Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0802 and PP_0803 are separated by 92 nucleotides PP_0803 and PP_0804 overlap by 4 nucleotides
PP_0802: PP_0802 - putative chemotaxis protein, at 920,851 to 921,750
_0802
PP_0803: PP_0803 - Protein secretion ABC efflux system, membrane fusion protein, at 921,843 to 923,027
_0803
PP_0804: PP_0804 - Protein secretion ABC efflux system, permease and ATP-binding protein, at 923,024 to 925,192
_0804
Position (kb) 921
922
923
924 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 920.847 kb on + strand at 920.847 kb on + strand at 920.847 kb on + strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.848 kb on - strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.849 kb on + strand at 920.850 kb on - strand at 920.925 kb on + strand at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.002 kb on + strand, within PP_0802 at 921.003 kb on - strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.237 kb on + strand, within PP_0802 at 921.319 kb on + strand, within PP_0802 at 921.460 kb on + strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.461 kb on - strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.601 kb on + strand, within PP_0802 at 921.602 kb on - strand, within PP_0802 at 921.602 kb on - strand, within PP_0802 at 922.021 kb on + strand, within PP_0803 at 922.021 kb on + strand, within PP_0803 at 922.068 kb on + strand, within PP_0803 at 922.093 kb on + strand, within PP_0803 at 922.094 kb on - strand, within PP_0803 at 922.094 kb on - strand, within PP_0803 at 922.096 kb on - strand, within PP_0803 at 922.096 kb on - strand, within PP_0803 at 922.103 kb on - strand, within PP_0803 at 922.103 kb on - strand, within PP_0803 at 922.108 kb on + strand, within PP_0803 at 922.108 kb on + strand, within PP_0803 at 922.109 kb on - strand, within PP_0803 at 922.400 kb on - strand, within PP_0803 at 922.588 kb on + strand, within PP_0803 at 922.588 kb on + strand, within PP_0803 at 922.589 kb on - strand, within PP_0803 at 922.589 kb on - strand, within PP_0803 at 922.706 kb on - strand, within PP_0803 at 922.999 kb on + strand at 923.000 kb on - strand at 923.158 kb on + strand at 923.682 kb on + strand, within PP_0804 at 923.683 kb on - strand, within PP_0804
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 920,847 + -0.3 920,847 + +1.0 920,847 + -0.1 920,848 - -0.0 920,848 - +0.6 920,848 - +0.1 920,849 + +0.7 920,849 + -0.5 920,849 + -0.0 920,849 + +0.2 920,849 + +0.3 920,849 + +0.1 920,850 - -0.5 920,925 + -1.6 921,002 + PP_0802 0.17 -1.6 921,002 + PP_0802 0.17 -2.8 921,002 + PP_0802 0.17 -0.2 921,002 + PP_0802 0.17 -1.4 921,003 - PP_0802 0.17 +0.1 921,237 + PP_0802 0.43 +0.8 921,237 + PP_0802 0.43 -1.3 921,319 + PP_0802 0.52 -0.2 921,460 + PP_0802 0.68 -0.1 921,461 - PP_0802 0.68 +0.8 921,461 - PP_0802 0.68 +0.5 921,601 + PP_0802 0.83 -0.7 921,601 + PP_0802 0.83 -0.3 921,602 - PP_0802 0.83 -0.6 921,602 - PP_0802 0.83 +0.3 922,021 + PP_0803 0.15 -1.3 922,021 + PP_0803 0.15 +1.5 922,068 + PP_0803 0.19 +0.4 922,093 + PP_0803 0.21 +0.1 922,094 - PP_0803 0.21 +0.1 922,094 - PP_0803 0.21 +2.1 922,096 - PP_0803 0.21 -0.1 922,096 - PP_0803 0.21 -0.4 922,103 - PP_0803 0.22 +1.8 922,103 - PP_0803 0.22 +0.2 922,108 + PP_0803 0.22 +0.9 922,108 + PP_0803 0.22 -0.3 922,109 - PP_0803 0.22 +0.1 922,400 - PP_0803 0.47 +0.6 922,588 + PP_0803 0.63 +0.5 922,588 + PP_0803 0.63 -0.1 922,589 - PP_0803 0.63 +0.7 922,589 - PP_0803 0.63 +1.1 922,706 - PP_0803 0.73 +1.4 922,999 + +0.0 923,000 - +1.9 923,158 + -0.8 923,682 + PP_0804 0.30 +2.3 923,683 - PP_0804 0.30 +0.5
Or see this region's nucleotide sequence