Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1925 and PP_1926 are separated by 25 nucleotides PP_1926 and PP_1927 are separated by 10 nucleotides PP_1927 and PP_1928 are separated by 11 nucleotides
PP_1925: PP_1925 - putative Monooxygenase, at 2,172,421 to 2,173,503
_1925
PP_1926: PP_1926 - putative phosphotyrosine-protein phosphatase, at 2,173,529 to 2,174,026
_1926
PP_1927: PP_1927 - putative Arsenical resistance protein ArsH, at 2,174,037 to 2,174,762
_1927
PP_1928: PP_1928 - arsenate reductase, at 2,174,774 to 2,175,262
_1928
Position (kb)
2173
2174
2175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2172.567 kb on + strand, within PP_1925 at 2172.568 kb on - strand, within PP_1925 at 2172.655 kb on - strand, within PP_1925 at 2172.736 kb on + strand, within PP_1925 at 2172.792 kb on + strand, within PP_1925 at 2173.031 kb on + strand, within PP_1925 at 2173.032 kb on - strand, within PP_1925 at 2173.032 kb on - strand, within PP_1925 at 2173.079 kb on + strand, within PP_1925 at 2173.091 kb on + strand, within PP_1925 at 2173.092 kb on - strand, within PP_1925 at 2173.160 kb on - strand, within PP_1925 at 2173.215 kb on + strand, within PP_1925 at 2173.238 kb on + strand, within PP_1925 at 2173.238 kb on + strand, within PP_1925 at 2173.238 kb on + strand, within PP_1925 at 2173.238 kb on + strand, within PP_1925 at 2173.239 kb on - strand, within PP_1925 at 2173.239 kb on - strand, within PP_1925 at 2173.250 kb on + strand, within PP_1925 at 2173.250 kb on + strand, within PP_1925 at 2173.250 kb on + strand, within PP_1925 at 2173.251 kb on - strand, within PP_1925 at 2173.279 kb on - strand, within PP_1925 at 2173.555 kb on - strand at 2173.555 kb on - strand at 2173.555 kb on - strand at 2173.555 kb on - strand at 2173.555 kb on - strand at 2173.555 kb on - strand at 2173.888 kb on + strand, within PP_1926 at 2173.888 kb on + strand, within PP_1926 at 2173.889 kb on - strand, within PP_1926 at 2173.889 kb on - strand, within PP_1926 at 2173.922 kb on - strand, within PP_1926 at 2173.922 kb on - strand, within PP_1926 at 2173.980 kb on - strand at 2174.034 kb on - strand at 2174.043 kb on - strand at 2174.067 kb on + strand at 2174.067 kb on + strand at 2174.098 kb on + strand at 2174.106 kb on + strand at 2174.107 kb on - strand at 2174.107 kb on - strand at 2174.113 kb on + strand, within PP_1927 at 2174.114 kb on - strand, within PP_1927 at 2174.114 kb on - strand, within PP_1927 at 2174.267 kb on + strand, within PP_1927 at 2174.267 kb on + strand, within PP_1927 at 2174.267 kb on + strand, within PP_1927 at 2174.267 kb on + strand, within PP_1927 at 2174.268 kb on - strand, within PP_1927 at 2174.268 kb on - strand, within PP_1927 at 2174.268 kb on - strand, within PP_1927 at 2174.268 kb on - strand, within PP_1927 at 2174.309 kb on + strand, within PP_1927 at 2174.379 kb on + strand, within PP_1927 at 2174.380 kb on - strand, within PP_1927 at 2174.556 kb on - strand, within PP_1927 at 2174.642 kb on - strand, within PP_1927 at 2174.642 kb on - strand, within PP_1927 at 2174.775 kb on + strand at 2174.776 kb on - strand at 2174.776 kb on - strand at 2174.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 2,172,567 + PP_1925 0.13 -0.6 2,172,568 - PP_1925 0.14 +0.6 2,172,655 - PP_1925 0.22 -0.3 2,172,736 + PP_1925 0.29 +0.8 2,172,792 + PP_1925 0.34 +0.4 2,173,031 + PP_1925 0.56 -0.4 2,173,032 - PP_1925 0.56 -0.1 2,173,032 - PP_1925 0.56 -1.0 2,173,079 + PP_1925 0.61 +0.2 2,173,091 + PP_1925 0.62 -0.3 2,173,092 - PP_1925 0.62 +0.8 2,173,160 - PP_1925 0.68 -0.1 2,173,215 + PP_1925 0.73 +0.1 2,173,238 + PP_1925 0.75 +0.3 2,173,238 + PP_1925 0.75 +0.5 2,173,238 + PP_1925 0.75 +0.4 2,173,238 + PP_1925 0.75 +0.7 2,173,239 - PP_1925 0.76 +0.5 2,173,239 - PP_1925 0.76 +0.2 2,173,250 + PP_1925 0.77 -2.5 2,173,250 + PP_1925 0.77 +0.9 2,173,250 + PP_1925 0.77 -1.2 2,173,251 - PP_1925 0.77 +2.3 2,173,279 - PP_1925 0.79 +0.6 2,173,555 - -0.1 2,173,555 - +0.6 2,173,555 - -2.0 2,173,555 - -3.4 2,173,555 - -0.6 2,173,555 - -0.5 2,173,888 + PP_1926 0.72 +0.6 2,173,888 + PP_1926 0.72 +0.3 2,173,889 - PP_1926 0.72 +1.3 2,173,889 - PP_1926 0.72 -0.9 2,173,922 - PP_1926 0.79 -0.5 2,173,922 - PP_1926 0.79 +3.7 2,173,980 - -1.1 2,174,034 - +0.8 2,174,043 - -0.6 2,174,067 + -0.7 2,174,067 + -0.7 2,174,098 + +0.9 2,174,106 + -0.5 2,174,107 - +0.2 2,174,107 - -1.0 2,174,113 + PP_1927 0.10 +0.4 2,174,114 - PP_1927 0.11 -0.2 2,174,114 - PP_1927 0.11 +1.1 2,174,267 + PP_1927 0.32 -1.5 2,174,267 + PP_1927 0.32 -1.2 2,174,267 + PP_1927 0.32 -0.9 2,174,267 + PP_1927 0.32 +1.1 2,174,268 - PP_1927 0.32 +0.3 2,174,268 - PP_1927 0.32 -0.2 2,174,268 - PP_1927 0.32 -1.2 2,174,268 - PP_1927 0.32 -0.2 2,174,309 + PP_1927 0.37 +0.2 2,174,379 + PP_1927 0.47 -0.4 2,174,380 - PP_1927 0.47 +0.3 2,174,556 - PP_1927 0.71 +0.4 2,174,642 - PP_1927 0.83 +0.7 2,174,642 - PP_1927 0.83 +0.2 2,174,775 + -0.5 2,174,776 - -0.8 2,174,776 - -0.6 2,174,776 - -3.1
Or see this region's nucleotide sequence