Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0397 and PP_0398 are separated by 282 nucleotides PP_0398 and PP_0399 are separated by 14 nucleotides PP_0399 and PP_0400 overlap by 1 nucleotides
PP_0397: PP_0397 - protein kinase, at 482,516 to 484,438
_0397
PP_0398: PP_0398 - Thiosulfate sulfurtransferase GlpE, at 484,721 to 485,053
_0398
PP_0399: PP_0399 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 485,068 to 485,934
_0399
PP_0400: PP_0400 - CO2+/MG2+ efflux protein, at 485,934 to 486,314
_0400
Position (kb) 484
485
486 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 483.741 kb on + strand, within PP_0397 at 483.839 kb on - strand, within PP_0397 at 483.939 kb on + strand, within PP_0397 at 484.041 kb on + strand, within PP_0397 at 484.041 kb on + strand, within PP_0397 at 484.120 kb on - strand, within PP_0397 at 484.120 kb on - strand, within PP_0397 at 484.167 kb on + strand, within PP_0397 at 484.168 kb on - strand, within PP_0397 at 484.235 kb on - strand, within PP_0397 at 484.273 kb on + strand at 484.274 kb on - strand at 484.274 kb on - strand at 484.274 kb on - strand at 484.400 kb on - strand at 484.400 kb on - strand at 484.432 kb on + strand at 484.456 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.520 kb on + strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.521 kb on - strand at 484.522 kb on + strand at 484.542 kb on - strand at 484.542 kb on - strand at 484.604 kb on - strand at 484.604 kb on - strand at 484.609 kb on + strand at 484.609 kb on + strand at 484.609 kb on + strand at 484.609 kb on + strand at 484.610 kb on - strand at 484.618 kb on - strand at 484.629 kb on - strand at 484.629 kb on - strand at 484.629 kb on - strand at 484.653 kb on - strand at 484.794 kb on + strand, within PP_0398 at 484.817 kb on + strand, within PP_0398 at 484.824 kb on + strand, within PP_0398 at 484.825 kb on - strand, within PP_0398 at 484.858 kb on - strand, within PP_0398 at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.060 kb on + strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.061 kb on - strand at 485.088 kb on + strand at 485.088 kb on + strand at 485.089 kb on - strand at 485.089 kb on - strand at 485.089 kb on - strand at 486.017 kb on - strand, within PP_0400
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Polymyxin B sulfate 0.001 mg/ml remove 483,741 + PP_0397 0.64 -0.1 483,839 - PP_0397 0.69 -0.4 483,939 + PP_0397 0.74 -0.3 484,041 + PP_0397 0.79 +0.6 484,041 + PP_0397 0.79 -0.2 484,120 - PP_0397 0.83 -0.0 484,120 - PP_0397 0.83 -0.6 484,167 + PP_0397 0.86 -0.7 484,168 - PP_0397 0.86 +0.6 484,235 - PP_0397 0.89 +0.3 484,273 + -0.5 484,274 - -0.1 484,274 - +0.2 484,274 - +0.3 484,400 - -0.6 484,400 - +0.2 484,432 + -0.8 484,456 + -0.5 484,520 + -0.0 484,520 + -0.5 484,520 + -0.5 484,520 + -0.6 484,520 + +0.2 484,520 + +0.7 484,520 + +0.7 484,520 + -0.2 484,521 - -0.7 484,521 - -2.1 484,521 - -0.0 484,521 - +0.5 484,521 - -0.2 484,521 - +0.4 484,521 - -0.8 484,522 + -0.3 484,542 - +0.1 484,542 - -0.3 484,604 - -0.7 484,604 - -0.1 484,609 + -0.3 484,609 + -0.9 484,609 + -0.4 484,609 + +0.7 484,610 - +0.2 484,618 - -0.5 484,629 - -2.0 484,629 - -0.3 484,629 - -0.9 484,653 - -0.5 484,794 + PP_0398 0.22 -0.2 484,817 + PP_0398 0.29 +0.1 484,824 + PP_0398 0.31 +0.1 484,825 - PP_0398 0.31 +0.3 484,858 - PP_0398 0.41 +1.8 485,060 + +0.2 485,060 + +1.2 485,060 + +4.3 485,060 + +0.2 485,060 + -0.0 485,061 - -0.2 485,061 - -0.8 485,061 - +1.3 485,061 - +1.1 485,061 - +1.2 485,088 + +0.1 485,088 + +0.3 485,089 - +0.8 485,089 - -1.1 485,089 - -0.2 486,017 - PP_0400 0.22 +0.0
Or see this region's nucleotide sequence