7
2,291,627	-				+0.2
2,291,900	+		PP_2019	0.12	-0.2
2,291,900	+		PP_2019	0.12	+1.5
2,291,900	+		PP_2019	0.12	+0.5
2,291,901	-		PP_2019	0.12	+0.0
2,291,901	-		PP_2019	0.12	+1.5
2,291,901	-		PP_2019	0.12	+0.5
2,291,901	-		PP_2019	0.12	+1.5
2,291,901	-		PP_2019	0.12	+0.5
2,291,901	-		PP_2019	0.12	-2.1
2,291,939	+		PP_2019	0.14	-0.1
2,291,939	+		PP_2019	0.14	-0.0
2,291,940	-		PP_2019	0.14	+0.4
2,291,940	-		PP_2019	0.14	-1.6
2,292,044	-		PP_2019	0.18	+0.7
2,292,051	-		PP_2019	0.19	-0.3
2,292,173	+		PP_2019	0.24	-0.1
2,292,173	+		PP_2019	0.24	+0.9
2,292,174	-		PP_2019	0.24	+0.4
2,292,215	+		PP_2019	0.25	-3.0
2,292,215	+		PP_2019	0.25	+0.0
2,292,216	-		PP_2019	0.25	+0.6
2,292,392	+		PP_2019	0.33	+0.8
2,292,393	-		PP_2019	0.33	-0.1
2,292,483	+		PP_2019	0.37	-0.6