Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1120 and PP_1121 are separated by 159 nucleotides PP_1121 and PP_1122 are separated by 14 nucleotides PP_1122 and PP_1123 are separated by 98 nucleotides PP_1123 and PP_1124 are separated by 116 nucleotides
PP_1120: PP_1120 - conserved protein of unknown function, at 1,283,409 to 1,283,903
_1120
PP_1121: PP_1121 - OmpA family protein, at 1,284,063 to 1,284,755
_1121
PP_1122: PP_1122 - OmpA family protein, at 1,284,770 to 1,285,564
_1122
PP_1123: PP_1123 - conserved protein of unknown function, at 1,285,663 to 1,285,938
_1123
PP_1124: PP_1124 - conserved membrane protein of unknown function, at 1,286,055 to 1,286,519
_1124
Position (kb)
1284
1285
1286 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1283.813 kb on + strand, within PP_1120 at 1283.813 kb on + strand, within PP_1120 at 1283.818 kb on - strand, within PP_1120 at 1283.977 kb on + strand at 1283.977 kb on + strand at 1283.977 kb on + strand at 1283.977 kb on + strand at 1283.978 kb on - strand at 1283.978 kb on - strand at 1283.978 kb on - strand at 1283.982 kb on + strand at 1283.982 kb on + strand at 1283.982 kb on + strand at 1283.983 kb on - strand at 1283.983 kb on - strand at 1284.013 kb on + strand at 1284.013 kb on + strand at 1284.013 kb on + strand at 1284.070 kb on + strand at 1284.070 kb on + strand at 1284.071 kb on - strand at 1284.071 kb on - strand at 1284.071 kb on - strand at 1284.071 kb on - strand at 1284.073 kb on + strand at 1284.073 kb on + strand at 1284.073 kb on + strand at 1284.073 kb on + strand at 1284.073 kb on + strand at 1284.074 kb on - strand at 1284.074 kb on - strand at 1284.094 kb on + strand at 1284.173 kb on - strand, within PP_1121 at 1284.173 kb on - strand, within PP_1121 at 1284.173 kb on - strand, within PP_1121 at 1284.173 kb on - strand, within PP_1121 at 1284.173 kb on - strand, within PP_1121 at 1284.255 kb on + strand, within PP_1121 at 1284.255 kb on + strand, within PP_1121 at 1284.489 kb on - strand, within PP_1121 at 1284.534 kb on - strand, within PP_1121 at 1284.534 kb on - strand, within PP_1121 at 1284.573 kb on + strand, within PP_1121 at 1284.574 kb on - strand, within PP_1121 at 1284.574 kb on - strand, within PP_1121 at 1284.574 kb on - strand, within PP_1121 at 1284.574 kb on - strand, within PP_1121 at 1284.732 kb on - strand at 1284.753 kb on + strand at 1285.068 kb on - strand, within PP_1122 at 1285.388 kb on + strand, within PP_1122 at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.649 kb on + strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.650 kb on - strand at 1285.662 kb on + strand at 1286.266 kb on + strand, within PP_1124 at 1286.267 kb on - strand, within PP_1124 at 1286.267 kb on - strand, within PP_1124 at 1286.322 kb on + strand, within PP_1124 at 1286.322 kb on + strand, within PP_1124 at 1286.469 kb on + strand, within PP_1124 at 1286.497 kb on + strand at 1286.497 kb on + strand at 1286.557 kb on - strand at 1286.557 kb on - strand at 1286.561 kb on + strand at 1286.561 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 1,283,813 + PP_1120 0.82 +0.1 1,283,813 + PP_1120 0.82 -0.9 1,283,818 - PP_1120 0.83 +0.2 1,283,977 + +0.3 1,283,977 + -1.0 1,283,977 + +0.3 1,283,977 + -0.2 1,283,978 - +0.1 1,283,978 - -0.5 1,283,978 - +1.5 1,283,982 + -0.1 1,283,982 + +1.0 1,283,982 + -0.0 1,283,983 - +1.4 1,283,983 - -0.4 1,284,013 + +0.5 1,284,013 + +0.4 1,284,013 + -0.3 1,284,070 + -1.6 1,284,070 + +0.5 1,284,071 - -2.1 1,284,071 - -2.8 1,284,071 - +0.3 1,284,071 - +0.1 1,284,073 + -0.4 1,284,073 + -1.0 1,284,073 + -0.4 1,284,073 + -2.4 1,284,073 + +0.6 1,284,074 - -3.7 1,284,074 - -1.2 1,284,094 + +0.3 1,284,173 - PP_1121 0.16 +0.7 1,284,173 - PP_1121 0.16 -1.1 1,284,173 - PP_1121 0.16 +0.2 1,284,173 - PP_1121 0.16 -0.1 1,284,173 - PP_1121 0.16 -1.0 1,284,255 + PP_1121 0.28 -0.1 1,284,255 + PP_1121 0.28 -0.5 1,284,489 - PP_1121 0.61 +1.2 1,284,534 - PP_1121 0.68 -0.3 1,284,534 - PP_1121 0.68 +0.7 1,284,573 + PP_1121 0.74 -0.9 1,284,574 - PP_1121 0.74 -0.5 1,284,574 - PP_1121 0.74 -0.8 1,284,574 - PP_1121 0.74 -0.4 1,284,574 - PP_1121 0.74 +0.7 1,284,732 - -0.4 1,284,753 + -0.2 1,285,068 - PP_1122 0.37 +0.7 1,285,388 + PP_1122 0.78 +1.0 1,285,649 + -0.7 1,285,649 + +0.0 1,285,649 + -0.7 1,285,649 + +0.8 1,285,649 + -0.2 1,285,649 + -2.4 1,285,649 + +0.8 1,285,649 + +0.3 1,285,649 + +0.1 1,285,649 + +0.0 1,285,649 + +0.7 1,285,650 - +0.7 1,285,650 - +0.2 1,285,650 - -0.6 1,285,650 - -0.1 1,285,650 - -0.5 1,285,650 - -0.8 1,285,650 - +1.4 1,285,650 - -0.3 1,285,650 - +0.5 1,285,650 - +1.3 1,285,662 + -0.1 1,286,266 + PP_1124 0.45 -0.7 1,286,267 - PP_1124 0.46 -0.6 1,286,267 - PP_1124 0.46 -0.6 1,286,322 + PP_1124 0.57 -1.7 1,286,322 + PP_1124 0.57 -0.1 1,286,469 + PP_1124 0.89 +0.5 1,286,497 + +1.1 1,286,497 + +0.1 1,286,557 - -0.4 1,286,557 - -0.1 1,286,561 + +0.4 1,286,561 + +1.9
Or see this region's nucleotide sequence