Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0988 and PP_0989 are separated by 11 nucleotides PP_0989 and PP_0990 are separated by 303 nucleotides PP_0990 and PP_0991 are separated by 73 nucleotides
PP_0988: PP_0988 - glycine dehydrogenase, at 1,126,695 to 1,129,550
_0988
PP_0989: PP_0989 - glycine cleavage system H protein 1, at 1,129,562 to 1,129,945
_0989
PP_0990: PP_0990 - DoxX family protein, at 1,130,249 to 1,130,635
_0990
PP_0991: PP_0991 - conserved protein of unknown function, at 1,130,709 to 1,131,482
_0991
Position (kb)
1129
1130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1128.703 kb on + strand, within PP_0988 at 1128.704 kb on - strand, within PP_0988 at 1129.217 kb on - strand, within PP_0988 at 1129.225 kb on + strand, within PP_0988 at 1129.225 kb on + strand, within PP_0988 at 1129.226 kb on - strand, within PP_0988 at 1129.234 kb on + strand, within PP_0988 at 1129.235 kb on - strand, within PP_0988 at 1129.291 kb on + strand at 1129.291 kb on + strand at 1129.292 kb on - strand at 1129.292 kb on - strand at 1129.292 kb on - strand at 1129.292 kb on - strand at 1129.292 kb on - strand at 1129.294 kb on + strand at 1129.309 kb on + strand at 1129.503 kb on + strand at 1129.504 kb on - strand at 1129.504 kb on - strand at 1129.504 kb on - strand at 1129.596 kb on + strand at 1129.749 kb on + strand, within PP_0989 at 1129.749 kb on + strand, within PP_0989 at 1129.749 kb on + strand, within PP_0989 at 1129.749 kb on + strand, within PP_0989 at 1129.864 kb on - strand, within PP_0989 at 1130.002 kb on - strand at 1130.002 kb on - strand at 1130.083 kb on - strand at 1130.086 kb on + strand at 1130.087 kb on - strand at 1130.207 kb on + strand at 1130.493 kb on - strand, within PP_0990 at 1130.493 kb on - strand, within PP_0990 at 1130.505 kb on + strand, within PP_0990 at 1130.506 kb on - strand, within PP_0990 at 1130.526 kb on + strand, within PP_0990 at 1130.526 kb on + strand at 1130.526 kb on + strand, within PP_0990 at 1130.526 kb on + strand, within PP_0990 at 1130.526 kb on + strand, within PP_0990 at 1130.527 kb on - strand, within PP_0990 at 1130.527 kb on - strand, within PP_0990 at 1130.527 kb on - strand, within PP_0990 at 1130.527 kb on - strand, within PP_0990 at 1130.539 kb on + strand, within PP_0990 at 1130.539 kb on + strand, within PP_0990 at 1130.539 kb on + strand at 1130.540 kb on - strand, within PP_0990 at 1130.540 kb on - strand, within PP_0990 at 1130.901 kb on + strand, within PP_0991 at 1130.901 kb on + strand, within PP_0991 at 1130.901 kb on + strand, within PP_0991 at 1130.902 kb on - strand, within PP_0991 at 1130.902 kb on - strand, within PP_0991
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 1,128,703 + PP_0988 0.70 -0.0 1,128,704 - PP_0988 0.70 -0.7 1,129,217 - PP_0988 0.88 +0.2 1,129,225 + PP_0988 0.89 -1.1 1,129,225 + PP_0988 0.89 -0.8 1,129,226 - PP_0988 0.89 +0.8 1,129,234 + PP_0988 0.89 +0.2 1,129,235 - PP_0988 0.89 +0.4 1,129,291 + +0.6 1,129,291 + +0.7 1,129,292 - +0.5 1,129,292 - +0.4 1,129,292 - +0.4 1,129,292 - -0.2 1,129,292 - -0.3 1,129,294 + -0.8 1,129,309 + +0.5 1,129,503 + -1.9 1,129,504 - -1.2 1,129,504 - -2.4 1,129,504 - +0.6 1,129,596 + +1.3 1,129,749 + PP_0989 0.49 +0.3 1,129,749 + PP_0989 0.49 +0.7 1,129,749 + PP_0989 0.49 +1.7 1,129,749 + PP_0989 0.49 -0.8 1,129,864 - PP_0989 0.79 +0.4 1,130,002 - +0.4 1,130,002 - -0.7 1,130,083 - +0.3 1,130,086 + -0.2 1,130,087 - +0.8 1,130,207 + -0.2 1,130,493 - PP_0990 0.63 +0.4 1,130,493 - PP_0990 0.63 -0.4 1,130,505 + PP_0990 0.66 -1.8 1,130,506 - PP_0990 0.66 -0.1 1,130,526 + PP_0990 0.72 -1.2 1,130,526 + -1.1 1,130,526 + PP_0990 0.72 -0.4 1,130,526 + PP_0990 0.72 -0.6 1,130,526 + PP_0990 0.72 +1.4 1,130,527 - PP_0990 0.72 +0.2 1,130,527 - PP_0990 0.72 +0.1 1,130,527 - PP_0990 0.72 -0.1 1,130,527 - PP_0990 0.72 -0.2 1,130,539 + PP_0990 0.75 +0.3 1,130,539 + PP_0990 0.75 +0.1 1,130,539 + +2.4 1,130,540 - PP_0990 0.75 -2.6 1,130,540 - PP_0990 0.75 -0.0 1,130,901 + PP_0991 0.25 -1.3 1,130,901 + PP_0991 0.25 -1.4 1,130,901 + PP_0991 0.25 +2.0 1,130,902 - PP_0991 0.25 +0.3 1,130,902 - PP_0991 0.25 -0.6
Or see this region's nucleotide sequence