Strain Fitness in Pseudomonas putida KT2440 around PP_4850

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
5,516,676	+		PP_4849	0.38	+0.8
5,516,676	+		PP_4849	0.38	+1.4
5,516,677	-		PP_4849	0.38	-1.2
5,516,677	-		PP_4849	0.38	-0.5
5,516,703	+		PP_4849	0.40	+0.4
5,516,734	+		PP_4849	0.43	-0.8
5,516,910	+		PP_4849	0.57	-0.4
5,516,911	-		PP_4849	0.57	-0.9
5,516,952	+		PP_4849	0.60	-0.0
5,516,952	+		PP_4849	0.60	-0.2
5,516,953	-		PP_4849	0.60	-0.8
5,516,953	-		PP_4849	0.60	-1.1
5,516,995	-		PP_4849	0.63	+0.4
5,516,995	-		PP_4849	0.63	-0.3
5,517,034	-		PP_4849	0.66	+0.5
5,517,305	+		PP_4849	0.88	+0.7
5,517,398	+				+0.8
5,517,398	+				+0.2
5,517,399	-				-0.2
5,517,506	+				-0.1
5,517,506	+				-0.6
5,517,506	+				-1.3
5,517,506	+				-0.1
5,517,507	-				-0.9
5,517,507	-				+0.7
5,517,507	-				-0.4
5,517,507	-				-1.6
5,517,550	+				+0.4
5,517,551	-				+1.7
5,517,556	+				-1.8
5,517,556	+				-1.4
5,517,556	+				+1.0
5,517,556	+				-0.6
5,517,556	+				-0.8
5,517,556	+				-1.1
5,517,557	-				-0.4
5,517,557	-				-0.3
5,517,557	-				-0.3
5,517,557	-				+0.9
5,517,557	-				-0.2
5,517,557	-				-1.8
5,517,557	-				+0.1
5,517,557	-				-0.3
5,517,557	-				-0.1
5,517,557	-				+0.0
5,517,559	+				+0.8
5,517,559	+				-2.3
5,517,559	+				-1.3
5,517,559	+				-1.3
5,517,559	+				+0.7
5,517,559	+				+0.6
5,517,559	+				-0.3
5,517,559	+				-1.2
5,517,559	+				+0.0
5,517,560	-				-0.1
5,517,560	-				-0.7
5,517,560	-				+0.1
5,517,560	-				+0.9
5,517,560	-				-0.3
5,517,560	-				-1.5
5,517,560	-				+0.2
5,517,560	-				-0.0
5,517,606	+				-0.5
5,517,607	-				-0.1
5,517,679	+		PP_4850	0.16	-0.2
5,517,679	+		PP_4850	0.16	-0.8
5,517,849	+		PP_4850	0.31	-0.8
5,517,850	-		PP_4850	0.32	-0.7
5,517,850	-		PP_4850	0.32	-0.8
5,517,850	-		PP_4850	0.32	-0.7
5,518,171	+		PP_4850	0.62	-0.3
5,518,171	+		PP_4850	0.62	-1.0
5,518,520	+				-0.1
5,518,520	+				-3.9
5,518,521	-				-0.7
5,518,580	+				+1.3
5,518,928	+				+1.4
5,518,928	+				+0.0
5,518,983	-				+0.0
5,519,182	-		PP_4852	0.31	-0.4
5,519,322	+		PP_4852	0.48	-0.0
5,519,328	+				-0.8
5,519,467	+		PP_4852	0.67	-0.3
5,519,467	+		PP_4852	0.67	+0.7
5,519,468	-		PP_4852	0.67	+1.5
5,519,471	+		PP_4852	0.67	-0.1
5,519,471	+		PP_4852	0.67	+0.8
5,519,471	+		PP_4852	0.67	+0.0
5,519,471	+		PP_4852	0.67	-1.0
5,519,472	-		PP_4852	0.67	+0.6
5,519,472	-		PP_4852	0.67	-1.2
5,519,472	-		PP_4852	0.67	+0.9
5,519,472	-		PP_4852	0.67	+0.4
5,519,472	-		PP_4852	0.67	+0.2
5,519,472	-		PP_4852	0.67	-0.7
5,519,472	-		PP_4852	0.67	+1.0
5,519,472	-		PP_4852	0.67	-0.6
5,519,480	-		PP_4852	0.68	+0.5
5,519,500	+		PP_4852	0.71	-1.8
5,519,500	+		PP_4852	0.71	+0.2
5,519,501	-		PP_4852	0.71	-0.3
5,519,501	-		PP_4852	0.71	+0.1
5,519,501	-		PP_4852	0.71	-0.5
5,519,501	-		PP_4852	0.71	-0.1
5,519,501	-		PP_4852	0.71	-0.7
5,519,501	-		PP_4852	0.71	-0.0