Strain Fitness in Pseudomonas putida KT2440 around PP_4592

Experiment: R2A with Polymyxin B sulfate 0.001 mg/ml

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	R2A with Polymyxin B sulfate 0.001 mg/ml
5,209,420	+		PP_4591	0.37	-1.7
5,209,420	+		PP_4591	0.37	+0.1
5,209,427	-		PP_4591	0.38	-0.2
5,209,481	+		PP_4591	0.42	-0.6
5,209,481	+		PP_4591	0.42	-0.3
5,209,482	-		PP_4591	0.42	-0.6
5,209,482	-		PP_4591	0.42	-0.1
5,209,482	-		PP_4591	0.42	-0.0
5,209,606	-		PP_4591	0.53	-1.5
5,209,606	-		PP_4591	0.53	-1.9
5,209,606	-		PP_4591	0.53	+0.1
5,209,776	+		PP_4591	0.68	-3.4
5,209,777	-		PP_4591	0.68	-1.5
5,210,164	+				+0.9
5,210,164	+				-3.0
5,210,164	+				-0.4
5,210,164	+				-0.2
5,210,164	+				+2.1
5,210,164	+				+0.0
5,210,165	-				+0.7
5,210,165	-				-0.4
5,210,165	-				+0.2
5,210,167	-				-0.0
5,210,167	-				-1.1
5,210,168	+				-2.1
5,210,169	-				-1.0
5,210,169	-				-0.0
5,210,262	+				-0.5
5,210,262	+				-0.7
5,210,262	+				-1.3
5,210,263	-				-0.5
5,210,263	-				+0.3
5,210,263	-				-0.0
5,210,263	-				-0.2
5,210,370	+				-1.1
5,210,380	+				-0.6
5,210,381	-				-0.7
5,210,381	-				+0.1
5,210,381	-				-0.5
5,210,381	-				-0.6
5,210,384	+				-0.2
5,210,384	+				-0.5
5,210,385	-				-0.4
5,210,385	-				-0.7
5,210,385	-				-1.7
5,210,385	-				-2.7
5,210,385	-				+1.1
5,210,487	+		PP_4592	0.11	-4.1
5,210,487	+		PP_4592	0.11	-1.0
5,210,487	+		PP_4592	0.11	-0.5
5,210,488	-		PP_4592	0.11	-1.3
5,210,488	-		PP_4592	0.11	-0.6
5,210,516	+		PP_4592	0.12	+0.4
5,210,517	-		PP_4592	0.13	-1.1
5,210,517	-		PP_4592	0.13	-1.0
5,210,517	-		PP_4592	0.13	-1.0
5,210,548	-		PP_4592	0.14	-1.3
5,210,574	+		PP_4592	0.16	-0.1
5,210,574	+		PP_4592	0.16	+0.9
5,210,574	+		PP_4592	0.16	+1.7
5,210,574	+		PP_4592	0.16	-0.8
5,210,607	+		PP_4592	0.18	-0.3
5,210,607	+		PP_4592	0.18	-0.4
5,210,608	-		PP_4592	0.18	-1.3
5,210,680	+		PP_4592	0.22	-1.2
5,210,681	-		PP_4592	0.23	+0.6
5,210,682	+		PP_4592	0.23	-0.4
5,210,683	-		PP_4592	0.23	-0.6
5,210,683	-		PP_4592	0.23	-1.3
5,211,069	+		PP_4592	0.46	-0.1
5,211,069	+		PP_4592	0.46	-1.9
5,211,069	+		PP_4592	0.46	-3.3
5,211,069	+		PP_4592	0.46	-0.6
5,211,069	+		PP_4592	0.46	+0.1
5,211,310	-		PP_4592	0.61	-2.3
5,211,442	-		PP_4592	0.69	+0.3
5,211,442	-		PP_4592	0.69	-1.8
5,211,442	-		PP_4592	0.69	-1.8
5,211,442	-		PP_4592	0.69	-1.6
5,211,442	-		PP_4592	0.69	-4.6
5,211,442	-		PP_4592	0.69	-0.4
5,211,583	+		PP_4592	0.78	-0.2
5,211,583	+				-0.1
5,211,584	-		PP_4592	0.78	+1.2
5,211,585	+		PP_4592	0.78	-0.6
5,211,585	+		PP_4592	0.78	+0.0
5,211,585	+		PP_4592	0.78	+0.1
5,211,586	-		PP_4592	0.78	-0.9
5,211,586	-		PP_4592	0.78	-1.0
5,211,586	-		PP_4592	0.78	-0.7
5,211,646	+		PP_4592	0.81	-0.5
5,211,667	+		PP_4592	0.83	-0.9
5,211,667	+		PP_4592	0.83	-0.9
5,211,668	-		PP_4592	0.83	+0.1
5,211,668	-		PP_4592	0.83	+0.7
5,211,671	+		PP_4592	0.83	+0.3
5,211,678	+		PP_4592	0.83	-0.5
5,211,693	+		PP_4592	0.84	+0.5
5,211,693	+		PP_4592	0.84	-1.9
5,211,694	-		PP_4592	0.84	-0.6
5,211,727	+		PP_4592	0.86	-0.3
5,211,727	+		PP_4592	0.86	-1.9
5,211,727	+		PP_4592	0.86	-0.6
5,211,728	-		PP_4592	0.86	-1.8
5,211,731	+		PP_4592	0.87	-0.1
5,211,732	-		PP_4592	0.87	-1.1
5,211,732	-		PP_4592	0.87	-0.4
5,211,732	-		PP_4592	0.87	-0.1
5,211,743	+		PP_4592	0.87	-1.2
5,211,743	+		PP_4592	0.87	-0.3
5,211,743	+		PP_4592	0.87	-1.5
5,211,743	+		PP_4592	0.87	-0.7
5,211,743	+		PP_4592	0.87	-2.3
5,211,744	-		PP_4592	0.87	-0.2
5,211,744	-		PP_4592	0.87	-0.5
5,211,747	+		PP_4592	0.88	-0.8
5,211,762	-		PP_4592	0.89	-1.7
5,211,762	-		PP_4592	0.89	-0.5
5,211,874	+				-3.7
5,211,874	+				-2.8
5,211,874	+				+0.7
5,211,874	+				-1.2
5,211,874	+				-1.6
5,211,874	+				-0.8
5,211,875	-				+0.0
5,211,875	-				-0.0
5,211,875	-				-0.7
5,211,875	-				-1.0
5,211,875	-				-0.5
5,211,875	-				-1.1
5,211,895	-				-0.8
5,211,895	-				-3.1
5,211,961	-				-0.4
5,212,017	+				-1.0
5,212,081	-				-0.5
5,212,151	+				-0.6
5,212,151	+				-1.0
5,212,151	+				-1.8
5,212,151	+				-0.5
5,212,172	+				-0.9
5,212,302	+		PP_4593	0.43	+1.4
5,212,303	-		PP_4593	0.43	-0.8
5,212,445	+				-0.8
5,212,446	-				+0.7
5,212,484	+				+0.6
5,212,485	-				+0.1
5,212,485	-				-0.4
5,212,485	-				+0.3
5,212,494	+				-0.2
5,212,494	+				-0.8
5,212,495	-				-0.0
5,212,779	+		PP_4594	0.21	+0.8
5,212,864	+		PP_4594	0.28	-0.1
5,212,864	+		PP_4594	0.28	-0.2
5,212,865	-		PP_4594	0.28	-0.2
5,212,865	-		PP_4594	0.28	+0.7
5,212,865	-		PP_4594	0.28	+0.1
5,212,865	-		PP_4594	0.28	-0.9